Q6M0G7 (AGOG_METMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-glycosylase/DNA lyase

Alternative name(s):
8-oxoguanine DNA glycosylase
EC=3.2.2.-
AGOG
DNA-(apurinic or apyrimidinic site) lyase
Short name=AP lyase
EC=4.2.99.18

Gene names

Ordered Locus Names:

MMP0304

Organism

Methanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]

Taxonomic identifier

267377 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus › Methanococcus maripaludis

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity. HAMAP-Rule MF_01168
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_01168
Domain	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). HAMAP-Rule MF_01168
Sequence similarities	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair
Molecular function	Hydrolase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Molecular_function	DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: HAMAP oxidized base lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	N-glycosylase/DNA lyase HAMAP-Rule MF_01168		PRO_0000185109
Regions
	Region	129 – 193	65	Helix-hairpin-helix HAMAP-Rule MF_01168
Sites
	Active site	153	1	Schiff-base intermediate with DNA By similarity
	Active site	185	1	Potential
	Binding site	32	1	8-oxoguanine By similarity
	Binding site	60	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	71	1	8-oxoguanine By similarity
	Binding site	157	1	8-oxoguanine By similarity
	Binding site	183	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	219	1	8-oxoguanine By similarity
	Binding site	223	1	8-oxoguanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6M0G7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 400E65728A8E63E9	FASTA	252	30,106
	10 20 30 40 50 60 MRNLEKINEL LEIFGHFDVN FAKTMEEKID TQYFVLENLK NSMNNDEMFI KLVILNSIVS 70 80 90 100 110 120 YQLCTTGELW WEEFSKYWSK HDANNENLGE SYVNFLENSK GNKRLLNVKI KRIERITPFL 130 140 150 160 170 180 ENLNLLDFKT YYSDMEKLLE NLSKYLNSKK NSKTVVFAVK MFGYASRIVF NEFFPYPMNI 190 200 210 220 230 240 EIPKDSRIEK YTLKFTDENP IKFWNEVSKT AKIPPLHIDS IIWPVLGRNF DFKSCENKLD 250 ENFRYLLKLT EL

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950229 Genomic DNA. Translation: CAF29860.1.
RefSeq	NP_987424.1. NC_005791.1.
3D structure databases
ProteinModelPortal	Q6M0G7.
ModBase	Search...
Protein-protein interaction databases
STRING	267377.MMP0304.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAF29860; CAF29860; MMP0304.
GeneID	2761855.
KEGG	mmp:MMP0304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4047.
HOGENOM	HOG000254352.
KO	K01741.
OMA	VKMFGYA.
ProtClustDB	PRK13280.
Enzyme and pathway databases
BioCyc	MMAR267377:GJ77-326-MONOMER.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit.
HAMAP	MF_01168. AGOG.
InterPro	IPR011257. DNA_glycosylase. IPR023170. HTH_base_excis_C. IPR015254. N-Glyclase/DNA_lyase-like_arc. IPR016544. N-Glyclase/DNA_lyase_arc. [Graphical view]
Pfam	PF09171. DUF1886. 1 hit. [Graphical view]
PIRSF	PIRSF008955. AGOG. 1 hit.
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGOG_METMP

Accession

Primary (citable) accession number: Q6M0G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program