Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-glycosylase/DNA lyase

Gene

MMP0304

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei328-oxoguanineUniRule annotation1
Binding sitei608-oxoguanine; via carbonyl oxygenUniRule annotation1
Binding sitei718-oxoguanineUniRule annotation1
Active sitei153Schiff-base intermediate with DNAUniRule annotation1
Binding sitei1578-oxoguanineUniRule annotation1
Binding sitei1838-oxoguanine; via carbonyl oxygenUniRule annotation1
Active sitei185UniRule annotation1
Binding sitei2198-oxoguanineUniRule annotation1
Binding sitei2238-oxoguanineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
Biological processDNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciMMAR267377:G1G3S-326-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:MMP0304
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000590 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001851091 – 252N-glycosylase/DNA lyaseAdd BLAST252

Interactioni

Protein-protein interaction databases

STRINGi267377.MMP0304

Structurei

3D structure databases

ProteinModelPortaliQ6M0G7
SMRiQ6M0G7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 193Helix-hairpin-helixAdd BLAST65

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04144 Archaea
COG4047 LUCA
HOGENOMiHOG000254352
KOiK01741
OMAiVKMFGYA
OrthoDBiPOG093Z09FD

Family and domain databases

Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_01168 AGOG, 1 hit
InterProiView protein in InterPro
IPR016544 AGOG
IPR015254 AGOG-like
IPR011257 DNA_glycosylase
IPR023170 HTH_base_excis_C
PfamiView protein in Pfam
PF09171 AGOG, 1 hit
PIRSFiPIRSF008955 AGOG, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit

Sequencei

Sequence statusi: Complete.

Q6M0G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNLEKINEL LEIFGHFDVN FAKTMEEKID TQYFVLENLK NSMNNDEMFI
60 70 80 90 100
KLVILNSIVS YQLCTTGELW WEEFSKYWSK HDANNENLGE SYVNFLENSK
110 120 130 140 150
GNKRLLNVKI KRIERITPFL ENLNLLDFKT YYSDMEKLLE NLSKYLNSKK
160 170 180 190 200
NSKTVVFAVK MFGYASRIVF NEFFPYPMNI EIPKDSRIEK YTLKFTDENP
210 220 230 240 250
IKFWNEVSKT AKIPPLHIDS IIWPVLGRNF DFKSCENKLD ENFRYLLKLT

EL
Length:252
Mass (Da):30,106
Last modified:July 5, 2004 - v1
Checksum:i400E65728A8E63E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA Translation: CAF29860.1
RefSeqiWP_011170248.1, NC_005791.1

Genome annotation databases

EnsemblBacteriaiCAF29860; CAF29860; MMP0304
GeneIDi2761855
KEGGimmp:MMP0304
PATRICifig|267377.15.peg.307

Similar proteinsi

Entry informationi

Entry nameiAGOG_METMP
AccessioniPrimary (citable) accession number: Q6M0G7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health