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Q6LX63

- NPPNK_METMP

UniProt

Q6LX63 - NPPNK_METMP

Protein

Bifunctional NADP phosphatase/NAD kinase

Gene

MMP1489

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes the phosphorylation and dephosphorylation of NAD and NADP, respectively. Although it shows conflicting dual activities and is able to supply NADP, it seems that its physiological role is to prevent excess accumulation of NADP By similarity.By similarity

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation
    NADP+ + H2O = NAD+ + phosphate.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Magnesium 1By similarity
    Metal bindingi85 – 851Magnesium 1By similarity
    Metal bindingi85 – 851Magnesium 2By similarity
    Metal bindingi88 – 881Magnesium 2By similarity
    Binding sitei88 – 881SubstrateBy similarity
    Metal bindingi229 – 2291Magnesium 2By similarity
    Active sitei355 – 3551Proton acceptorUniRule annotation
    Binding sitei360 – 3601NADUniRule annotation
    Binding sitei441 – 4411NADUniRule annotation
    Binding sitei458 – 4581NADUniRule annotation
    Binding sitei460 – 4601NADUniRule annotation
    Binding sitei468 – 4681NAD; via carbonyl oxygenUniRule annotation
    Binding sitei528 – 5281NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi355 – 3562NADUniRule annotation
    Nucleotide bindingi430 – 4312NADUniRule annotation
    Nucleotide bindingi471 – 4766NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. NAD+ kinase activity Source: UniProtKB
    4. NAD binding Source: UniProtKB
    5. NADP phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. lysine metabolic process Source: InterPro
    3. NAD metabolic process Source: InterPro
    4. NADP biosynthetic process Source: InterPro
    5. phosphatidylinositol phosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-1527-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional NADP phosphatase/NAD kinase
    Including the following 2 domains:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Poly(P)-dependent NAD kinase
    Short name:
    PPNK
    NADP phosphatase (EC:3.1.3.-)
    Short name:
    NADPase
    Short name:
    pNPPase
    Gene namesi
    Ordered Locus Names:MMP1489
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566Bifunctional NADP phosphatase/NAD kinasePRO_0000229718Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi267377.MMP1489.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LX63.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 283283NADP phosphataseAdd
    BLAST
    Regioni275 – 566292NAD kinaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the inositol monophosphatase family.Curated
    In the C-terminal section; belongs to the NAD kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000253998.
    KOiK00858.
    OMAiVIVPICP.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR021175. Bifunctional_PpnK_predicted.
    IPR000760. Inositol_monophosphatase.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    PF01513. NAD_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036641. Bifunctional_PpnK_predicted. 1 hit.
    PRINTSiPR00377. IMPHPHTASES.
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6LX63-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDMLEMALNI AKDIEKSVKP LIGWEKSNEV VKIGADGTPT KRIDLIAENV    50
    AINSIEKVCS AILISEEIGF KRIGKNKPEY VIVLDPVDGT YNSLKDIPFY 100
    SAAVAIGRID KFADNLEELI NNLKMKDLEV GVVRNIATGD TYYAEKGKGA 150
    HFLRKGEKKS ISISNSSNLK DSSIGLFAHD ISIDTLKFIK DRRFRRIRLF 200
    GSIALEMCYV AKGALDAFIN VNETTRLCDI AAGYVIIKEA GGIVTDKNGQ 250
    EVNLDLDVNS KVSVICSNEM LHKKLVGIFG NRWRIKPTNF GIISRIDNEE 300
    SIEVADNVIK YLDSKGIKYE LDSSTYNALK NRLTKKCDII SNIEEISHMI 350
    SIGGDGTVLR ASKMIEGNEI PMVCINMGTV GFLTEFNKDE IFSAIDSIIC 400
    GSYKVEKRTK LMGFAKLSDG KQHILNDSLN EVVITTKNPA KMMHFEVYID 450
    GSLVEDVRAD GIIVSTPNGS TAYSLSSGGP IIEPTVEGFV IVPICPFKLS 500
    SRPLVVNANS EIKIKLLKKS TYVVIDGNTE FEAKKGDEII LRKSESNAYF 550
    VKGDNFYNKL KKLSLM 566
    Length:566
    Mass (Da):62,594
    Last modified:July 5, 2004 - v1
    Checksum:i1BE5DAC1288E60B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF31045.1.
    RefSeqiNP_988609.1. NC_005791.1.
    WP_011171433.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF31045; CAF31045; MMP1489.
    GeneIDi2761729.
    KEGGimmp:MMP1489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF31045.1 .
    RefSeqi NP_988609.1. NC_005791.1.
    WP_011171433.1. NC_005791.1.

    3D structure databases

    ProteinModelPortali Q6LX63.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267377.MMP1489.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF31045 ; CAF31045 ; MMP1489 .
    GeneIDi 2761729.
    KEGGi mmp:MMP1489.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000253998.
    KOi K00858.
    OMAi VIVPICP.

    Enzyme and pathway databases

    BioCyci MMAR267377:GJ77-1527-MONOMER.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR021175. Bifunctional_PpnK_predicted.
    IPR000760. Inositol_monophosphatase.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF00459. Inositol_P. 1 hit.
    PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036641. Bifunctional_PpnK_predicted. 1 hit.
    PRINTSi PR00377. IMPHPHTASES.
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.

    Entry informationi

    Entry nameiNPPNK_METMP
    AccessioniPrimary (citable) accession number: Q6LX63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3