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Protein

Beta-galactosidase

Gene

lacZ

Organism
Photobacterium profundum (strain SS9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99SubstrateUniRule annotation1
Metal bindingi197SodiumUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Sitei352Transition state stabilizerUniRule annotation1
Sitei386Transition state stabilizerUniRule annotation1
Metal bindingi411Magnesium 1UniRule annotation1
Metal bindingi413Magnesium 1UniRule annotation1
Active sitei456Proton donorUniRule annotation1
Metal bindingi456Magnesium 1UniRule annotation1
Binding sitei456SubstrateUniRule annotation1
Active sitei532NucleophileUniRule annotation1
Metal bindingi592Magnesium 2UniRule annotation1
Metal bindingi596Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi599SodiumUniRule annotation1
Binding sitei599SubstrateUniRule annotation1
Binding sitei1004SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:PBPRB0034
OrganismiPhotobacterium profundum (strain SS9)
Taxonomic identifieri298386 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000000593 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670051 – 1030Beta-galactosidaseAdd BLAST1030

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi298386.PBPRB0034.

Structurei

3D structure databases

ProteinModelPortaliQ6LL68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni532 – 535Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDIETRMY.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LL68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSDIIQRR DWENPQSVNI HCLKAHSPLA SYRDINHARD GIHAQRQSLN
60 70 80 90 100
GQWKFKLFDA PEQVEGEFID VQFNDSAWGD ITVPSNWQLQ GYDKPIYANV
110 120 130 140 150
KYPFEVNPPY VPADNPTGCY RTRLTLTEAD LESTQRIIFD GVNSAFHLWC
160 170 180 190 200
NGDWVGYSQD SRLPAEFDLS QYLTAGENTL AVMVIRWSDG SYLEDQDMWW
210 220 230 240 250
LSGIFRDVTL LSKPKQCIED VFITPDLDAC YRDGSLSIVT HISAPETSQV
260 270 280 290 300
HVQLFDGSQA VTEPSIARPH NRRIDERGSY DDVVFQTLHV REPQQWTAET
310 320 330 340 350
PNLYRVVVSL LDAEGNHLES EAYQVGFRKV EVKDGQLQLN GKPLLIRGVN
360 370 380 390 400
RHEHHPELGH VMTEEDMVRD ICLMKQYNFN AVRTAHYPNH PRWYELCDQY
410 420 430 440 450
GLYVCDEANI ETHGMIPMNR LSADPQWAHA YMSRYTQMVM RDKNHPSIII
460 470 480 490 500
WSLGNESGHG SSHNAMYAWS KQFDPSRPVQ YEGGGANTTA TDIICPMYAR
510 520 530 540 550
VNTTVEDEAV PKWPIKQWIS LPNEQRPLIL CEYAHAMGNS LGNFNEYWDA
560 570 580 590 600
FREFPRLQGG FIWDWVDQGL SQWDNDGKHF WAYGGDFGDT INDRQFCING
610 620 630 640 650
LIFPDRTPHP TLEEVKFCQR MITVALTQQD KQQCHLTVTN EYVFRSTDNE
660 670 680 690 700
QLHWSVLENG VEVQSGQCTL AIDAGSQQTV DIALDFQPKA DAKYHLNTDI
710 720 730 740 750
CLISATPWAQ AGHVSATEQF TLSNTSSLTL PKISILSAPQ LSEQGRDILV
760 770 780 790 800
SNLDKKHQWQ WNVESGLLTS WMVDGQSQLL HAPEDNFFRA PLDNDIGVSE
810 820 830 840 850
IDNIDPNAWV CRWDAAGIGR WERECVSCTS ESLSQAVKVT STFAYHHNGG
860 870 880 890 900
VQAITVWTYT LDNQGEMHID VDVTLADHLP PMPRIGLELA LPLPSDNTTV
910 920 930 940 950
TWQGLGPFEN YPDRLAAARF GQHTQSLDAM HTPYIFPTDS GLRSGTQWLN
960 970 980 990 1000
VGNLECTGDF LFSVSRFSQQ QLTEAKHTNE LTLEDKIYLR IDHQHMGVGG
1010 1020 1030
DDSWSPSVHE EFQLTDNTYR FSIMLKPRHN
Length:1,030
Mass (Da):117,266
Last modified:July 5, 2004 - v1
Checksum:i6A859CAEA1AF7939
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378675 Genomic DNA. Translation: CAG21907.1.

Genome annotation databases

EnsemblBacteriaiCAG21907; CAG21907; PBPRB0034.
KEGGippr:PBPRB0034.
PATRICi22938410. VBIPhoPro109272_3773.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR378675 Genomic DNA. Translation: CAG21907.1.

3D structure databases

ProteinModelPortaliQ6LL68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298386.PBPRB0034.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG21907; CAG21907; PBPRB0034.
KEGGippr:PBPRB0034.
PATRICi22938410. VBIPhoPro109272_3773.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDIETRMY.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_PHOPR
AccessioniPrimary (citable) accession number: Q6LL68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: October 5, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.