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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei601 – 6011ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-1512-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MMP1474
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000590 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Isoleucine--tRNA ligasePRO_0000098581Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267377.MMP1474.

Structurei

3D structure databases

ProteinModelPortaliQ6LX78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi598 – 6025"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LX78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQVKSVNFR ELDKKVKEYW KKENTYKKVK ALNEHGPEYY FVDGPPYCSG
60 70 80 90 100
AIHLGTAWNK IIKDTVLRFK RIQGYNVLDK AGWDMHGLPI EVKVENEFNI
110 120 130 140 150
GSKKDIETKI GTQEFINKCK EFALNHLGHM QGQFENLGVW LDFENAYMPI
160 170 180 190 200
KRDYMEMGWW TLKKAHEKEL LTKDLRSGYW CPRCETSLAE HEVRGEYKEV
210 220 230 240 250
LDPSVYVKFK LEKSDEYITI WTTTPWTLPS NMLVCVNPEF DYAYVNVEFE
260 270 280 290 300
NGTAETWIIA EKLVNDVMKK AEKNNDISKF SISKVVKGDS LIGLKYIHPL
310 320 330 340 350
LEENEKQQEF AKIENVHTIV PGDHVTLEGG TGLVHTAPGF GEDDFNIGKE
360 370 380 390 400
HNIPVYAPID DNGKYTDSIW KGTFVKDMDE SVIETLISKN LLVNSGKVKH
410 420 430 440 450
TYPHCWRCKT PLLFRATEQW FLSISKIKDS IIEQGKTVDW VPDWVKTRYV
460 470 480 490 500
NGVSFVGDWN ISRQRYWGIP LPIWICEECG NYEVIGSVDE LKERANEKDV
510 520 530 540 550
DLSDIHKPAV DKITLTCSCG GKMKRTPDVL DVWYDSGLAP YASIGSKKLK
560 570 580 590 600
KAQFITEGND QVTKWFYSQH ALSAVVFDDT SYEKCMMHGF TLDETGEKMS
610 620 630 640 650
KSLGNIVSPD DVTEQYGADV LRFYLLSANK AWEDLRFSYS EMDETRSMLN
660 670 680 690 700
TLWNSYAFSA NYMVLDDFVP NNEYFKHVKD EDAWILSRIN TVAKEAVEAL
710 720 730 740 750
EKPHLHVYTW ALRDFILNDF SRWYIKLIRD RTWMEKNDVQ KLSAYQTLYY
760 770 780 790 800
VIMKLISIMA PVTPHLSEEI YQNLKTEDMP ESIFMNKLTI ESEFINETLE
810 820 830 840 850
KDTEIIREIV DSILKGRDKA KYTLRYPITK ITLPENIAET VEKYGYIIKE
860 870 880 890 900
QGNVKEIELK EFEGNITVKP NFKELGKIFR SDVPKVVAAI NSVAPNELKE
910 920 930 940 950
KLKSGNFEVS EYEIKPEYVE FRVEIPENIV GVEFSKGNVY INIEMNDEVI
960 970 980 990 1000
KEGLVREVVR RIQSMRKDMD LDINEKINVK LEGIDFSSDY LSHIANEVRG
1010 1020 1030
NFVESLKSDY NQKWTIKTPN EETYDISIDI EKNK
Length:1,034
Mass (Da):119,910
Last modified:July 5, 2004 - v1
Checksum:iA9C573A4C0E3ECB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF31030.1.
RefSeqiNP_988594.1. NC_005791.1.
WP_011171418.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF31030; CAF31030; MMP1474.
GeneIDi2761573.
KEGGimmp:MMP1474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF31030.1.
RefSeqiNP_988594.1. NC_005791.1.
WP_011171418.1. NC_005791.1.

3D structure databases

ProteinModelPortaliQ6LX78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267377.MMP1474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF31030; CAF31030; MMP1474.
GeneIDi2761573.
KEGGimmp:MMP1474.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-1512-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S2 / LL.

Entry informationi

Entry nameiSYI_METMP
AccessioniPrimary (citable) accession number: Q6LX78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: April 29, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.