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Reviewed, UniProtKB/Swiss-Prot Q6LSQ7 (NRFA_PHOPR)

Last modified June 16, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: PBPRA1258
OrganismPhotobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]
Taxonomic identifier74109 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

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Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 487460Cytochrome c-552 HAMAP MF_01182
PRO_0000268970

Sites

Metal binding1041Iron (heme 3 axial ligand) By similarity
Metal binding1361Iron (heme 1 axial ligand) By similarity
Metal binding1741Iron (heme 2 axial ligand) By similarity
Metal binding2231Iron (heme 3 axial ligand) By similarity
Metal binding2251Calcium By similarity
Metal binding2261Calcium; via carbonyl oxygen By similarity
Metal binding2711Calcium; via carbonyl oxygen By similarity
Metal binding2731Calcium By similarity
Metal binding2851Iron (heme 5 axial ligand) By similarity
Metal binding2961Iron (heme 4 axial ligand) By similarity
Metal binding3111Iron (heme 2 axial ligand) By similarity
Metal binding3281Iron (heme 5 axial ligand) By similarity
Metal binding4031Iron (heme 4 axial ligand) By similarity
Binding site1321Heme 1 (covalent) By similarity
Binding site1351Heme 1 (covalent) By similarity
Binding site1701Heme 2 (covalent) By similarity
Binding site1731Heme 2 (covalent) By similarity
Binding site2191Heme 3 (covalent) By similarity
Binding site2221Heme 3 (covalent) By similarity
Binding site2261Substrate By similarity
Binding site2741Substrate By similarity
Binding site2921Heme 4 (covalent) By similarity
Binding site2951Heme 4 (covalent) By similarity
Binding site3241Heme 5 (covalent) By similarity
Binding site3271Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q6LSQ7-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 14A8CF5628D882D7

FASTA48754,516
        10         20         30         40         50         60 
MSKKWTRNTA AMAAILSALC LSTNALATSE QAVTDENQTT TQQEGMRFEP RNEKFAEAHA 

        70         80         90        100        110        120 
EQYKSWKATQ ESEEIEDALE GDPNMVVLWA GYGFSKDYNK ARGHFYAITD VRETLRTGGP 

       130        140        150        160        170        180 
TDDKSGPMPM ACWSCKSPDV ARVIDDRGED GYFEGKWARL GAEINNAIGC SDCHNTGSEE 

       190        200        210        220        230        240 
FAKGGPALQM SRPYAERAME TIGKKFEDQS RFDQQAQVCG QCHVEYYFTG PKKSVKFPWD 

       250        260        270        280        290        300 
KGTSVDDMEV YYDEIGFKDW THGVSKAPML KAQHPGYETW REGIHGKNNV TCIDCHMPKV 

       310        320        330        340        350        360 
QNEDGTVFTD HKVGNPFDRF EDTCAQCHTQ TKAALKSVVA TRKASVLEMK LRAEKQIIAA 

       370        380        390        400        410        420 
HFEAKAAWDA GATEAEMEPI LMDIRHAQWR WDYAIASHGV HMHAPEVALR ILGTALDKAG 

       430        440        450        460        470        480 
DARAKVIRLL ATKGITEEIQ IPDISTKVKA QQALGMDMNK MNAEKDEFLK TIVPEWDKQA 


DAREAKY

« Hide

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References

[1]"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CR378667 Genomic DNA. Translation: CAG19669.1.
RefSeqYP_129471.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3124830.
GenomeReviewsGene locus PBPRA1258 in contig CR354531_GR.
KEGGppr:PBPRA1258.
NMPDRfig|298386.1.peg.3508.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ6LSQ7.
OMAQ6LSQ7. RDINSAC.

Enzyme and pathway databases

BioCycPPRO298386:PBPRA1258-MON.
BRENDA1.7.2.2. 263915.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_PHOPR
AccessionPrimary (citable) accession number: Q6LSQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 5, 2004
Last modified: June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents