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Protein

Glutamate 5-kinase

Gene

proB

Organism
Photobacterium profundum (strain SS9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20ATPUniRule annotation1
Binding sitei60SubstrateUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei159Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 180ATPUniRule annotation2
Nucleotide bindingi221 – 227ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:PBPRA0839
OrganismiPhotobacterium profundum (strain SS9)
Taxonomic identifieri298386 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000000593 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097041 – 378Glutamate 5-kinaseAdd BLAST378

Proteomic databases

PRIDEiQ6LTX3.

Interactioni

Protein-protein interaction databases

STRINGi298386.PBPRA0839.

Structurei

3D structure databases

ProteinModelPortaliQ6LTX3.
SMRiQ6LTX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 364PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OrthoDBiPOG091H00OF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6LTX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPPLSDKQT LPPKQTIVVK LGTSVLTGGT LKLDRAHMVE LVRQCAYLRR
60 70 80 90 100
YGHKVIIVTS GAIAAGREHL DYPELPKTMA SKQLLAAVGQ SRLIQEWESL
110 120 130 140 150
FGIYGLHVGQ MLLTRADLDD RERYLNARDM LIALLDNGII PVVNENDAVA
160 170 180 190 200
TTEIKVGDND NLSALVGILG GADKLLLLTD QPGLFTADPR NNPDAELIRE
210 220 230 240 250
VHTIDETLRK LAGGSAGGLG TGGMATKLQA ADVARRAGIE VIIAAGSRPD
260 270 280 290 300
VISDLASGES VGTRFLPLDT PLESRKRWIL AGPPPAGDIV IDAGAAKAVL
310 320 330 340 350
ERGSSLLSKG ISEVKGAFER GEVARIFDTN GVLLARGICR YSSRDMAMIV
360 370
GKHSQEIYKV LGYEYGPVAI HRDDLVVI
Length:378
Mass (Da):40,523
Last modified:July 5, 2004 - v1
Checksum:i81E7943C9C30EDB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378665 Genomic DNA. Translation: CAG19252.1.
RefSeqiWP_011217589.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG19252; CAG19252; PBPRA0839.
KEGGippr:PBPRA0839.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPROB_PHOPR
AccessioniPrimary (citable) accession number: Q6LTX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: June 7, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families