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Q6LX78

- SYI_METMP

UniProt

Q6LX78 - SYI_METMP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei601 – 6011ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-1512-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MMP1474
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10341034Isoleucine--tRNA ligasePRO_0000098581Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267377.MMP1474.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LX78.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi46 – 5611"HIGH" regionAdd
    BLAST
    Motifi598 – 6025"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6LX78-1 [UniParc]FASTAAdd to Basket

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    MKQVKSVNFR ELDKKVKEYW KKENTYKKVK ALNEHGPEYY FVDGPPYCSG     50
    AIHLGTAWNK IIKDTVLRFK RIQGYNVLDK AGWDMHGLPI EVKVENEFNI 100
    GSKKDIETKI GTQEFINKCK EFALNHLGHM QGQFENLGVW LDFENAYMPI 150
    KRDYMEMGWW TLKKAHEKEL LTKDLRSGYW CPRCETSLAE HEVRGEYKEV 200
    LDPSVYVKFK LEKSDEYITI WTTTPWTLPS NMLVCVNPEF DYAYVNVEFE 250
    NGTAETWIIA EKLVNDVMKK AEKNNDISKF SISKVVKGDS LIGLKYIHPL 300
    LEENEKQQEF AKIENVHTIV PGDHVTLEGG TGLVHTAPGF GEDDFNIGKE 350
    HNIPVYAPID DNGKYTDSIW KGTFVKDMDE SVIETLISKN LLVNSGKVKH 400
    TYPHCWRCKT PLLFRATEQW FLSISKIKDS IIEQGKTVDW VPDWVKTRYV 450
    NGVSFVGDWN ISRQRYWGIP LPIWICEECG NYEVIGSVDE LKERANEKDV 500
    DLSDIHKPAV DKITLTCSCG GKMKRTPDVL DVWYDSGLAP YASIGSKKLK 550
    KAQFITEGND QVTKWFYSQH ALSAVVFDDT SYEKCMMHGF TLDETGEKMS 600
    KSLGNIVSPD DVTEQYGADV LRFYLLSANK AWEDLRFSYS EMDETRSMLN 650
    TLWNSYAFSA NYMVLDDFVP NNEYFKHVKD EDAWILSRIN TVAKEAVEAL 700
    EKPHLHVYTW ALRDFILNDF SRWYIKLIRD RTWMEKNDVQ KLSAYQTLYY 750
    VIMKLISIMA PVTPHLSEEI YQNLKTEDMP ESIFMNKLTI ESEFINETLE 800
    KDTEIIREIV DSILKGRDKA KYTLRYPITK ITLPENIAET VEKYGYIIKE 850
    QGNVKEIELK EFEGNITVKP NFKELGKIFR SDVPKVVAAI NSVAPNELKE 900
    KLKSGNFEVS EYEIKPEYVE FRVEIPENIV GVEFSKGNVY INIEMNDEVI 950
    KEGLVREVVR RIQSMRKDMD LDINEKINVK LEGIDFSSDY LSHIANEVRG 1000
    NFVESLKSDY NQKWTIKTPN EETYDISIDI EKNK 1034
    Length:1,034
    Mass (Da):119,910
    Last modified:July 5, 2004 - v1
    Checksum:iA9C573A4C0E3ECB8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF31030.1.
    RefSeqiNP_988594.1. NC_005791.1.
    WP_011171418.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF31030; CAF31030; MMP1474.
    GeneIDi2761573.
    KEGGimmp:MMP1474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF31030.1 .
    RefSeqi NP_988594.1. NC_005791.1.
    WP_011171418.1. NC_005791.1.

    3D structure databases

    ProteinModelPortali Q6LX78.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267377.MMP1474.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF31030 ; CAF31030 ; MMP1474 .
    GeneIDi 2761573.
    KEGGi mmp:MMP1474.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MMAR267377:GJ77-1512-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.

    Entry informationi

    Entry nameiSYI_METMP
    AccessioniPrimary (citable) accession number: Q6LX78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3