Q6M0H5 (KHSE_METMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	MMP0295

Organism

Methanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]

Taxonomic identifier

267377 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Ref.2
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. Ref.2
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Homoserine kinase HAMAP MF_00384		PRO_0000156586
Regions
	Nucleotide binding	89 – 99	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6M0H5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 232945428ED59CBC	FASTA	301	32,453
	10 20 30 40 50 60 MKKVKVCSPG TSANLGPGYD IFGLALSNPY DIVEVEKTEN GITISVEGEK AEEIPTNVDE 70 80 90 100 110 120 NTAGVVAKKM IEDFKIESGI HIHIIKGIKP GSGLGSSSAS CAGIAFALNE LFELKLSKLE 130 140 150 160 170 180 LVKYSSLGEA VAAGAPHADN VAPAIFGGFT LTTSYEPLEV LHIPVDLEVL VALPNIQVST 190 200 210 220 230 240 KTAREILPKE IPIKDMVNNV GKAAGMVYAL YNNDLDLFGR YMSKDCVVEP CRANLIDGYT 250 260 270 280 290 300 EVKEKVKDMV YGITISGSGP AIITIPKKEH VIDIENIFKE VWNCPVYYTK VGPGCYVEEI E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.
[2]	"Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase." Graham D.E., Taylor S.M., Wolf R.Z., Namboori S.C. Biochem. J. 424:467-478(2009) [PubMed: 19761441] [Abstract] Cited for: FUNCTION AS A HOMOSERINE KINASE, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950229 Genomic DNA. Translation: CAF29851.1.
RefSeq	NP_987415.1. NC_005791.1.
3D structure databases
ProteinModelPortal	Q6M0H5.
SMR	Q6M0H5. Positions 1-297.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2761624.
GenomeReviews	Gene locus MMP0295 in contig BX950229_GR.
KEGG	mmp:MMP0295.
NMPDR	fig|267377.1.peg.295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646290.
OMA	GSAHADN.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0295-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_METMP

Accession

Primary (citable) accession number: Q6M0H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program