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Q6LQJ3 (NAPA2_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase 2

EC=1.7.99.4
Gene names
Name:napA2
Ordered Locus Names:PBPRA2030
OrganismPhotobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]
Taxonomic identifier74109 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence caution

The sequence CAG20433.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 830799Periplasmic nitrate reductase 2 HAMAP-Rule MF_01630
PRO_0000045994

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6LQJ3 [UniParc].

Last modified February 7, 2006. Version 2.
Checksum: 80887A70897D1EDD

FASTA83092,868
        10         20         30         40         50         60 
MKVSRRKFIK AQAVASAAAA AGISIPISAS NVVTDSSLTK ITWEKAPCRF CGTGCSVNVG 

        70         80         90        100        110        120 
TKEGKVVATH GDIKSPVNRG LNCVKGYFLS KIMYGKDRLT QPLLRMTDGK FDKNGEFSPI 

       130        140        150        160        170        180 
SWDQAFDIMA EKWKKTLKEK GPEAIGMFGS GQWTIYDGYA ASKLMKAGFR SNNLEPNARH 

       190        200        210        220        230        240 
CMASAVVGFI RTFGIDEPMG CYDDIEAADA FVLWGSNMVE MHPILWTRVT DRRLSVPGVK 

       250        260        270        280        290        300 
VSVLSTFEHR SFELADIPII FTPQSDLAIL NFIANYIITN DKVNKDFTSK HVNFRRGTTD 

       310        320        330        340        350        360 
IGYGLRPEHP LQQAAKNPDS GDSTPMTFEE FKAFVSEYTV EKASEISGVK PDKLIELAEL 

       370        380        390        400        410        420 
YANPETKVVS FWTMGFNQHT RGVWCNNLVY NIHLLTGKIS TPGNSPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFAHRLP ADLVVTNPEH RKIAEKIWKL PDGTIPGKVG AHAVLQSRML KDGKINAYWI 

       490        500        510        520        530        540 
QVNNNLQAGA NINEETLPGY RNPDNFIVVS EAYPTVTSQA ADLVLPTAMW VEKEGGFGNA 

       550        560        570        580        590        600 
ERRTQLWHQM VEAPGESKPD LWQLMEFSKR FKTDEVWPKE LLDSNPDYKG KTLFDVLFIN 

       610        620        630        640        650        660 
GNVNKYPLDD LAEGTLNDES NYYGFYVHKG LFEEYAEFGR GHGHDLAPFD MYHETRGLRW 

       670        680        690        700        710        720 
PVVDGKETLW RYREGSDPYV KEGAGFEFYG KPDGKAIIFA LPYEPAAESP DEEYDMWLST 

       730        740        750        760        770        780 
GRVIEHWHSG SMTQRVPELY KAYPDAQVFM HPDDAKSRGL RRGDAVKVMS RRGEIITRVE 

       790        800        810        820        830 
TRGRNKPPIG LVFVPWFDAS QLINKVTLDA TDPLSKQTDF KKCAVKIVKA 

« Hide

References

[1]"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SS9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR378669 Genomic DNA. Translation: CAG20433.1. Different initiation.

3D structure databases

ProteinModelPortalQ6LQJ3.
SMRQ6LQJ3. Positions 41-829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING298386.PBPRA2030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG20433; CAG20433; PBPRA2030.
PATRIC22934984. VBIPhoPro109272_2201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA2_PHOPR
AccessionPrimary (citable) accession number: Q6LQJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: February 19, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families