ID   FADB_PHOPR              Reviewed;         720 AA.
AC   Q6LW06;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.3.3.8;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadB; OrderedLocusNames=PBPRA0064;
OS   Photobacterium profundum (Photobacterium sp. (strain SS9)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Photobacterium.
OX   NCBI_TaxID=74109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15746425; DOI=10.1126/science.1103341;
RA   Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N.,
RA   Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N.,
RA   Romualdi C., Bartlett D.H., Valle G.;
RT   "Life at depth: Photobacterium profundum genome sequence and
RT   expression analysis.";
RL   Science 307:1459-1461(2005).
CC   -!- FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by
CC       addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA
CC       epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-
CC       CoA.
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta
CC       chains (fadA) (By similarity).
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the 3-
CC       hydroxyacyl-CoA dehydrogenase family.
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DR   EMBL; CR378663; CAG18519.1; -; Genomic_DNA.
DR   RefSeq; YP_128321.1; -.
DR   GeneID; 3123934; -.
DR   GenomeReviews; CR354531_GR; PBPRA0064.
DR   KEGG; ppr:PBPRA0064; -.
DR   NMPDR; fig|298386.1.peg.2294; -.
DR   HOGENOM; Q6LW06; -.
DR   OMA; Q6LW06; ANNGSYY.
DR   BioCyc; PPRO298386:PBPRA0064-MON; -.
DR   BRENDA; 1.1.1.35; 263915.
DR   BRENDA; 4.2.1.17; 263915.
DR   BRENDA; 5.1.2.3; 263915.
DR   BRENDA; 5.3.3.8; 263915.
DR   GO; GO:0016507; C:fatty acid beta-oxidation multienzyme complex; IEA:InterPro.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:HAMAP.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
DR   GO; GO:0009062; P:fatty acid catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01621; -; 1.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR012799; FadB.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   TIGRFAMs; TIGR02437; FadB; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Fatty acid metabolism; Isomerase;
KW   Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    720       Fatty acid oxidation complex subunit
FT                                alpha.
FT                                /FTId=PRO_0000109272.
FT   REGION        1    189       Enoyl-CoA hydratase/isomerase (By
FT                                similarity).
FT   REGION      311    720       3-hydroxyacyl-CoA dehydrogenase (By
FT                                similarity).
SQ   SEQUENCE   720 AA;  77647 MW;  859AC731FF016A06 CRC64;
     MIYQGETLSV RYLDDGIAEL NLNAPGAVNK FDLKTLECLN EALNALYQQS DLKGLLITSD
     KDAFIVGADI TEFLGLFAKP AEELSQWLTR ANDIFNKLED LPVPTLSAIN GHALGGGCEC
     VLATDFRLAD ITARIGLPET RLGIMPGFGG TVRLPRLLGA DSAMEIITAG KDKKAQDALK
     LGLVDAVVAP TALKDAALSM IKDAIAGKLD WQKRRAQKKA PLTLNKIEAT MSFTMAKAMV
     AQVAGKHYPA PMTAVIAIEA AARMSRDEAL VVENKHFITL AKTDVAQSLV GIFLNDQYIK
     GKAKKAAKEG QPTKKGVVLG AGIMGGGIAY QSALKGVPVL MKDIAVPSLD LGMAEAAKLL
     NKQLERGRID GVKMAKVLSG ITPSLHYAGA EDADIVVEAV VENPKIKAAV LAEVESNVSD
     TTVIASNTST IPINLLAQSL KRPENFCGMH FFNPVHRMPL VEIIRGEHTS EETISRVVAY
     AAKMGKSPIV VNDCPGFFVN RVLFPYFAGF SLLLRDGADF TQVDKVMEKQ FGWPMGPAYL
     LDVVGIDTAH HAQAVMAEGF PDRMGKNYKD AVDVMFEQQR FGQKNGNGFF AYSVDRRGKP
     KKNVDPAVAE LLAPVLGAAT DFTSDEIIAR MMIPMINEVV RCLEECIIAT PAEADMALVY
     GLGFPPFRGG VFRYIDTLGL AEYVAMADKF AHLGAVYEVP TGLREKAAKG ESYYTQQVNA
//