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Q6LM67

- CYSG_PHOPR

UniProt

Q6LM67 - CYSG_PHOPR

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Protein

Siroheme synthase

Gene
cysG, PBPRA3312
Organism
Photobacterium profundum (Photobacterium sp. (strain SS9))
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei246 – 2461Proton acceptor By similarity
Active sitei268 – 2681Proton donor By similarity
Binding sitei304 – 3041S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei381 – 3811S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei410 – 4101S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPRO298386-WGS:GSSB-3305-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:cysG
Ordered Locus Names:PBPRA3312
OrganismiPhotobacterium profundum (Photobacterium sp. (strain SS9))
Taxonomic identifieri74109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
ProteomesiUP000000593: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Siroheme synthaseUniRule annotationPRO_0000330529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi298386.PBPRA3312.

Structurei

3D structure databases

ProteinModelPortaliQ6LM67.
SMRiQ6LM67. Positions 1-455.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarityAdd
BLAST
Regioni214 – 469256Uroporphyrinogen-III C-methyltransferase By similarityAdd
BLAST
Regioni299 – 3013S-adenosyl-L-methionine binding By similarity
Regioni329 – 3302S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiEHATAYT.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LM67-1 [UniParc]FASTAAdd to Basket

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MDYLPIFTDL KRRPCLVVGG GDVAWRKARM LLKAGADVRV IAPVLNQAFQ    50
QAIEQQQVSH VADEFESSHL DGIFLAIAAT DRKAVNALVY QSANQRQVLV 100
NVVDDTQRCS FIIPSIIDRS PIIVAVSSSG KAPVLARLIR EKLEALLPQH 150
LGRMATIAGN FRARLAKTVT SFSARRQFWE QVFDGRFSDL VACGQEQDAK 200
QELINLTQST SPQGQVALIG SGPGDAGLLT LRALQLMQQA DVVLYDYLVS 250
DEVMDLVRRD AELVCVGKKA GFHSVPQEET NQLIVKYAQQ GKRVVRLKGG 300
DPFVFGRGGE ELEVLFDANI LFQVVPGITA AAGATAYAGI PLTHRDYAQT 350
AMFVTGHLKA ESDQMDWSTL ARGKQTLVIY MGLMKSGHIQ QQLLINGRAG 400
DTPIAIIERG TQKKQKVIKG QLSELEQLAK NAESPSLIVI GEVVNLSEKL 450
HWFGKQEQSL QQSAVVKLA 469
Length:469
Mass (Da):51,363
Last modified:July 5, 2004 - v1
Checksum:i79177BD9A4116649
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR378673 Genomic DNA. Translation: CAG21610.1.
RefSeqiYP_131412.1. NC_006370.1.

Genome annotation databases

EnsemblBacteriaiCAG21610; CAG21610; PBPRA3312.
GeneIDi3125518.
KEGGippr:PBPRA3312.
PATRICi22937691. VBIPhoPro109272_3454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR378673 Genomic DNA. Translation: CAG21610.1 .
RefSeqi YP_131412.1. NC_006370.1.

3D structure databases

ProteinModelPortali Q6LM67.
SMRi Q6LM67. Positions 1-455.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 298386.PBPRA3312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG21610 ; CAG21610 ; PBPRA3312 .
GeneIDi 3125518.
KEGGi ppr:PBPRA3312.
PATRICi 22937691. VBIPhoPro109272_3454.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi EHATAYT.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci PPRO298386-WGS:GSSB-3305-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS9.

Entry informationi

Entry nameiCYSG_PHOPR
AccessioniPrimary (citable) accession number: Q6LM67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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