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Q6M056 (KAE1B_METMP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.6.99.4
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name=t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:MMP0415
OrganismMethanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]
Taxonomic identifier267377 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303651

Regions

Domain347 – 548202Protein kinase
Nucleotide binding353 – 3619ATP By similarity
Region1 – 330330Kae1 HAMAP-Rule MF_01447
Region134 – 1385Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4661Proton acceptor; for kinase activity By similarity
Metal binding1131Iron By similarity
Metal binding1171Iron By similarity
Metal binding1341Iron By similarity
Metal binding2911Iron By similarity
Binding site1661Threonylcarbamoyl-AMP By similarity
Binding site1791Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1831Threonylcarbamoyl-AMP By similarity
Binding site2631Threonylcarbamoyl-AMP By similarity
Binding site3741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6M056 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: F3EB6F358157768B

FASTA54861,248
        10         20         30         40         50         60 
MDNSKDDLIC IGFEGTAEKS GVGIITSKGE VLFNKTIIYT PPVQGIHPRE AADHHAETFV 

        70         80         90        100        110        120 
KLLKEALNEV PLEKIDLVSF SLGPGLGPSL RVTATTARAL SLSINKPIIG VNHCIGHVEI 

       130        140        150        160        170        180 
GKLTTDAVDP LTLYVSGGNT QVLAYTGKKY RVIGETLDIA IGNCLDQFAR HCNLPHPGGV 

       190        200        210        220        230        240 
YVEKFAKDGN KFIKLPYTVK GMDLSLSGLL TSAMKKYDSK ERIEDVCYSL QETSFSMLTE 

       250        260        270        280        290        300 
ITERALAHTN KAEVMLVGGV AANNRLKEML KVMCEEQNVD FYVPEKQFCG DNGAMIAWLG 

       310        320        330        340        350        360 
ILQYLNGKRM DLKDTKPISN YRSDMVEVNW IHDESKNLND GNIKSRKIPK HLIGKGAEAD 

       370        380        390        400        410        420 
ISKGRYLEFE SITKERVKKG YRILKLDELI RTRRTVKEAR FLAAVKECGI HAPSIYDIDK 

       430        440        450        460        470        480 
ENKKIIMGYI HGKIAKDEIE EGNIEFCKSL GETIGKMHGS GIVHNDLTTS NFIISKNPYI 

       490        500        510        520        530        540 
IDFGLGKYSD LIEDKAIDLI VLKKSIMSIH YDKFDLVWNK IIDGYKTYEL AESVLECIKE 


VEKRARYL 

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References

[1]"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis."
Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. expand/collapse author list , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A.
J. Bacteriol. 186:6956-6969(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S2 / LL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950229 Genomic DNA. Translation: CAF29971.1.
RefSeqNP_987535.1. NC_005791.1.

3D structure databases

ProteinModelPortalQ6M056.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267377.MMP0415.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAF29971; CAF29971; MMP0415.
GeneID2762045.
KEGGmmp:MMP0415.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.

Enzyme and pathway databases

BioCycMMAR267377:GJ77-442-MONOMER.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_METMP
AccessionPrimary (citable) accession number: Q6M056
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families