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Q6LQJ3

- NAPA2_PHOPR

UniProt

Q6LQJ3 - NAPA2_PHOPR

Protein

Periplasmic nitrate reductase 2

Gene

napA2

Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductase 2UniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napA2UniRule annotation
    Ordered Locus Names:PBPRA2030
    OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
    Taxonomic identifieri74109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
    ProteomesiUP000000593: Chromosome 1

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 830799Periplasmic nitrate reductase 2PRO_0000045994Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi298386.PBPRA2030.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LQJ3.
    SMRiQ6LQJ3. Positions 41-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6LQJ3-1 [UniParc]FASTAAdd to Basket

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    MKVSRRKFIK AQAVASAAAA AGISIPISAS NVVTDSSLTK ITWEKAPCRF    50
    CGTGCSVNVG TKEGKVVATH GDIKSPVNRG LNCVKGYFLS KIMYGKDRLT 100
    QPLLRMTDGK FDKNGEFSPI SWDQAFDIMA EKWKKTLKEK GPEAIGMFGS 150
    GQWTIYDGYA ASKLMKAGFR SNNLEPNARH CMASAVVGFI RTFGIDEPMG 200
    CYDDIEAADA FVLWGSNMVE MHPILWTRVT DRRLSVPGVK VSVLSTFEHR 250
    SFELADIPII FTPQSDLAIL NFIANYIITN DKVNKDFTSK HVNFRRGTTD 300
    IGYGLRPEHP LQQAAKNPDS GDSTPMTFEE FKAFVSEYTV EKASEISGVK 350
    PDKLIELAEL YANPETKVVS FWTMGFNQHT RGVWCNNLVY NIHLLTGKIS 400
    TPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADLVVTNPEH RKIAEKIWKL 450
    PDGTIPGKVG AHAVLQSRML KDGKINAYWI QVNNNLQAGA NINEETLPGY 500
    RNPDNFIVVS EAYPTVTSQA ADLVLPTAMW VEKEGGFGNA ERRTQLWHQM 550
    VEAPGESKPD LWQLMEFSKR FKTDEVWPKE LLDSNPDYKG KTLFDVLFIN 600
    GNVNKYPLDD LAEGTLNDES NYYGFYVHKG LFEEYAEFGR GHGHDLAPFD 650
    MYHETRGLRW PVVDGKETLW RYREGSDPYV KEGAGFEFYG KPDGKAIIFA 700
    LPYEPAAESP DEEYDMWLST GRVIEHWHSG SMTQRVPELY KAYPDAQVFM 750
    HPDDAKSRGL RRGDAVKVMS RRGEIITRVE TRGRNKPPIG LVFVPWFDAS 800
    QLINKVTLDA TDPLSKQTDF KKCAVKIVKA 830
    Length:830
    Mass (Da):92,868
    Last modified:February 7, 2006 - v2
    Checksum:i80887A70897D1EDD
    GO

    Sequence cautioni

    The sequence CAG20433.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378669 Genomic DNA. Translation: CAG20433.1. Different initiation.

    Genome annotation databases

    EnsemblBacteriaiCAG20433; CAG20433; PBPRA2030.
    PATRICi22934984. VBIPhoPro109272_2201.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378669 Genomic DNA. Translation: CAG20433.1 . Different initiation.

    3D structure databases

    ProteinModelPortali Q6LQJ3.
    SMRi Q6LQJ3. Positions 41-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 298386.PBPRA2030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG20433 ; CAG20433 ; PBPRA2030 .
    PATRICi 22934984. VBIPhoPro109272_2201.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    OrthoDBi EOG6CVV7G.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS9.

    Entry informationi

    Entry nameiNAPA2_PHOPR
    AccessioniPrimary (citable) accession number: Q6LQJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3