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Q6LZD3 (SYP_METMP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Proline--tRNA ligase
EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:MMP0696
OrganismMethanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]
Taxonomic identifier267377 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571

Subunit structure

Homodimer By similarity. HAMAP MF_01571

Subcellular location

Cytoplasm By similarity HAMAP MF_01571.

Domain

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

proline-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Proline--tRNA ligase HAMAP MF_01571
PRO_0000249158

Experimental info

Sequence conflict3041N → K in AAG28517. Ref.1
Sequence conflict3801E → D in AAG28517. Ref.1
Sequence conflict3841L → I in AAG28517. Ref.1
Sequence conflict3901V → I in AAG28517. Ref.1
Sequence conflict3971L → V in AAG28517. Ref.1
Sequence conflict4011N → S in AAG28517. Ref.1
Sequence conflict4051S → A in AAG28517. Ref.1
Sequence conflict4131K → E in AAG28517. Ref.1
Sequence conflict4261N → E in AAG28517. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6LZD3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: DFD7C169F00980F7

FASTA46053,319
        10         20         30         40         50         60 
MEFSEWYSDI LEKAGIYDLR YPIKGCGVYL PYGFKIRRYS FEILRKLLDE TGHDETLFPM 

        70         80         90        100        110        120 
LIPENLLAKE GEHIKGFEDE VFWVTHGGKT PLEVKLALRP TSETTMYYMM KQWIKVHTDL 

       130        140        150        160        170        180 
PLKLYQVVNT FRYETKHTRP LIRLREIMSF KEAHTAHATK KECDAQIEEA LTLYKAFFDE 

       190        200        210        220        230        240 
IGVPYVISKR PEWDKFPGAD YTMAFDTIYP DGKTMQIGTV HNLGQNFAKT FELEFETPDG 

       250        260        270        280        290        300 
EKDFVYQTCY GISDRAIASL ISVHGDEKGL VIPVDVAPIQ IVLIPLLFKG KEEIVMDKIK 

       310        320        330        340        350        360 
ELNNTLKSEF RVHLDDRDIR PGRKYNDWEL KGVPLRIELG PRDIENGHAL IVRRDTGEKI 

       370        380        390        400        410        420 
TVEYSNILEE VEKIVSMYKE NLKLKAEEKV KSFITVLDFE NDVNSLSEKV KAKLLENKGI 

       430        440        450        460 
ILIPFNESIY NEEFEELIDA SVLGLTTYEG KEYISVARTY

« Hide

References

« Hide 'large scale' references
[1]"A single class II synthetase specifies two amino acids in archaeal translation."
Stathopoulos C., Li T., Longman R., Vothknecht U.C., Becker H.D., Ibba M., Soell D.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis."
Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. expand/collapse author list , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A.
J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S2 / LL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF197899 Genomic DNA. Translation: AAG28517.1.
BX950229 Genomic DNA. Translation: CAF30252.1.
RefSeqNP_987816.1. NC_005791.1.

3D structure databases

HSSPHSSP built from PDB template 1NJ8 based on UniProtKB Q58635.
ProteinModelPortalQ6LZD3.
SMRQ6LZD3. Positions 1-460.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2762205.
GenomeReviewsGene locus MMP0696 in contig BX950229_GR.
KEGGmmp:MMP0696.
NMPDRfig|267377.1.peg.696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG334108.
OMAKFAEYEL.
ProtClustDBPRK08661.

Enzyme and pathway databases

BioCycMMAR267377:MMP0696-MONOMER.

Family and domain databases

HAMAPMF_01571. Pro_tRNA_synth_type3.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-synth_IIa.
IPR004499. Pro-tRNA-synth_IIa_arc-type.
IPR017449. Pro-tRNA_synth_II.
IPR015264. Pro-tRNA_synth_II_arc.
IPR016061. Pro-tRNA_synth_II_C.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KOK01881.
PANTHERPTHR11451:SF6. ProS_fam_I. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF09181. ProRS-C_2. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMsTIGR00408. ProS_fam_I. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_METMP
AccessionPrimary (citable) accession number: Q6LZD3
Secondary accession number(s): Q9HHB1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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