Flavohemoprotein - Photobacterium profundum

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Reviewed, UniProtKB/Swiss-Prot Q6LM37 (HMP_PHOPR)

Last modified November 3, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Flavohemoprotein
Alternative name(s):
    Hemoglobin-like protein
    Flavohemoglobin
    Nitric oxide dioxygenase
      Short name=NO oxygenase
      Short name=NOD
    EC=1.14.12.17

Gene names

Name:	hmp
Ordered Locus Names:	PBPRA3343

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

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Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O₂ and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress By similarity.
Catalytic activity	2 NO + 2 O₂ + NAD(P)H = 2 NO₃^- + NAD(P)⁺. HAMAP MF_01252
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 1 FAD per subunit By similarity.
Domain	Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H. HAMAP MF_01252
Sequence similarities	Belongs to the globin family. Two-domain flavohemoproteins subfamily. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

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Ontologies

Keywords
Biological process	Detoxification Oxygen transport Transport
Ligand	FAD Flavoprotein Heme Iron Metal-binding NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen transport Inferred from electronic annotation. Source: HAMAP response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitric oxide dioxygenase activity Inferred from electronic annotation. Source: HAMAP oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 394

394

Flavohemoprotein HAMAP MF_01252

PRO_0000052438

Regions

Domain

150 – 255

106

FAD-binding FR-type

Nucleotide binding

204 – 207

FAD By similarity

Nucleotide binding

268 – 273

NADP By similarity

Nucleotide binding

387 – 390

FAD By similarity

Region

1 – 136

136

Globin HAMAP MF_01252

Region

147 – 394

248

Reductase HAMAP MF_01252

Region

259 – 394

136

NAD or NADP-binding HAMAP MF_01252

Sites

Active site

Charge relay system By similarity

Active site

135

Charge relay system By similarity

Metal binding

Iron (heme proximal ligand) By similarity

Binding site

188

FAD By similarity

Site

Involved in heme-bound ligand stabilization and O-O bond activation By similarity

Site

Influences the redox potential of the prosthetic heme and FAD groups By similarity

Site

386

Influences the redox potential of the prosthetic heme and FAD groups By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q6LM37-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: F7E15D173D44D18F
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FASTA

394

43,998

        10         20         30         40         50         60 
MISQQTIDIV KATAPVVAET GPKLTAHFYE RMFNHNPELK DIFNMSNQRN GDQREALFNA 

        70         80         90        100        110        120 
ICAYANNIDN LAALLPAVEK IAHKHTSFMI TAEQYQIVGG HLLATIDELL SPGQEVLDAW 

       130        140        150        160        170        180 
GEAYGVLANV FITREEAIYQ ENEEKTGGWR GTREFTLIEK TQESDVITSF TFKPADGGTV 

       190        200        210        220        230        240 
SNFKPGQYLG IYLTPDAFEF QEIRQYSLSS APRTDNYRIS VKREEGGKVS NYLHNELTVG 

       250        260        270        280        290        300 
DTVQLAAPAG DFFLDVPATT PVTLISAGVG LTPTLSMLDA LTEHQASVHW LHATENGSHH 

       310        320        330        340        350        360 
AFKDYVNTLA DKYDHISATT WYREPLATDR PAEDFDYQGM IDLKAVASQL IDPAMHYYFC 

       370        380        390 
GPVGFMQHVA KQLIELDVQE DNIHYECFGP HKVL

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References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	CR378673 Genomic DNA. Translation: CAG21641.1.
RefSeq	YP_131443.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3124922.
GenomeReviews	Gene locus PBPRA3343 in contig CR354531_GR.
KEGG	ppr:PBPRA3343.
NMPDR	fig\|298386.1.peg.5549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LM37.
OMA	KHRSLGI.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRA3343-MON.
BRENDA	1.14.12.17. 263915.
Family and domain databases
HAMAP	MF_01252. [Tree]
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012292. Globin. IPR000971. Globin_subset. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR001221. Phe_hydroxylase. [Graphical view]
Gene3D	G3DSA:1.10.490.10. Globin_related. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00042. Globin. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00410. PHEHYDRXLASE.
PROSITE	PS51384. FAD_FR. 1 hit. PS01033. GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

HMP_PHOPR

Accession

Primary (citable) accession number: Q6LM37

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents