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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Photobacterium profundum (strain SS9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:PBPRA0448
OrganismiPhotobacterium profundum (strain SS9)
Taxonomic identifieri298386 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000000593 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092651 – 957Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST957

Proteomic databases

PRIDEiQ6LUZ6.

Interactioni

Protein-protein interaction databases

STRINGi298386.PBPRA0448.

Structurei

3D structure databases

ProteinModelPortaliQ6LUZ6.
SMRiQ6LUZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 449Adenylyl removaseUniRule annotationAdd BLAST449
Regioni457 – 957Adenylyl transferaseUniRule annotationAdd BLAST501

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q6LUZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQHLERPEL LSIAATKALD KLQQAHPELL AQWPTERQNE LETVIGLSDF
60 70 80 90 100
IASSLVCDGQ LLLWLSEHLD DKEHAEQYRQ QLETKLATTA DDVGLMRQLR
110 120 130 140 150
AFRRQEMVWI AWRDFTNKST LEQSLAHLSQ LAEALIMEAY QWLYNQCCKE
160 170 180 190 200
WGTPTNSAGE AQPMLVLGMG KLGGGELNFS SDIDLIFTYP ENGDTVGGRR
210 220 230 240 250
SMANAQFFTR LGQRLIKALD QPTYDGFCYR VDMRLRPFGD SGPLVMSYAA
260 270 280 290 300
LEDYYQEQGR DWERYAMIKA RVMGRESFTQ YQELRQMLRP FVFRRYIDFS
310 320 330 340 350
AIQALRRMKS MISSEVRRRG LSNNIKLGAG GIREVEFIAQ SFQLIRGGRE
360 370 380 390 400
PSLRGRGLLE TLAAMKQLAL LPEKQIEHLE QGYCFLRKLE NLLQAIDDKQ
410 420 430 440 450
TQTLPDKPLD QIRLAFAMGY ADWQSLFEAI ELHMSAVHLV FDDIIGTDED
460 470 480 490 500
DAGCSVSEQY NEMWTMAKDE DVLLNIMAEL EAPDSANQVS TILGMKAELS
510 520 530 540 550
KRTLGPRGRE VLTRLMPEVL SRIVPRPDAS AVLARVTKLI VRVATRTTYL
560 570 580 590 600
ELLGEHPAAL GQLIRLCAAS PMVAEQLTQY PILLDELLDP QHLYNPTPLD
610 620 630 640 650
QYGDELREYL ARIPEEDMEQ QMEAIRQYKQ AQLLRIAAAD IAGALPLMAV
660 670 680 690 700
SDHLTYLAEA IITAVVNQAW LQMAEKYGEP THLVDRNGKG FGVIGYGKVG
710 720 730 740 750
GWELGYGSDL DVVFLHDCPA DIYTNGKKEI DGRQFYLRLA QRIVHLFSTR
760 770 780 790 800
TSSGVLYEID VRLRPSGASG LLVSTVESFD EYQQKEAWTW EHQALVRARM
810 820 830 840 850
IYGDVPLFDA FSDVRKRILT QPRDTQSLQV DVVSMRHKMR VHLGSKKNGM
860 870 880 890 900
FGLKQDKGGI TDIEFLAQYL VLQHSHTEPY LTRWSDNVRI FDTMAECEVL
910 920 930 940 950
SLSQASALKQ AYCVMRDDIH RLNLLGLPVY VDESQFVTER ETVQQIWQEY

LVPSSDE
Length:957
Mass (Da):108,957
Last modified:July 5, 2004 - v1
Checksum:iE64E8633A48F3D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378664 Genomic DNA. Translation: CAG18879.1.
RefSeqiWP_011217235.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG18879; CAG18879; PBPRA0448.
KEGGippr:PBPRA0448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378664 Genomic DNA. Translation: CAG18879.1.
RefSeqiWP_011217235.1. NC_006370.1.

3D structure databases

ProteinModelPortaliQ6LUZ6.
SMRiQ6LUZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298386.PBPRA0448.

Proteomic databases

PRIDEiQ6LUZ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG18879; CAG18879; PBPRA0448.
KEGGippr:PBPRA0448.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_PHOPR
AccessioniPrimary (citable) accession number: Q6LUZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 5, 2004
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.