NAD-dependent malic enzyme 1 - Photobacterium profundum

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Reviewed, UniProtKB/Swiss-Prot Q6LQM6 (MAO11_PHOPR)

Last modified February 9, 2010. Version 41. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme 1
      Short name=NAD-ME 1
    EC=1.1.1.38

Gene names

Name:	sfcA1
Ordered Locus Names:	PBPRA1997

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = pyruvate + CO₂ + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

562

NAD-dependent malic enzyme 1 HAMAP MF_01619

PRO_0000160221

Sites

Active site

1

Proton donor By similarity

Active site

1

Proton acceptor By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Site

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6LQM6-1 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: 07347A2F21BAF062
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562

62,558

        10         20         30         40         50         60 
MNNDKRPLYI PYAGPALLET PLLNKGSAFS IEERMFFNLE GLLPEAIESI EEQTERAYQQ 

        70         80         90        100        110        120 
YQKFDKDMDK HIYLRNIQDT NETLFYRLVE NHITEMMPII YTPTVGAACE DFSNIYRRGR 

       130        140        150        160        170        180 
GLFISYPNRD RIEDMLNNAS RQNVKVIVVT DGERILGLGD QGIGGMGIPI GKLALYTACG 

       190        200        210        220        230        240 
GISPAYTLPV VLDVGTNNPQ RLSDPMYMGW RHTRISGQEY DNFVDEFIQA VKRRWPDALI 

       250        260        270        280        290        300 
QFEDFAQKNA MPLLERYKNK ICCFNDDIQG TAAVTVGSLL AACKAAKSKL SEQRISFLGA 

       310        320        330        340        350        360 
GSAGCGIAEA IIAQMVSEGI SEAQARSQVF MVDRWGLLID DMPNLLNFQQ ALVQKRDTVN 

       370        380        390        400        410        420 
EWDLADNNVS LLDVMRNAKP TVLIGVSGVP GLFSEDVIRE MHAHCKRPIV FPLSNPTSRV 

       430        440        450        460        470        480 
EATPADIINW TDGNALVATG SPFEPVVHNG KTYPIAQCNN SYIFPGIGLG ILAVNARRVT 

       490        500        510        520        530        540 
DEMLMASSRA LAECSPLAIN GHGALLPPLE DIQKVSRKIA FAVAKKAIEQ GKAPKNSDER 

       550        560 
ILDKIDANFW QSDYRNYKRT SF

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378669 Genomic DNA. Translation: CAG20400.1. Different initiation.
RefSeq	YP_130202.2.
3D structure databases
SMR	Q6LQM6. Positions 13-562.
ModBase	Search...
Genome annotation databases
GeneID	3122794.
KEGG	ppr:PBPRA1997.
NMPDR	fig\|298386.1.peg.4263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289821.
PhylomeDB	Q6LQM6.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA1997-MONOMER.
BRENDA	1.1.1.38. 263915.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO11_PHOPR

Accession

Primary (citable) accession number: Q6LQM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents