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Protein

2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

Gene

aroA'

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH) (Probable). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids and which is also a precursor for the biosynthesis of p-aminobenzoic acid (PABA) in M.maripaludis. Does not possess fructose-bisphosphate (FBP) aldolase activity.UniRule annotationCurated1 Publication

Catalytic activityi

L-aspartate 4-semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate = 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + 2,3-dioxopropylaldehyde phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331Proton acceptorUniRule annotation
Active sitei153 – 1531Proton donorUniRule annotation
Active sitei184 – 1841Schiff-base intermediate with substrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthaseUniRule annotation (EC:2.2.1.10UniRule annotation)
Short name:
ADH synthaseUniRule annotation
Short name:
ADHSUniRule annotation
Short name:
ADTH synthaseUniRule annotation
Gene namesi
Name:aroA'UniRule annotation
Ordered Locus Names:MMP0686
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000590 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene require both aromatic amino acids (AroAAs) and p-aminobenzoic acid (PABA) for growth. In the presence of PABA and AroAAs, growth of the mutant strain laggs about 30 hours behind that of wild-type strain. By complementing the mutant strain with the expression of aroA', the strain grows in minimal medium with acetate only. Aryl acids do not replace the requirement of AroAAs for the growth of the deletion mutant strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2722722-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthasePRO_0000363669Add
BLAST

Interactioni

Subunit structurei

Homodecamer.UniRule annotation

Protein-protein interaction databases

STRINGi267377.MMP0686.

Structurei

3D structure databases

ProteinModelPortaliQ6LZE3.
SMRiQ6LZE3. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 375DKFP bindingUniRule annotation
Regioni153 – 1553DKFP bindingUniRule annotation
Regioni209 – 2102DKFP bindingUniRule annotation
Regioni237 – 2382DKFP bindingUniRule annotation

Sequence similaritiesi

Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04044. Archaea.
COG1830. LUCA.
HOGENOMiHOG000224790.
KOiK16306.
OMAiIVRHGHR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00960. ADH_synthase. 1 hit.
InterProiIPR010210. ADH_synthase.
IPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]
PfamiPF01791. DeoC. 1 hit.
[Graphical view]
PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.
SMARTiSM01133. DeoC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01949. AroFGH_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6LZE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMFDNIKNV GKLIRLERIF DKKSEKTVII PMDHGVSSGP LDGIKDMRIT
60 70 80 90 100
TNAVADGGAN AVLGHKGLVR HGHRGYGRDI GLIIHMSAGT SISPDPNKKV
110 120 130 140 150
IVTTVEDAMR MGADAVSLHV NVGAESDFEM YRDLGLISET CEHWGMPLIA
160 170 180 190 200
MMYPRGPKIK DEKDPEVVAH AARLGAELGA DIIKTNYTGD PDTFKEVVKG
210 220 230 240 250
CPAPIVIAGG PKTNTDEEFL QMVKDAMHAG GKGVASGRNV FQHKDVKGIT
260 270
SAICKIVHED VEVEEALKEI KI
Length:272
Mass (Da):29,418
Last modified:July 5, 2004 - v1
Checksum:i5102BCB8D68514EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF30242.1.
RefSeqiWP_011170630.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF30242; CAF30242; MMP0686.
GeneIDi2761335.
KEGGimmp:MMP0686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF30242.1.
RefSeqiWP_011170630.1. NC_005791.1.

3D structure databases

ProteinModelPortaliQ6LZE3.
SMRiQ6LZE3. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267377.MMP0686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF30242; CAF30242; MMP0686.
GeneIDi2761335.
KEGGimmp:MMP0686.

Phylogenomic databases

eggNOGiarCOG04044. Archaea.
COG1830. LUCA.
HOGENOMiHOG000224790.
KOiK16306.
OMAiIVRHGHR.

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-714-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00960. ADH_synthase. 1 hit.
InterProiIPR010210. ADH_synthase.
IPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]
PfamiPF01791. DeoC. 1 hit.
[Graphical view]
PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.
SMARTiSM01133. DeoC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01949. AroFGH_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADHS_METMP
AccessioniPrimary (citable) accession number: Q6LZE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.