Reviewed, UniProtKB/Swiss-Prot Q6M0G7 (AGOG_METMP)

Names and origin

Protein names

Recommended name:
    N-glycosylase/DNA lyase
Alternative name(s):
    AGOG
    8-oxoguanine DNA glycosylase
    EC=3.2.2.-
    DNA-(apurinic or apyrimidinic site) lyase
      Short name=AP lyase
    EC=4.2.99.18

Gene names

Ordered Locus Names:

MMP0304

Organism

Methanococcus maripaludis [Complete proteome] [HAMAP]

Taxonomic identifier

39152 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity.
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01168
Domain	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). HAMAP MF_01168
Sequence similarities	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair
Molecular function	Hydrolase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: HAMAP oxidized base lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	N-glycosylase/DNA lyase HAMAP MF_01168		PRO_0000185109
Regions
	Region	129 – 193	65	Helix-hairpin-helix HAMAP MF_01168
Sites
	Active site	153	1	Schiff-base intermediate with DNA By similarity
	Active site	185	1	Potential
	Binding site	32	1	8-oxoguanine By similarity
	Binding site	60	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	71	1	8-oxoguanine By similarity
	Binding site	157	1	8-oxoguanine By similarity
	Binding site	183	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	219	1	8-oxoguanine By similarity
	Binding site	223	1	8-oxoguanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6M0G7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 400E65728A8E63E9	FASTA	252	30,106
	10 20 30 40 50 60 MRNLEKINEL LEIFGHFDVN FAKTMEEKID TQYFVLENLK NSMNNDEMFI KLVILNSIVS 70 80 90 100 110 120 YQLCTTGELW WEEFSKYWSK HDANNENLGE SYVNFLENSK GNKRLLNVKI KRIERITPFL 130 140 150 160 170 180 ENLNLLDFKT YYSDMEKLLE NLSKYLNSKK NSKTVVFAVK MFGYASRIVF NEFFPYPMNI 190 200 210 220 230 240 EIPKDSRIEK YTLKFTDENP IKFWNEVSKT AKIPPLHIDS IIWPVLGRNF DFKSCENKLD 250 ENFRYLLKLT EL

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950229 Genomic DNA. Translation: CAF29860.1.
RefSeq	NP_987424.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2761855.
GenomeReviews	Gene locus MMP0304 in contig BX950229_GR.
KEGG	mmp:MMP0304.
NMPDR	fig|267377.1.peg.304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6M0G7.
OMA	PLHIDSI.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0304-MON.
BRENDA	4.2.99.18. 20956.
Family and domain databases
HAMAP	MF_01168. [Tree]
InterPro	IPR015254. N-Glyclase/DNA_lyase-like_arc. IPR016544. N-Glyclase/DNA_lyase_arc. [Graphical view]
Pfam	PF09171. DUF1886. 1 hit. [Graphical view]
PIRSF	PIRSF008955. AGOG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGOG_METMP

Accession

Primary (citable) accession number: Q6M0G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)