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Q6LUL1

- SYI_PHOPR

UniProt

Q6LUL1 - SYI_PHOPR

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721Aminoacyl-adenylateUniRule annotation
Binding sitei616 – 6161ATPUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation
Metal bindingi937 – 9371ZincUniRule annotation
Metal bindingi940 – 9401ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PBPRA0591
OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
Taxonomic identifieri74109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
ProteomesiUP000000593: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954Isoleucine--tRNA ligasePRO_0000098437Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRA0591.

Structurei

3D structure databases

ProteinModelPortaliQ6LUL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi613 – 6175"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LUL1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSDYKDTLNL PETGFPMRGN LAQREPVMLK RWDDEDLYGE IRKAKKGNKS
60 70 80 90 100
FILHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YIPGWDCHGL
110 120 130 140 150
PIELMVEKKW GKPGRKLTAA EFRQKCREYA AGQVEGQKES FIRLGVLGQW
160 170 180 190 200
DKPYRTMDFA TEANIIRSLG KVAENDHLLK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYQDKVSPSI DVKFKAVDEA GVVAKFNCAE GHEGQGDVSV VIWTTTPWTM
260 270 280 290 300
PANRAVAVRD DLEYVLVQVE ADAANDKAAY RLIVAAELAK DVMDRAGIEH
310 320 330 340 350
FHNLGFCKGA DLELMRFNHP FYSFDVPIVL GDHVTTESGT GCVHTAPGHG
360 370 380 390 400
QEDFVVGQKY GLEIANPVGS NGVYLPDTEL FAGQHVFKAN DVVVETLKEH
410 420 430 440 450
GALLHHHAYE HSYPHCWRHK TPIIFRATPQ WFISMDKAGL RAKALGEIKN
460 470 480 490 500
VQWLPEWGQS RIEGMVEGRP EWCISRQRTW GVPIALFVHK ETQELHPDTR
510 520 530 540 550
VLIEKVAQLV EQKGIQAWWD LNPAELMGEA DAANYEKVLD TLDVWFDSGV
560 570 580 590 600
THFSVVDSRE EYNGHSADLY LEGSDQHRGW FQSSLVSSVA MKGKAPYNQV
610 620 630 640 650
LTHGFVVDGQ GRKMSKSVGN VVAPKDVTNK LGADILRLWV ASTDYTGEVA
660 670 680 690 700
VSDEILKRSA DAYRRIRNTA RFFLANLNGF NPETDLVAPE EMVALDRWAV
710 720 730 740 750
GRAMEAQEEI IKAYDGYNLH GVTQRLMQFC SVEMGSFYLD VIKDRQYTAK
760 770 780 790 800
QGGHAQRSCQ TALFYIVEAL VRWMAPIMSF TADEIWNEMP ASQGNGEQRD
810 820 830 840 850
KFVFTGEWFE GLFGLADDEV MNDEFWAEIQ QVRGAVNKLL ELARKDKVIG
860 870 880 890 900
GSLQAEITLH ANETLAAKLN TLEDELRFVL LTSKAAVAIT DSMPEGAQKT
910 920 930 940 950
DVEGLFVTVN ASEAAKCDRC WHHVADVGTI EGHEEVCGRC VTNISGEGEE

RKFA
Length:954
Mass (Da):106,622
Last modified:July 5, 2004 - v1
Checksum:i59804D1020D1938F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378664 Genomic DNA. Translation: CAG19014.1.
RefSeqiYP_128816.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG19014; CAG19014; PBPRA0591.
GeneIDi3125510.
KEGGippr:PBPRA0591.
PATRICi22931874. VBIPhoPro109272_0669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378664 Genomic DNA. Translation: CAG19014.1 .
RefSeqi YP_128816.1. NC_006370.1.

3D structure databases

ProteinModelPortali Q6LUL1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 298386.PBPRA0591.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG19014 ; CAG19014 ; PBPRA0591 .
GeneIDi 3125510.
KEGGi ppr:PBPRA0591.
PATRICi 22931874. VBIPhoPro109272_0669.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS9.

Entry informationi

Entry nameiSYI_PHOPR
AccessioniPrimary (citable) accession number: Q6LUL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3