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Q6M056

- KAE1B_METMP

UniProt

Q6M056 - KAE1B_METMP

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

MMP0415

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131IronUniRule annotation
    Metal bindingi117 – 1171IronUniRule annotation
    Metal bindingi134 – 1341IronUniRule annotation
    Binding sitei166 – 1661Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei179 – 1791Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei183 – 1831Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei263 – 2631Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi291 – 2911IronUniRule annotation
    Binding sitei374 – 3741ATPUniRule annotation
    Active sitei466 – 4661Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi353 – 3619ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. protein tyrosine kinase activity Source: InterPro
    7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-442-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:MMP0415
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 548548Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303651Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi267377.MMP0415.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6M056.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini347 – 548202Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 330330Kae1Add
    BLAST
    Regioni134 – 1385Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109569.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6M056-1 [UniParc]FASTAAdd to Basket

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    MDNSKDDLIC IGFEGTAEKS GVGIITSKGE VLFNKTIIYT PPVQGIHPRE    50
    AADHHAETFV KLLKEALNEV PLEKIDLVSF SLGPGLGPSL RVTATTARAL 100
    SLSINKPIIG VNHCIGHVEI GKLTTDAVDP LTLYVSGGNT QVLAYTGKKY 150
    RVIGETLDIA IGNCLDQFAR HCNLPHPGGV YVEKFAKDGN KFIKLPYTVK 200
    GMDLSLSGLL TSAMKKYDSK ERIEDVCYSL QETSFSMLTE ITERALAHTN 250
    KAEVMLVGGV AANNRLKEML KVMCEEQNVD FYVPEKQFCG DNGAMIAWLG 300
    ILQYLNGKRM DLKDTKPISN YRSDMVEVNW IHDESKNLND GNIKSRKIPK 350
    HLIGKGAEAD ISKGRYLEFE SITKERVKKG YRILKLDELI RTRRTVKEAR 400
    FLAAVKECGI HAPSIYDIDK ENKKIIMGYI HGKIAKDEIE EGNIEFCKSL 450
    GETIGKMHGS GIVHNDLTTS NFIISKNPYI IDFGLGKYSD LIEDKAIDLI 500
    VLKKSIMSIH YDKFDLVWNK IIDGYKTYEL AESVLECIKE VEKRARYL 548
    Length:548
    Mass (Da):61,248
    Last modified:July 5, 2004 - v1
    Checksum:iF3EB6F358157768B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF29971.1.
    RefSeqiNP_987535.1. NC_005791.1.
    WP_011170359.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF29971; CAF29971; MMP0415.
    GeneIDi2762045.
    KEGGimmp:MMP0415.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF29971.1 .
    RefSeqi NP_987535.1. NC_005791.1.
    WP_011170359.1. NC_005791.1.

    3D structure databases

    ProteinModelPortali Q6M056.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267377.MMP0415.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF29971 ; CAF29971 ; MMP0415 .
    GeneIDi 2762045.
    KEGGi mmp:MMP0415.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109569.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci MMAR267377:GJ77-442-MONOMER.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.

    Entry informationi

    Entry nameiKAE1B_METMP
    AccessioniPrimary (citable) accession number: Q6M056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3