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Q6LRA0 (RLMKL_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

  1. 23S rRNA m2G2445 methyltransferase
    EC=2.1.1.173
    Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase RlmL
  2. 23S rRNA m7G2069 methyltransferase
    EC=2.1.1.264
    Alternative name(s):
    rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene names
Name:rlmL
Ordered Locus Names:PBPRA1769
OrganismPhotobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]
Taxonomic identifier74109 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858

Catalytic activity

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01858.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmKL family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(2445)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 712712Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858
PRO_0000366780

Regions

Domain43 – 154112THUMP

Sequences

Sequence LengthMass (Da)Tools
Q6LRA0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B36C85C6AFE572C6

FASTA71280,413
        10         20         30         40         50         60 
MNQYLAITSR GLENLLAEEL EQLGAQSIQV VHAGVRFKAD QSTAYRCCLW TRISSRIIQV 

        70         80         90        100        110        120 
LSEFTVRDDM DLYLGAAAIN WTEYFSNATR IVVDFNGTNR EIRNSQYGAM KVKDAIVDRF 

       130        140        150        160        170        180 
TKADLRRPNI DREQPDLRIH MRLSGEKGVL GLDMAGSGLH QRGYRTEAGR APLRETHAAA 

       190        200        210        220        230        240 
LVLKSGWTPG QPLLDPMCGS GTLLIEAAMM AADIAPGLKR KRWGFESIKD FDKDAWLEIH 

       250        260        270        280        290        300 
AEASVKARRG PAKVQTKFFG FELEHRILAI ARDNAGRAGV KELINFQQGD ATELKAPEGF 

       310        320        330        340        350        360 
DAGIVICNPP YGERLGTTPE LISLYTELGN RLKLAFAGSS ASIYSGSNEL LSCLRMRADK 

       370        380        390        400        410        420 
QFKLPNGALD CVLKTYLITA GSVKKEEGEA EGHVEQENVA PDFANRLKKN ITKLNKWAKK 

       430        440        450        460        470        480 
EGIDCYRIYD ADLPNYNAAI DKYNDYLIIQ EYAAPKTVPE EIARRRIMDV LRATIEVTGV 

       490        500        510        520        530        540 
QTDKVILKVR ERQKGKNQYQ KLSNAERHII VNEYGVDLKV NLYDYLDTGL FLDHRITRKM 

       550        560        570        580        590        600 
LGDMAKGKDF LNLFAYTGSA SVHAACGGAK STTTIDMSNT YLGWAQENME LNNQVGDQHE 

       610        620        630        640        650        660 
FIQADCLQWL QEVDDTFDLI FIDPPTFSNS KRMKQTFDIQ RDHIMLMENL KRMLRTDGKI 

       670        680        690        700        710 
VFSNNKRQFK MDLEKLNELG LDAKNISDKT LPMDFAKNKH IHNSWIITHK ED 

« Hide

References

[1]"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SS9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR378668 Genomic DNA. Translation: CAG20176.1.
RefSeqYP_129978.1. NC_006370.1.

3D structure databases

ProteinModelPortalQ6LRA0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING298386.PBPRA1769.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG20176; CAG20176; PBPRA1769.
GeneID3123889.
KEGGppr:PBPRA1769.
PATRIC22934429. VBIPhoPro109272_1925.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0116.
HOGENOMHOG000218371.
KOK12297.
OMANKRGFKM.
OrthoDBEOG6HJ238.

Family and domain databases

Gene3D3.40.50.150. 3 hits.
HAMAPMF_01858. 23SrRNA_methyltr_KL.
InterProIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases-like.
IPR004114. THUMP.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 3 hits.
PROSITEPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMKL_PHOPR
AccessionPrimary (citable) accession number: Q6LRA0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families