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Protein

Periplasmic nitrate reductase 2

Gene

napA2

Organism
Photobacterium profundum (strain SS9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei484Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei533Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei560Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei796Substrate; via amide nitrogenUniRule annotation1
Binding sitei804Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei821Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase 2UniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napA2UniRule annotation
Ordered Locus Names:PBPRA2030
OrganismiPhotobacterium profundum (strain SS9)
Taxonomic identifieri298386 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000000593 Componenti: Chromosome 1

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_000004599432 – 830Periplasmic nitrate reductase 2UniRule annotationAdd BLAST799

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiQ6LQJ3

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRA2030

Structurei

3D structure databases

ProteinModelPortaliQ6LQJ3
SMRiQ6LQJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni510 – 511Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni720 – 729Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6LQJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSRRKFIK AQAVASAAAA AGISIPISAS NVVTDSSLTK ITWEKAPCRF
60 70 80 90 100
CGTGCSVNVG TKEGKVVATH GDIKSPVNRG LNCVKGYFLS KIMYGKDRLT
110 120 130 140 150
QPLLRMTDGK FDKNGEFSPI SWDQAFDIMA EKWKKTLKEK GPEAIGMFGS
160 170 180 190 200
GQWTIYDGYA ASKLMKAGFR SNNLEPNARH CMASAVVGFI RTFGIDEPMG
210 220 230 240 250
CYDDIEAADA FVLWGSNMVE MHPILWTRVT DRRLSVPGVK VSVLSTFEHR
260 270 280 290 300
SFELADIPII FTPQSDLAIL NFIANYIITN DKVNKDFTSK HVNFRRGTTD
310 320 330 340 350
IGYGLRPEHP LQQAAKNPDS GDSTPMTFEE FKAFVSEYTV EKASEISGVK
360 370 380 390 400
PDKLIELAEL YANPETKVVS FWTMGFNQHT RGVWCNNLVY NIHLLTGKIS
410 420 430 440 450
TPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADLVVTNPEH RKIAEKIWKL
460 470 480 490 500
PDGTIPGKVG AHAVLQSRML KDGKINAYWI QVNNNLQAGA NINEETLPGY
510 520 530 540 550
RNPDNFIVVS EAYPTVTSQA ADLVLPTAMW VEKEGGFGNA ERRTQLWHQM
560 570 580 590 600
VEAPGESKPD LWQLMEFSKR FKTDEVWPKE LLDSNPDYKG KTLFDVLFIN
610 620 630 640 650
GNVNKYPLDD LAEGTLNDES NYYGFYVHKG LFEEYAEFGR GHGHDLAPFD
660 670 680 690 700
MYHETRGLRW PVVDGKETLW RYREGSDPYV KEGAGFEFYG KPDGKAIIFA
710 720 730 740 750
LPYEPAAESP DEEYDMWLST GRVIEHWHSG SMTQRVPELY KAYPDAQVFM
760 770 780 790 800
HPDDAKSRGL RRGDAVKVMS RRGEIITRVE TRGRNKPPIG LVFVPWFDAS
810 820 830
QLINKVTLDA TDPLSKQTDF KKCAVKIVKA
Length:830
Mass (Da):92,868
Last modified:February 7, 2006 - v2
Checksum:i80887A70897D1EDD
GO

Sequence cautioni

The sequence CAG20433 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378669 Genomic DNA Translation: CAG20433.1 Different initiation.
RefSeqiWP_041394329.1, NC_006370.1

Genome annotation databases

EnsemblBacteriaiCAG20433; CAG20433; PBPRA2030
KEGGippr:PBPRA2030

Similar proteinsi

Entry informationi

Entry nameiNAPA2_PHOPR
AccessioniPrimary (citable) accession number: Q6LQJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 23, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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