Reviewed, UniProtKB/Swiss-Prot Q6LTV9 (NAPA1_PHOPR)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase 1
    EC=1.7.99.4

Gene names

Name:	napA1
Ordered Locus Names:	PBPRA0853

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 828	798	Periplasmic nitrate reductase 1 HAMAP MF_01630		PRO_0000045993
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LTV9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 58C80D6AE7BC8A63	FASTA	828	93,366
	10 20 30 40 50 60 MKMTRRAFVK ANAAASAAAV AGITLPASAA NLIVSSDETK IKWDKAPCRF CGTGCSVLVG 70 80 90 100 110 120 TQNGRVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRLQ TPMLRMKNGE YNKEGDFAPV 130 140 150 160 170 180 SWDQAFDVMA EKFKKALKEK GPTSVGMFGS GQWTVMEGYA ASKMMKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVGGFM RTFGIDEPMG CYDDFEHADS FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK 250 260 270 280 290 300 VNVLSTYYHR SFELADNGMI FEPQTDLAIA NFIANYIIQN DAVNWDFVTK HTHFKRAETD 310 320 330 340 350 360 IGYGLRDDNP LQMKAKHPNS GALHPMDFEQ YKASVAEYTV EKASQMSGVS EEKLIEMAKQ 370 380 390 400 410 420 YADPKVKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS QPGNSPFSLT GQPSACGTAR 430 440 450 460 470 480 EVGTFSHRLP ADMVVANPKH RAIAEKIWKL PDGTIPAKPG YHAVQQDRML KDGKLNAYWV 490 500 510 520 530 540 MCNNNMQAGP NINEERLPGY RNPENFIVCS DPYPTVTAQA SDLILPTAMW VEKEGAYGNA 550 560 570 580 590 600 ERRTQAWYQQ VKSQGDSKSD LWQLMEFSKR FKIEEVWGED LLAQMPEYRG KTMYEVLFRN 610 620 630 640 650 660 GQVDKFPLSE AQELNDDAKA QGFYLQKGLF EEYAAFGRGH GHDLAPYDVY HQVRGLRWPV 670 680 690 700 710 720 VDGKETLWRF VEGSDPYVPE GEGFRFYGKP DGKANIIFAP FEPAPEEPDQ EYDMWLCTGR 730 740 750 760 770 780 VLEHWHTGTM TRRVPELYKA VPDALCFIHP DDAQKRGLRR GDEVLLESRR GEVRCRVETR 790 800 810 820 GRNRPPVGLV FVPFFDARVL VNKLILDATD PLSKQTDYKK CPIKITKV

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378665 Genomic DNA. Translation: CAG19266.1.
RefSeq	YP_129068.1.
3D structure databases
SMR	Q6LTV9. Positions 41-827.
ModBase	Search...
Genome annotation databases
GeneID	3123080.
KEGG	ppr:PBPRA0853.
NMPDR	fig|298386.1.peg.3083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG715033.
OMA	IISAPYE.
PhylomeDB	Q6LTV9.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA0853-MONOMER.
BRENDA	1.7.99.4. 263915.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA1_PHOPR

Accession

Primary (citable) accession number: Q6LTV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)