Reviewed, UniProtKB/Swiss-Prot Q6LM59 (CYSI_PHOPR)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	PBPRA3320

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199904
Sites
	Metal binding	426	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	432	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	472	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	476	1	Iron (siroheme axial ligand) By similarity
	Metal binding	476	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LM59-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E103B8C14064EBEE	FASTA	563	62,719
	10 20 30 40 50 60 MTEQKLSDNE RLKRESNFLR GTIADDLQDR ITGGFTADNF QLIRFHGMYQ QDDRDIRAER 70 80 90 100 110 120 AKQKLEPLHN VMLRARMPGG IITPTQWLAI DKFAEESSLY GSIRLTTRQT FQFHGVLKPN 130 140 150 160 170 180 IKLMHQTLNS IGIDSIATAG DVNRNVLCTT NPIESELHQE AYEWAKKISE HLLPKTRAYA 190 200 210 220 230 240 EIWLDGEKVE GGDEEPILGS NYLPRKFKTT VVIPPQNDVD VHANDLNFIA IADNGKLVGF 250 260 270 280 290 300 NVLVGGGLAM THGDKTTYPR RADDFGFIPL VNTLEVAAAV VTTQRDWGNR SNRKNAKTKY 310 320 330 340 350 360 TLDRVGVDVF KAEVEKRAGI TFEASRPYEF TDRGDRIGWV EGIDGKHHLA LFIENGRLLD 370 380 390 400 410 420 TPGKPLKTGV AEIAKIHKGD FRMTANQNLI VAGVPAADKN KIEKIARDHG LIDDGVSEQR 430 440 450 460 470 480 KNSMACVAFP TCPLAMAEAE RFLPDFVTDV EGVLAKHGLA EDDNIILRVT GCANGCGRAM 490 500 510 520 530 540 LAEIGLVGKA PGRYNVHLGG NRNGTRVPKM YRENITVPQI MDEIDQLVGR WANEREDGED 550 560 FGDFTVRTGI IEPVLVSKRD FYA

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378673 Genomic DNA. Translation: CAG21618.1.
RefSeq	YP_131420.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOP based on UniProtKB P17846.
ModBase	Search...
Genome annotation databases
GeneID	3122708.
GenomeReviews	Gene locus PBPRA3320 in contig CR354531_GR.
KEGG	ppr:PBPRA3320.
NMPDR	fig|298386.1.peg.5527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LM59.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA3320-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_PHOPR

Accession

Primary (citable) accession number: Q6LM59

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)