Reviewed, UniProtKB/Swiss-Prot Q6LRD9 (TDH_PHOPR)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase
    EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	PBPRA1730

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_0000160848
Sites
	Metal binding	40	1	Zinc 1; catalytic By similarity
	Metal binding	65	1	Zinc 1; catalytic By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	150	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LRD9-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 9840A10643325FD1	FASTA	343	37,435
	10 20 30 40 50 60 MKIKALSKLK PEEGIWMNEV DKPEMGHNDL LIRIKKTAIC GTDVHIYNWD EWSQKTIPVP 70 80 90 100 110 120 MVVGHEYVGE VVGIGQEVRG FTIGDRVSGE GHITCGHCRN CRGGRTHLCR NTTGVGVNRD 130 140 150 160 170 180 GAFAEFLVIP AFNAFKIPAG ISDDLASIFD PFGNAVHTAL SFDLVGEDVL ITGAGPIGIM 190 200 210 220 230 240 AAAVAKHVGA RHVVITDVNE YRLDLARKMG VTRAVNVANE KLEDVMSELG MTEGFDVGLE 250 260 270 280 290 300 MSGVPSAFNG MLASMNHGGK VALLGIPPSD MAVDWTQVIF KGLVIKGIYG REMFETWYKM 310 320 330 340 ASLIQSGLDL TPIITHHYKI DDFQKGFDMM RSGMSGKVIL DWE

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378668 Genomic DNA. Translation: CAG20137.1.
RefSeq	YP_129939.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3125455.
GenomeReviews	Gene locus PBPRA1730 in contig CR354531_GR.
KEGG	ppr:PBPRA1730.
NMPDR	fig|298386.1.peg.3992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LRD9.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA1730-MON.
BRENDA	1.1.1.103. 263915.
Family and domain databases
HAMAP	MF_00627. [Tree]
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR004627. L-Threonine_3-DHase. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_PHOPR

Accession

Primary (citable) accession number: Q6LRD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	September 22, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)