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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei125 – 1251Important for catalytic activityUniRule annotation
Sitei147 – 1471Important for catalytic activityUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:PBPRA0962
OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
Taxonomic identifieri74109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
ProteomesiUP000000593: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Fatty acid oxidation complex subunit alphaPRO_0000109302Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRA0962.

Structurei

3D structure databases

ProteinModelPortaliQ6LTK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 197190Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni313 – 7154033-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LTK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQHESANSQ PSAFSLTFGD NGVAWLKIDV PNERMNTLQS AFVDQVTDVL
60 70 80 90 100
AQLKDKKDIK GMVVYSGKPD NFIAGADIRM LAACQTADEA QQLAAKGQEL
110 120 130 140 150
FGQLEALPFH VVAAIHGPCL GGGLELALAC HSRVCSDDDK TRLGLPEVQL
160 170 180 190 200
GLLPGSGGTQ RLPRLIGVAN ALDMILTGKQ LRAKKAKNLG LVEEAVPLSI
210 220 230 240 250
LLDIAEKQAL KGKPKRKGSF QEWAMGGNAL GRSVVFDQAA KKTHEKTRGN
260 270 280 290 300
YPAADAILDV IKYGLQHGMK KGLDQEAKRF GELVMTSESA ALRSIFFATT
310 320 330 340 350
AMKKESGSDA LPATIKKVGV LGGGLMGGGI SHVTATKAGY PVRIKDISND
360 370 380 390 400
GIKNALVYNF KLLDKQRKRR IISKAELQNK MFSITGGTTF TGFTNVDVVI
410 420 430 440 450
EAVFEDINLK HQMVKDIEQQ TSDYTIFASN TSSLPIHQIA AAAERPENVV
460 470 480 490 500
GLHYFSPVEK MPLVEVIPHQ GTENGSATSE QTIATVVALA KKQGKTPIVV
510 520 530 540 550
ADKAGFYVNR ILAPYMNEAA AVLLSGEPIE HIDRSLLDFG FPVGPITLLD
560 570 580 590 600
EVGVDIGAKI SPILLAELGE RFKAPDVFDL LLSDDRKGRK SGKGFYLYNT
610 620 630 640 650
KKKEVDKSVY KLLSLEPEQK AAGQDIALRC TLMMLNEAAR CLDEGVIKSA
660 670 680 690 700
RDGDIGAIFG IGFPPFLGGP FRYMDTLGAK RVVEMLKDHT DKYGGRFTPC
710
DKLVAMANEE QSFYS
Length:715
Mass (Da):77,342
Last modified:July 5, 2004 - v1
Checksum:iBEDB6D9C220C07A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378666 Genomic DNA. Translation: CAG19373.1.
RefSeqiYP_129175.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG19373; CAG19373; PBPRA0962.
GeneIDi3125191.
KEGGippr:PBPRA0962.
PATRICi22932665. VBIPhoPro109272_1052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378666 Genomic DNA. Translation: CAG19373.1.
RefSeqiYP_129175.1. NC_006370.1.

3D structure databases

ProteinModelPortaliQ6LTK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298386.PBPRA0962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG19373; CAG19373; PBPRA0962.
GeneIDi3125191.
KEGGippr:PBPRA0962.
PATRICi22932665. VBIPhoPro109272_1052.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Enzyme and pathway databases

UniPathwayiUPA00659.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS9.

Entry informationi

Entry nameiFADJ_PHOPR
AccessioniPrimary (citable) accession number: Q6LTK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.