Reviewed, UniProtKB/Swiss-Prot Q6LZ92 (ASPD_METMP)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	MMP0737

Organism

Methanococcus maripaludis [Complete proteome] [HAMAP]

Taxonomic identifier

39152 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_0000144899
Sites
	Active site	218	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	190	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LZ92-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 061F6D2566E0E33A	FASTA	267	28,593
	10 20 30 40 50 60 MLKIGLVGCG AIASLITKAL MSDRLNKAEV LAFYDGNLEK AEKLAMETGA DFCKSLDELV 70 80 90 100 110 120 SKDLDLIVEC ASVNAVEDTV IKSLNNDKDV IIMSVGAFAD KDLFVKLYKL AEKLGKKIYV 130 140 150 160 170 180 PSGAVAGIDA VKSGSLGKIS EVSLTTTKPV HGLKSALEEQ GLNTDEIKEP KIVFEGTVFD 190 200 210 220 230 240 AISKFPQNIN VSVVLSLASR YPAKVKIIAD PNAVVNRHEI LVKGSIGTIK TCVENNPCRD 250 260 NPKTSALAAY SVIRLIKDLS EPVRIGT

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950229 Genomic DNA. Translation: CAF30293.1.
RefSeq	NP_987857.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2761445.
GenomeReviews	Gene locus MMP0737 in contig BX950229_GR.
KEGG	mmp:MMP0737.
NMPDR	fig|267377.1.peg.737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LZ92.
OMA	ECAGHSA.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0737-MON.
BRENDA	1.4.1.21. 20956.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_METMP

Accession

Primary (citable) accession number: Q6LZ92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)