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Q6LUL1

- SYI_PHOPR

UniProt

Q6LUL1 - SYI_PHOPR

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PBPRA0591
Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721Aminoacyl-adenylate By similarity
Binding sitei616 – 6161ATP By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi937 – 9371Zinc By similarity
Metal bindingi940 – 9401Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PBPRA0591
OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
Taxonomic identifieri74109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
ProteomesiUP000000593: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098437Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRA0591.

Structurei

3D structure databases

ProteinModelPortaliQ6LUL1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi613 – 6175"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LUL1-1 [UniParc]FASTAAdd to Basket

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MSDYKDTLNL PETGFPMRGN LAQREPVMLK RWDDEDLYGE IRKAKKGNKS    50
FILHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YIPGWDCHGL 100
PIELMVEKKW GKPGRKLTAA EFRQKCREYA AGQVEGQKES FIRLGVLGQW 150
DKPYRTMDFA TEANIIRSLG KVAENDHLLK GFKPVHWCTD CGSALAEAEV 200
EYQDKVSPSI DVKFKAVDEA GVVAKFNCAE GHEGQGDVSV VIWTTTPWTM 250
PANRAVAVRD DLEYVLVQVE ADAANDKAAY RLIVAAELAK DVMDRAGIEH 300
FHNLGFCKGA DLELMRFNHP FYSFDVPIVL GDHVTTESGT GCVHTAPGHG 350
QEDFVVGQKY GLEIANPVGS NGVYLPDTEL FAGQHVFKAN DVVVETLKEH 400
GALLHHHAYE HSYPHCWRHK TPIIFRATPQ WFISMDKAGL RAKALGEIKN 450
VQWLPEWGQS RIEGMVEGRP EWCISRQRTW GVPIALFVHK ETQELHPDTR 500
VLIEKVAQLV EQKGIQAWWD LNPAELMGEA DAANYEKVLD TLDVWFDSGV 550
THFSVVDSRE EYNGHSADLY LEGSDQHRGW FQSSLVSSVA MKGKAPYNQV 600
LTHGFVVDGQ GRKMSKSVGN VVAPKDVTNK LGADILRLWV ASTDYTGEVA 650
VSDEILKRSA DAYRRIRNTA RFFLANLNGF NPETDLVAPE EMVALDRWAV 700
GRAMEAQEEI IKAYDGYNLH GVTQRLMQFC SVEMGSFYLD VIKDRQYTAK 750
QGGHAQRSCQ TALFYIVEAL VRWMAPIMSF TADEIWNEMP ASQGNGEQRD 800
KFVFTGEWFE GLFGLADDEV MNDEFWAEIQ QVRGAVNKLL ELARKDKVIG 850
GSLQAEITLH ANETLAAKLN TLEDELRFVL LTSKAAVAIT DSMPEGAQKT 900
DVEGLFVTVN ASEAAKCDRC WHHVADVGTI EGHEEVCGRC VTNISGEGEE 950
RKFA 954
Length:954
Mass (Da):106,622
Last modified:July 5, 2004 - v1
Checksum:i59804D1020D1938F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR378664 Genomic DNA. Translation: CAG19014.1.
RefSeqiYP_128816.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG19014; CAG19014; PBPRA0591.
GeneIDi3125510.
KEGGippr:PBPRA0591.
PATRICi22931874. VBIPhoPro109272_0669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR378664 Genomic DNA. Translation: CAG19014.1 .
RefSeqi YP_128816.1. NC_006370.1.

3D structure databases

ProteinModelPortali Q6LUL1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 298386.PBPRA0591.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG19014 ; CAG19014 ; PBPRA0591 .
GeneIDi 3125510.
KEGGi ppr:PBPRA0591.
PATRICi 22931874. VBIPhoPro109272_0669.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS9.

Entry informationi

Entry nameiSYI_PHOPR
AccessioniPrimary (citable) accession number: Q6LUL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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