Q6LN10 (QUEF_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	PBPRA2982

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817		PRO_0000163042

Sequences

	Sequence		Length	Mass (Da)
	Q6LN10 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 791238F4D8635610	FASTA	281	32,273
	10 20 30 40 50 60 MSKYKDAKEL AGLTLGQKTD YIDQYDASLL QPVPRSLNRS DLNLGESLPF TGYDIWTLYE 70 80 90 100 110 120 LSWLNSKGLP QVAIGEVRLP ASSPNLIESK SFKLYLNSFN QTRFDSWQQI ADTLQKDLTA 130 140 150 160 170 180 CAGQEVDVTI QPLEDFTDQT VINFAGNCID EQDIEINSYD FDAQYLKDAA EGEIVTEDLH 190 200 210 220 230 240 SHLLKSNCLI TNQPDWGSVK ISYKGKQINR EKLLRYLVSF RNHNEFHEQC VERIFTDIMK 250 260 270 280 FCQPELLTVY ARYTRRGGLD INPYRTNQGK TPSDNFRLAR Q

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378672 Genomic DNA. Translation: CAG21316.1.
RefSeq	YP_131118.1. NC_006370.1.
3D structure databases
ProteinModelPortal	Q6LN10.
SMR	Q6LN10. Positions 21-281.
ModBase	Search...
Protein-protein interaction databases
STRING	298386.PBPRA2982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG21316; CAG21316; PBPRA2982.
GeneID	3124050.
KEGG	ppr:PBPRA2982.
PATRIC	22937004. VBIPhoPro109272_3147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000273755.
KO	K06879.
OMA	ERIFCDL.
ProtClustDB	PRK11792.
Enzyme and pathway databases
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00817. QueF_type2.
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_PHOPR

Accession

Primary (citable) accession number: Q6LN10

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program