Reviewed, UniProtKB/Swiss-Prot Q6LQM6 (MAO11_PHOPR)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme 1
      Short name=NAD-ME 1
    EC=1.1.1.38

Gene names

Name:	sfcA1
Ordered Locus Names:	PBPRA1997

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme 1 HAMAP MF_01619		PRO_0000160221
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LQM6-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 07347A2F21BAF062	FASTA	562	62,558
	10 20 30 40 50 60 MNNDKRPLYI PYAGPALLET PLLNKGSAFS IEERMFFNLE GLLPEAIESI EEQTERAYQQ 70 80 90 100 110 120 YQKFDKDMDK HIYLRNIQDT NETLFYRLVE NHITEMMPII YTPTVGAACE DFSNIYRRGR 130 140 150 160 170 180 GLFISYPNRD RIEDMLNNAS RQNVKVIVVT DGERILGLGD QGIGGMGIPI GKLALYTACG 190 200 210 220 230 240 GISPAYTLPV VLDVGTNNPQ RLSDPMYMGW RHTRISGQEY DNFVDEFIQA VKRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKNK ICCFNDDIQG TAAVTVGSLL AACKAAKSKL SEQRISFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SEAQARSQVF MVDRWGLLID DMPNLLNFQQ ALVQKRDTVN 370 380 390 400 410 420 EWDLADNNVS LLDVMRNAKP TVLIGVSGVP GLFSEDVIRE MHAHCKRPIV FPLSNPTSRV 430 440 450 460 470 480 EATPADIINW TDGNALVATG SPFEPVVHNG KTYPIAQCNN SYIFPGIGLG ILAVNARRVT 490 500 510 520 530 540 DEMLMASSRA LAECSPLAIN GHGALLPPLE DIQKVSRKIA FAVAKKAIEQ GKAPKNSDER 550 560 ILDKIDANFW QSDYRNYKRT SF

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378669 Genomic DNA. Translation: CAG20400.1. Different initiation.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus PBPRA1997 in contig CR354531_GR.
KEGG	ppr:PBPRA1997.
NMPDR	fig|298386.1.peg.4263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LQM6.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA1997-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO11_PHOPR

Accession

Primary (citable) accession number: Q6LQM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)