Q6LUQ1 (ACEK_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PBPRA0551

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288290
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LUQ1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A4B7FCFC611FCECB	FASTA	575	67,107
	10 20 30 40 50 60 MTAAMEQLVA HTILQGFDAM YGRFLDVTAG AQERFEQQDW SSVHLALKKR ISFYDHHVGL 70 80 90 100 110 120 VTSQIQIMLG ERYANRSFLM AVKSSYENLL LDYPRYEIAE SFFNSVYCRI FEHRNINRDK 130 140 150 160 170 180 LFVHSSQENR IPTYPTQLTR IYHAHSGLTS LFDRVLDDTP FTQVWEDKQR DTKLIINHLK 190 200 210 220 230 240 QELGAAFNDE TSLELIREPF YRNKAAYLIG KITLNHDGQY QTIPLVLPVL NNSNKQLYVD 250 260 270 280 290 300 ACICHVNDAS IIFGFARSYF MVYAPAPGAL VRFLSNLIPN KTNAELYTAI GCQKHGKTEL 310 320 330 340 350 360 YREFLTHLDN SDDQFVTAPG IKGMVMSVFT LPSYDFVFKI IKDKFAPQKD ISHATVKEKY 370 380 390 400 410 420 KLVKEHDRVG RMADTQEYRH FTFERHRFSD ELLAELLAVA PSIIKVTSDQ IIISHLYMER 430 440 450 460 470 480 RMIPFNIYVE QANDDDLRDA VDEYGKAIKQ LAAANIFPGD MLFKNFGVTR HKRVVFYDYD 490 500 510 520 530 540 EISYMTEMNF RKIPEPRTPE DEMSAEPWYS VGIYDVFPEE FRTFLLINPK VKALFNELHS 550 560 570 DLFEAKYWQS LQQNITHGQY EDVYPYRGVH RLRKT

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378664 Genomic DNA. Translation: CAG18974.1.
RefSeq	YP_128776.1. NC_006370.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3122314.
GenomeReviews	Gene locus PBPRA0551 in contig CR354531_GR.
KEGG	ppr:PBPRA0551.
NMPDR	fig|298386.1.peg.2776.
PATRIC	22931788. VBIPhoPro109272_0626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q6LUQ1.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA0551-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PHOPR

Accession

Primary (citable) accession number: Q6LUQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program