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Q6LUL1

- SYI_PHOPR

UniProt

Q6LUL1 - SYI_PHOPR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei572 – 5721Aminoacyl-adenylateUniRule annotation
    Binding sitei616 – 6161ATPUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation
    Metal bindingi937 – 9371ZincUniRule annotation
    Metal bindingi940 – 9401ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PBPRA0591
    OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
    Taxonomic identifieri74109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
    ProteomesiUP000000593: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 954954Isoleucine--tRNA ligasePRO_0000098437Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi298386.PBPRA0591.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LUL1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi613 – 6175"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6LUL1-1 [UniParc]FASTAAdd to Basket

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    MSDYKDTLNL PETGFPMRGN LAQREPVMLK RWDDEDLYGE IRKAKKGNKS    50
    FILHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YIPGWDCHGL 100
    PIELMVEKKW GKPGRKLTAA EFRQKCREYA AGQVEGQKES FIRLGVLGQW 150
    DKPYRTMDFA TEANIIRSLG KVAENDHLLK GFKPVHWCTD CGSALAEAEV 200
    EYQDKVSPSI DVKFKAVDEA GVVAKFNCAE GHEGQGDVSV VIWTTTPWTM 250
    PANRAVAVRD DLEYVLVQVE ADAANDKAAY RLIVAAELAK DVMDRAGIEH 300
    FHNLGFCKGA DLELMRFNHP FYSFDVPIVL GDHVTTESGT GCVHTAPGHG 350
    QEDFVVGQKY GLEIANPVGS NGVYLPDTEL FAGQHVFKAN DVVVETLKEH 400
    GALLHHHAYE HSYPHCWRHK TPIIFRATPQ WFISMDKAGL RAKALGEIKN 450
    VQWLPEWGQS RIEGMVEGRP EWCISRQRTW GVPIALFVHK ETQELHPDTR 500
    VLIEKVAQLV EQKGIQAWWD LNPAELMGEA DAANYEKVLD TLDVWFDSGV 550
    THFSVVDSRE EYNGHSADLY LEGSDQHRGW FQSSLVSSVA MKGKAPYNQV 600
    LTHGFVVDGQ GRKMSKSVGN VVAPKDVTNK LGADILRLWV ASTDYTGEVA 650
    VSDEILKRSA DAYRRIRNTA RFFLANLNGF NPETDLVAPE EMVALDRWAV 700
    GRAMEAQEEI IKAYDGYNLH GVTQRLMQFC SVEMGSFYLD VIKDRQYTAK 750
    QGGHAQRSCQ TALFYIVEAL VRWMAPIMSF TADEIWNEMP ASQGNGEQRD 800
    KFVFTGEWFE GLFGLADDEV MNDEFWAEIQ QVRGAVNKLL ELARKDKVIG 850
    GSLQAEITLH ANETLAAKLN TLEDELRFVL LTSKAAVAIT DSMPEGAQKT 900
    DVEGLFVTVN ASEAAKCDRC WHHVADVGTI EGHEEVCGRC VTNISGEGEE 950
    RKFA 954
    Length:954
    Mass (Da):106,622
    Last modified:July 5, 2004 - v1
    Checksum:i59804D1020D1938F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378664 Genomic DNA. Translation: CAG19014.1.
    RefSeqiYP_128816.1. NC_006370.1.

    Genome annotation databases

    EnsemblBacteriaiCAG19014; CAG19014; PBPRA0591.
    GeneIDi3125510.
    KEGGippr:PBPRA0591.
    PATRICi22931874. VBIPhoPro109272_0669.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378664 Genomic DNA. Translation: CAG19014.1 .
    RefSeqi YP_128816.1. NC_006370.1.

    3D structure databases

    ProteinModelPortali Q6LUL1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 298386.PBPRA0591.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG19014 ; CAG19014 ; PBPRA0591 .
    GeneIDi 3125510.
    KEGGi ppr:PBPRA0591.
    PATRICi 22931874. VBIPhoPro109272_0669.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS9.

    Entry informationi

    Entry nameiSYI_PHOPR
    AccessioniPrimary (citable) accession number: Q6LUL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3