Reviewed, UniProtKB/Swiss-Prot Q6LWL2 (HDRA_METMP)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase iron-sulfur subunit A
    EC=1.8.98.1

Gene names

Name:	hdrA
Ordered Locus Names:	MMP1697

Organism

Methanococcus maripaludis [Complete proteome] [HAMAP]

Taxonomic identifier

39152 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). May act as the catalytic subunit By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 4 4Fe-4S clusters per subunit By similarity. FAD By similarity.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 1 is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrA family. Contains 4 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Methanogenesis
Coding sequence diversity	Selenocysteine
Domain	Repeat
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding Selenium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	CoB--CoM heterodisulfide reductase iron-sulfur subunit A		PRO_0000318646
Regions
	Domain	236 – 267	32	4Fe-4S ferredoxin-type 1
	Domain	284 – 313	30	4Fe-4S ferredoxin-type 2
	Domain	575 – 604	30	4Fe-4S ferredoxin-type 3
	Domain	607 – 637	31	4Fe-4S ferredoxin-type 4
	Nucleotide binding	150 – 173	24	FAD Potential
Sites
	Metal binding	246	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	249	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	252	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	256	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	293	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	296	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	299	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	303	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	584	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	587	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	590	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	594	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	617	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	620	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	623	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	627	1	Iron-sulfur 3 (4Fe-4S) Potential
Amino acid modifications
	Non-standard residue	197	1	Selenocysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q6LWL2-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 0218716DA82E3866	FASTA	658	71,281
	10 20 30 40 50 60 MSDPKVGVFV CYCGANINGA VDCEAVKDFA SELDGVAVAA TYPFMCADPG QGLIKDAIKE 70 80 90 100 110 120 HGLDRIVVAA CTPKIHEPTF RGCLQDAGIS PYYLEFVNIR EHDAFVHMGD VEGATRKACE 130 140 150 160 170 180 MIAGGVERAK KLEDVPQKVV DVDKSCMVIG AGIAGIQSAL DLGDQGFKVY LVDKDESIGG 190 200 210 220 230 240 RMAQLAKTFP TDDCAMUILA PKMVSAANHP NIELITFAEI KNIDGYIGNF DVTLEKKPRY 250 260 270 280 290 300 VDEDTCTGCG ACAAACPIEV PNEFDLGLGT RKAIYVPFPQ AVPLLYTIDK EHCIDCGLCA 310 320 330 340 350 360 KVCCAEAVRY DQKPQELNIK VGTIITATGY DEFDATKKEE YGYGVYDNVI TTLEVERMIN 370 380 390 400 410 420 PAGPTHGHEI RPSDGKAPKR TVYIQCVGSR DEKVGNPYCS RVCCMFALKN AQLMKMHDPN 430 440 450 460 470 480 AEVYICYMDI RAFGKGYEEY YKRAQDQFGV KFIRGRPANI FEDPETKNLT VRVEDTLMGE 490 500 510 520 530 540 ILEIDADLVV LSAGLEAKKD AGELAKMLGI DRGPEGFFKE LHPKLAPVNT KVDGIAIAGV 550 560 570 580 590 600 AQGPKDIPDT VAQAKGAASA VAIPMSQGQF KIEMIRATVN EEVCGGCKVC ALMCPYNAIT 610 620 630 640 650 YEEKDGHLVA ITDDVACKGC GACAAACPSG AMQLRYYRDE QVIGMIDGIL NAAKMLEE

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950229 Genomic DNA. Translation: CAF31253.1.
RefSeq	NP_988817.1.
3D structure databases
HSSP	HSSP built from PDB template 1BD6 based on UniProtKB Q45560.
ModBase	Search...
Genome annotation databases
GeneID	2762636.
GenomeReviews	Gene locus MMP1697 in contig BX950229_GR.
KEGG	mmp:MMP1697.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LWL2.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00037. Fer4. 4 hits. PF00070. Pyr_redox. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
PROSITE	PS00198. 4FE4S_FER_1. 3 hits. PS51379. 4FE4S_FER_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRA_METMP

Accession

Primary (citable) accession number: Q6LWL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	November 25, 2008
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)