Reviewed, UniProtKB/Swiss-Prot Q6LT67 (SYC1_PHOPR)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase 1
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 1
      Short name=CysRS 1

Gene names

Name:	cysS1
Ordered Locus Names:	PBPRA1098

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Cysteinyl-tRNA synthetase 1 HAMAP MF_00041		PRO_0000159453
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LT67-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5853E76518677407	FASTA	464	52,294
	10 20 30 40 50 60 MLKIYNSLTK QKEEFKPIQP GKIGMYVCGV TIYDLCHIGH GRTFVSFDVV SRYLRYSGYD 70 80 90 100 110 120 LTFVRNITDI DDKIIKRAAE NGESCESLTE RLIGEMHKDF DALGMKRPDI EPRATEFIAE 130 140 150 160 170 180 IIILCERLIE RGFAYVASNG DVMFEVSKFE EYGRLSRQDL DQLQAGARVD IDMAKRSPLD 190 200 210 220 230 240 FVLWKMSKPG EPTWESPWGA GRPGWHIECS AMNSAILGDH FDIHGGGSDL QFPHHENEIA 250 260 270 280 290 300 QSCCATGSQY VNTWMHSGMV MVDREKMSKS LGNFFTIRDV LAHYDAESVR YFLMSGHYRS 310 320 330 340 350 360 QLNYSEDNLK QARSALERLY TSLRGLDLTA QAEGGEEFVT RFTASMNDDF NTPEAYSVLF 370 380 390 400 410 420 DMAREINRLK ADETIANNIV QASALGARLR ELADILGLLS QDPEAFLQGG AGQDDVAEIE 430 440 450 460 TLVQQRLDAR AAKDWAAADA ARDKLLAMKI ILEDGPQGTT WRRK

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378666 Genomic DNA. Translation: CAG19509.1.
RefSeq	YP_129311.1.
3D structure databases
SMR	Q6LT67. Positions 1-464.
ModBase	Search...
Genome annotation databases
GeneID	3124958.
KEGG	ppr:PBPRA1098.
NMPDR	fig|298386.1.peg.3337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	VLWKAAK.
PhylomeDB	Q6LT67.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA1098-MONOMER.
BRENDA	6.1.1.16. 263915.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_PHOPR

Accession

Primary (citable) accession number: Q6LT67

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)