Reviewed, UniProtKB/Swiss-Prot Q6LW06 (FADB_PHOPR)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.3.3.8
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadB
Ordered Locus Names:	PBPRA0064

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01621 (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01621 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01621 (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. HAMAP MF_01621
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01621
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: HAMAP enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Fatty acid oxidation complex subunit alpha HAMAP MF_01621		PRO_0000109272
Regions
	Region	1 – 189	189	Enoyl-CoA hydratase/isomerase By similarity
	Region	311 – 720	410	3-hydroxyacyl-CoA dehydrogenase By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LW06-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 859AC731FF016A06	FASTA	720	77,647
	10 20 30 40 50 60 MIYQGETLSV RYLDDGIAEL NLNAPGAVNK FDLKTLECLN EALNALYQQS DLKGLLITSD 70 80 90 100 110 120 KDAFIVGADI TEFLGLFAKP AEELSQWLTR ANDIFNKLED LPVPTLSAIN GHALGGGCEC 130 140 150 160 170 180 VLATDFRLAD ITARIGLPET RLGIMPGFGG TVRLPRLLGA DSAMEIITAG KDKKAQDALK 190 200 210 220 230 240 LGLVDAVVAP TALKDAALSM IKDAIAGKLD WQKRRAQKKA PLTLNKIEAT MSFTMAKAMV 250 260 270 280 290 300 AQVAGKHYPA PMTAVIAIEA AARMSRDEAL VVENKHFITL AKTDVAQSLV GIFLNDQYIK 310 320 330 340 350 360 GKAKKAAKEG QPTKKGVVLG AGIMGGGIAY QSALKGVPVL MKDIAVPSLD LGMAEAAKLL 370 380 390 400 410 420 NKQLERGRID GVKMAKVLSG ITPSLHYAGA EDADIVVEAV VENPKIKAAV LAEVESNVSD 430 440 450 460 470 480 TTVIASNTST IPINLLAQSL KRPENFCGMH FFNPVHRMPL VEIIRGEHTS EETISRVVAY 490 500 510 520 530 540 AAKMGKSPIV VNDCPGFFVN RVLFPYFAGF SLLLRDGADF TQVDKVMEKQ FGWPMGPAYL 550 560 570 580 590 600 LDVVGIDTAH HAQAVMAEGF PDRMGKNYKD AVDVMFEQQR FGQKNGNGFF AYSVDRRGKP 610 620 630 640 650 660 KKNVDPAVAE LLAPVLGAAT DFTSDEIIAR MMIPMINEVV RCLEECIIAT PAEADMALVY 670 680 690 700 710 720 GLGFPPFRGG VFRYIDTLGL AEYVAMADKF AHLGAVYEVP TGLREKAAKG ESYYTQQVNA

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378663 Genomic DNA. Translation: CAG18519.1.
RefSeq	YP_128321.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3123934.
GenomeReviews	Gene locus PBPRA0064 in contig CR354531_GR.
KEGG	ppr:PBPRA0064.
NMPDR	fig|298386.1.peg.2294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LW06.
OMA	Q6LW06. ANNGSYY.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA0064-MON.
BRENDA	1.1.1.35. 263915. 4.2.1.17. 263915. 5.1.2.3. 263915. 5.3.3.8. 263915.
Family and domain databases
HAMAP	MF_01621. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012799. FadB. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02437. FadB. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADB_PHOPR

Accession

Primary (citable) accession number: Q6LW06

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)