Q6LU34 (CHEB1_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	PBPRA0778

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225466
Regions
	Domain	1 – 116	116	Response regulatory
	Domain	160 – 350	191	CheB-type methylesterase
Sites
	Active site	172	1	By similarity
	Active site	198	1	By similarity
	Active site	294	1	By similarity
Amino acid modifications
	Modified residue	50	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LU34 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5C6695A26E80CC35	FASTA	350	37,297
	10 20 30 40 50 60 MVVDDSAVVR QVVSSVLKSD SEIEVCGAVA DPLFAMTRMR MQWPDVIVLD IEMPRMDGIS 70 80 90 100 110 120 FLKKIMAERP TPVVICSTLT EKGADTTMQA ISAGAVEIIT KPKVGLKGFL HDSAKVLINA 130 140 150 160 170 180 VKAASRANLK PLQRAMASNL KVTPKLSADA VLAEGNTSRL KTTEQLVAIG TSTGGTQALE 190 200 210 220 230 240 LVLKALPRVS PGIVIVQHMP EKFTAAFAER LDSLCEISVK EAKHKMRVLP GQALIAPGGK 250 260 270 280 290 300 HMLLKRSGAQ YYVEVIDGPL VSRHRPSVDV LFRSVAQSAS GNALGIIMTG MGDDGVKGML 310 320 330 340 350 EMRRAGAVTL AQDEASCVVY GMPKEAVKCG AVERSLSLSE IPQAILDCSH

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378665 Genomic DNA. Translation: CAG19191.1.
3D structure databases
ProteinModelPortal	Q6LU34.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus PBPRA0778 in contig CR354531_GR.
KEGG	ppr:PBPRA0778.
NMPDR	fig|298386.1.peg.3003.
PATRIC	22932269. VBIPhoPro109272_0856.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q6LU34.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA0778-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_PHOPR

Accession

Primary (citable) accession number: Q6LU34

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program