Q6M0F3 (ILVD_METMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	MMP0318

Organism

Methanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]

Taxonomic identifier

267377 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103544
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6M0F3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: CF8730D4B4C53259	FASTA	550	58,718
	10 20 30 40 50 60 MISDNVKKGV IRTPNRALLK ACGYTDEDME KPFIGIVNSF TEVVPGHIHL RTLSEAAKHG 70 80 90 100 110 120 VYANGGTPFE FNTIGICDGI AMGHEGMKYS LPSREIIADA VESMARAHGF DGLVLIPTCD 130 140 150 160 170 180 KIVPGMIMGA LRLNIPFIVV TGGPMLPGEF QGKKYELISL FEGVGEYQVG KITEEELKCI 190 200 210 220 230 240 EDCACSGAGS CAGLYTANSM ACLTEALGLS LPMCATTHAV DAQKVRLAKK SGSKIVDMVK 250 260 270 280 290 300 EDLKPTDILT KEAFENAILV DLALGGSTNT TLHIPAIANE IENKFITLDD FDRLSDEVPH 310 320 330 340 350 360 IASIKPGGEH YMIDLHNAGG IPAVLNVLKE KIRDTKTVDG RSILEIAESV KYINYDVIRK 370 380 390 400 410 420 VEAPVHETAG LRVLKGNLAP NGCVVKIGAV HPKMYKHDGP AKVYNSEDEA ISAILGGKIV 430 440 450 460 470 480 EGDVIVIRYE GPSGGPGMRE MLSPTSAICG MGLDDSVALI TDGRFSGGSR GPCIGHVSPE 490 500 510 520 530 540 AAAGGVIAAI ENGDIIKIDM IEKEINVDLD ESVIKERLSK LGEFEPKIKK GYLSRYSKLV 550 SSADEGAVLK

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950229 Genomic DNA. Translation: CAF29874.1.
RefSeq	NP_987438.1. NC_005791.1.
3D structure databases
ProteinModelPortal	Q6M0F3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2762188.
GenomeReviews	Gene locus MMP0318 in contig BX950229_GR.
KEGG	mmp:MMP0318.
NMPDR	fig|267377.1.peg.318.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0318-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_METMP

Accession

Primary (citable) accession number: Q6M0F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program