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Q6LX63 (NPPNK_METMP) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional NADP phosphatase/NAD kinase

Including the following 2 domains:

  1. NAD kinase
    EC=2.7.1.23
    Alternative name(s):
    ATP-dependent NAD kinase
    Poly(P)-dependent NAD kinase
    Short name=PPNK
  2. NADP phosphatase
    Short name=NADPase
    Short name=pNPPase
    EC=3.1.3.-
Gene names
Ordered Locus Names:MMP1489
OrganismMethanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]
Taxonomic identifier267377 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes the phosphorylation and dephosphorylation of NAD and NADP, respectively. Although it shows conflicting dual activities and is able to supply NADP, it seems that its physiological role is to prevent excess accumulation of NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

NADP+ + H2O = NAD+ + phosphate. HAMAP-Rule MF_00361

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00361

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

In the N-terminal section; belongs to the inositol monophosphatase family.

In the C-terminal section; belongs to the NAD kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Bifunctional NADP phosphatase/NAD kinase HAMAP-Rule MF_00361
PRO_0000229718

Regions

Nucleotide binding355 – 3562NAD By similarity
Nucleotide binding430 – 4312NAD By similarity
Nucleotide binding471 – 4766NAD By similarity
Region1 – 283283NADP phosphatase HAMAP-Rule MF_00361
Region275 – 566292NAD kinase HAMAP-Rule MF_00361

Sites

Active site3551Proton acceptor By similarity
Metal binding661Magnesium 1 By similarity
Metal binding851Magnesium 1 By similarity
Metal binding851Magnesium 2 By similarity
Metal binding881Magnesium 2 By similarity
Metal binding2291Magnesium 2 By similarity
Binding site881Substrate By similarity
Binding site3601NAD By similarity
Binding site4411NAD By similarity
Binding site4581NAD By similarity
Binding site4601NAD By similarity
Binding site4681NAD; via carbonyl oxygen By similarity
Binding site5281NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6LX63 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1BE5DAC1288E60B0

FASTA56662,594
        10         20         30         40         50         60 
MDMLEMALNI AKDIEKSVKP LIGWEKSNEV VKIGADGTPT KRIDLIAENV AINSIEKVCS 

        70         80         90        100        110        120 
AILISEEIGF KRIGKNKPEY VIVLDPVDGT YNSLKDIPFY SAAVAIGRID KFADNLEELI 

       130        140        150        160        170        180 
NNLKMKDLEV GVVRNIATGD TYYAEKGKGA HFLRKGEKKS ISISNSSNLK DSSIGLFAHD 

       190        200        210        220        230        240 
ISIDTLKFIK DRRFRRIRLF GSIALEMCYV AKGALDAFIN VNETTRLCDI AAGYVIIKEA 

       250        260        270        280        290        300 
GGIVTDKNGQ EVNLDLDVNS KVSVICSNEM LHKKLVGIFG NRWRIKPTNF GIISRIDNEE 

       310        320        330        340        350        360 
SIEVADNVIK YLDSKGIKYE LDSSTYNALK NRLTKKCDII SNIEEISHMI SIGGDGTVLR 

       370        380        390        400        410        420 
ASKMIEGNEI PMVCINMGTV GFLTEFNKDE IFSAIDSIIC GSYKVEKRTK LMGFAKLSDG 

       430        440        450        460        470        480 
KQHILNDSLN EVVITTKNPA KMMHFEVYID GSLVEDVRAD GIIVSTPNGS TAYSLSSGGP 

       490        500        510        520        530        540 
IIEPTVEGFV IVPICPFKLS SRPLVVNANS EIKIKLLKKS TYVVIDGNTE FEAKKGDEII 

       550        560 
LRKSESNAYF VKGDNFYNKL KKLSLM 

« Hide

References

[1]"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis."
Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. expand/collapse author list , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A.
J. Bacteriol. 186:6956-6969(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S2 / LL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950229 Genomic DNA. Translation: CAF31045.1.
RefSeqNP_988609.1. NC_005791.1.

3D structure databases

ProteinModelPortalQ6LX63.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267377.MMP1489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAF31045; CAF31045; MMP1489.
GeneID2761729.
KEGGmmp:MMP1489.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000253998.
KOK00858.
OMAVIVPICP.

Enzyme and pathway databases

BioCycMMAR267377:GJ77-1527-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR021175. Bifunctional_PpnK_predicted.
IPR000760. Inositol_monophosphatase.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF00459. Inositol_P. 1 hit.
PF01513. NAD_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF036641. Bifunctional_PpnK_predicted. 1 hit.
PRINTSPR00377. IMPHPHTASES.
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNPPNK_METMP
AccessionPrimary (citable) accession number: Q6LX63
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families