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Protein

Beta-galactosidase

Gene

lacZ

Organism
Photobacterium profundum (strain SS9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99SubstrateUniRule annotation1
Metal bindingi197SodiumUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Sitei352Transition state stabilizerUniRule annotation1
Sitei386Transition state stabilizerUniRule annotation1
Metal bindingi411Magnesium 1UniRule annotation1
Metal bindingi413Magnesium 1UniRule annotation1
Active sitei456Proton donorUniRule annotation1
Metal bindingi456Magnesium 1UniRule annotation1
Binding sitei456SubstrateUniRule annotation1
Active sitei532NucleophileUniRule annotation1
Metal bindingi592Magnesium 2UniRule annotation1
Metal bindingi596Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi599SodiumUniRule annotation1
Binding sitei599SubstrateUniRule annotation1
Binding sitei1004SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMagnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:PBPRB0034
OrganismiPhotobacterium profundum (strain SS9)
Taxonomic identifieri298386 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000000593 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670051 – 1030Beta-galactosidaseAdd BLAST1030

Proteomic databases

PRIDEiQ6LL68

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRB0034

Structurei

3D structure databases

ProteinModelPortaliQ6LL68
SMRiQ6LL68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni532 – 535Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000252443
KOiK01190
OMAiPSNWQLQ
OrthoDBiPOG091H07QB

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6LL68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSDIIQRR DWENPQSVNI HCLKAHSPLA SYRDINHARD GIHAQRQSLN
60 70 80 90 100
GQWKFKLFDA PEQVEGEFID VQFNDSAWGD ITVPSNWQLQ GYDKPIYANV
110 120 130 140 150
KYPFEVNPPY VPADNPTGCY RTRLTLTEAD LESTQRIIFD GVNSAFHLWC
160 170 180 190 200
NGDWVGYSQD SRLPAEFDLS QYLTAGENTL AVMVIRWSDG SYLEDQDMWW
210 220 230 240 250
LSGIFRDVTL LSKPKQCIED VFITPDLDAC YRDGSLSIVT HISAPETSQV
260 270 280 290 300
HVQLFDGSQA VTEPSIARPH NRRIDERGSY DDVVFQTLHV REPQQWTAET
310 320 330 340 350
PNLYRVVVSL LDAEGNHLES EAYQVGFRKV EVKDGQLQLN GKPLLIRGVN
360 370 380 390 400
RHEHHPELGH VMTEEDMVRD ICLMKQYNFN AVRTAHYPNH PRWYELCDQY
410 420 430 440 450
GLYVCDEANI ETHGMIPMNR LSADPQWAHA YMSRYTQMVM RDKNHPSIII
460 470 480 490 500
WSLGNESGHG SSHNAMYAWS KQFDPSRPVQ YEGGGANTTA TDIICPMYAR
510 520 530 540 550
VNTTVEDEAV PKWPIKQWIS LPNEQRPLIL CEYAHAMGNS LGNFNEYWDA
560 570 580 590 600
FREFPRLQGG FIWDWVDQGL SQWDNDGKHF WAYGGDFGDT INDRQFCING
610 620 630 640 650
LIFPDRTPHP TLEEVKFCQR MITVALTQQD KQQCHLTVTN EYVFRSTDNE
660 670 680 690 700
QLHWSVLENG VEVQSGQCTL AIDAGSQQTV DIALDFQPKA DAKYHLNTDI
710 720 730 740 750
CLISATPWAQ AGHVSATEQF TLSNTSSLTL PKISILSAPQ LSEQGRDILV
760 770 780 790 800
SNLDKKHQWQ WNVESGLLTS WMVDGQSQLL HAPEDNFFRA PLDNDIGVSE
810 820 830 840 850
IDNIDPNAWV CRWDAAGIGR WERECVSCTS ESLSQAVKVT STFAYHHNGG
860 870 880 890 900
VQAITVWTYT LDNQGEMHID VDVTLADHLP PMPRIGLELA LPLPSDNTTV
910 920 930 940 950
TWQGLGPFEN YPDRLAAARF GQHTQSLDAM HTPYIFPTDS GLRSGTQWLN
960 970 980 990 1000
VGNLECTGDF LFSVSRFSQQ QLTEAKHTNE LTLEDKIYLR IDHQHMGVGG
1010 1020 1030
DDSWSPSVHE EFQLTDNTYR FSIMLKPRHN
Length:1,030
Mass (Da):117,266
Last modified:July 5, 2004 - v1
Checksum:i6A859CAEA1AF7939
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378675 Genomic DNA Translation: CAG21907.1

Genome annotation databases

EnsemblBacteriaiCAG21907; CAG21907; PBPRB0034
KEGGippr:PBPRB0034

Similar proteinsi

Entry informationi

Entry nameiBGAL_PHOPR
AccessioniPrimary (citable) accession number: Q6LL68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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