Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q6LL68 (BGAL_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:PBPRB0034
OrganismPhotobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]
Taxonomic identifier74109 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length1030 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687

Subunit structure

Homotetramer By similarity. HAMAP MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10301030Beta-galactosidase HAMAP MF_01687
PRO_0000367005

Regions

Region532 – 5354Substrate binding By similarity

Sites

Active site4561Proton donor By similarity
Active site5321Nucleophile By similarity
Metal binding1971Sodium By similarity
Metal binding4111Magnesium 1 By similarity
Metal binding4131Magnesium 1 By similarity
Metal binding4561Magnesium 1 By similarity
Metal binding5921Magnesium 2 By similarity
Metal binding5961Sodium; via carbonyl oxygen By similarity
Metal binding5991Sodium By similarity
Binding site991Substrate By similarity
Binding site1971Substrate By similarity
Binding site4561Substrate By similarity
Binding site5991Substrate By similarity
Binding site10041Substrate By similarity
Site3521Transition state stabilizer By similarity
Site3861Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6LL68 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 6A859CAEA1AF7939

FASTA1,030117,266
        10         20         30         40         50         60 
MALSDIIQRR DWENPQSVNI HCLKAHSPLA SYRDINHARD GIHAQRQSLN GQWKFKLFDA 

        70         80         90        100        110        120 
PEQVEGEFID VQFNDSAWGD ITVPSNWQLQ GYDKPIYANV KYPFEVNPPY VPADNPTGCY 

       130        140        150        160        170        180 
RTRLTLTEAD LESTQRIIFD GVNSAFHLWC NGDWVGYSQD SRLPAEFDLS QYLTAGENTL 

       190        200        210        220        230        240 
AVMVIRWSDG SYLEDQDMWW LSGIFRDVTL LSKPKQCIED VFITPDLDAC YRDGSLSIVT 

       250        260        270        280        290        300 
HISAPETSQV HVQLFDGSQA VTEPSIARPH NRRIDERGSY DDVVFQTLHV REPQQWTAET 

       310        320        330        340        350        360 
PNLYRVVVSL LDAEGNHLES EAYQVGFRKV EVKDGQLQLN GKPLLIRGVN RHEHHPELGH 

       370        380        390        400        410        420 
VMTEEDMVRD ICLMKQYNFN AVRTAHYPNH PRWYELCDQY GLYVCDEANI ETHGMIPMNR 

       430        440        450        460        470        480 
LSADPQWAHA YMSRYTQMVM RDKNHPSIII WSLGNESGHG SSHNAMYAWS KQFDPSRPVQ 

       490        500        510        520        530        540 
YEGGGANTTA TDIICPMYAR VNTTVEDEAV PKWPIKQWIS LPNEQRPLIL CEYAHAMGNS 

       550        560        570        580        590        600 
LGNFNEYWDA FREFPRLQGG FIWDWVDQGL SQWDNDGKHF WAYGGDFGDT INDRQFCING 

       610        620        630        640        650        660 
LIFPDRTPHP TLEEVKFCQR MITVALTQQD KQQCHLTVTN EYVFRSTDNE QLHWSVLENG 

       670        680        690        700        710        720 
VEVQSGQCTL AIDAGSQQTV DIALDFQPKA DAKYHLNTDI CLISATPWAQ AGHVSATEQF 

       730        740        750        760        770        780 
TLSNTSSLTL PKISILSAPQ LSEQGRDILV SNLDKKHQWQ WNVESGLLTS WMVDGQSQLL 

       790        800        810        820        830        840 
HAPEDNFFRA PLDNDIGVSE IDNIDPNAWV CRWDAAGIGR WERECVSCTS ESLSQAVKVT 

       850        860        870        880        890        900 
STFAYHHNGG VQAITVWTYT LDNQGEMHID VDVTLADHLP PMPRIGLELA LPLPSDNTTV 

       910        920        930        940        950        960 
TWQGLGPFEN YPDRLAAARF GQHTQSLDAM HTPYIFPTDS GLRSGTQWLN VGNLECTGDF 

       970        980        990       1000       1010       1020 
LFSVSRFSQQ QLTEAKHTNE LTLEDKIYLR IDHQHMGVGG DDSWSPSVHE EFQLTDNTYR 

      1030 
FSIMLKPRHN

« Hide

References

[1]"Life at depth: Photobacterium profundum genome sequence and expression analysis."
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.
Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SS9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR378675 Genomic DNA. Translation: CAG21907.1.
RefSeqYP_131707.1. NC_006371.1.

3D structure databases

HSSPHSSP built from PDB template 1PX3 based on UniProtKB P00722.
ProteinModelPortalQ6LL68.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3120206.
GenomeReviewsGene locus PBPRB0034 in contig CR354532_GR.
KEGGppr:PBPRB0034.
NMPDRfig|298386.1.peg.38.
PATRIC22938410. VBIPhoPro109272_3773.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG670643.
OMARDWENPA.
PhylomeDBQ6LL68.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycPPRO298386:PBPRB0034-MONOMER.

Family and domain databases

HAMAPMF_01687. Beta_gal.
[Tree]
InterProIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK01190.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. False negative.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_PHOPR
AccessionPrimary (citable) accession number: Q6LL68
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents