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Q6LN87

- LIPA_PHOPR

UniProt

Q6LN87 - LIPA_PHOPR

Protein

Lipoyl synthase

Gene

lipA

Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi95 – 951Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi99 – 991Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:PBPRA2896
    OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
    Taxonomic identifieri74109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
    ProteomesiUP000000593: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Lipoyl synthasePRO_1000012253Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi298386.PBPRA2896.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LN87.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiPNIAECF.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6LN87-1 [UniParc]FASTAAdd to Basket

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    MSKPIKIEQG IKYRDADKMA LIPVRNVAEE APKEVLRKPA WMKIKLPSDS    50
    KRIQEIKSAL RKNKLHSVCE EASCPNLAEC FNHGTATFMI LGAICTRRCP 100
    FCDVAHGRPL PPNAEEPSHL AQTIADMKLK YVVITSVDRD DLRDGGAQHF 150
    VDCIREIREK SPEIHIETLV PDFRGRMDRA LDILQGTPPN VFNHNLETAP 200
    RLYRKARPGA NYQWSLDLLK NFKEIHPEVP TKSGVMMGLG ETKEEIIQVL 250
    KDLRAHGVTM LTLGQYLAPS RHHLPVERYV PPAEFDELKE IALELGFTHA 300
    ACGPFVRSSY HADLQAQGLE IK 322
    Length:322
    Mass (Da):36,335
    Last modified:July 5, 2004 - v1
    Checksum:i31FD89F6825DF199
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378672 Genomic DNA. Translation: CAG21239.1.
    RefSeqiYP_131041.1. NC_006370.1.

    Genome annotation databases

    EnsemblBacteriaiCAG21239; CAG21239; PBPRA2896.
    GeneIDi3124044.
    KEGGippr:PBPRA2896.
    PATRICi22936824. VBIPhoPro109272_3066.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR378672 Genomic DNA. Translation: CAG21239.1 .
    RefSeqi YP_131041.1. NC_006370.1.

    3D structure databases

    ProteinModelPortali Q6LN87.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 298386.PBPRA2896.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG21239 ; CAG21239 ; PBPRA2896 .
    GeneIDi 3124044.
    KEGGi ppr:PBPRA2896.
    PATRICi 22936824. VBIPhoPro109272_3066.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi PNIAECF.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS9.

    Entry informationi

    Entry nameiLIPA_PHOPR
    AccessioniPrimary (citable) accession number: Q6LN87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3