Q6LHY2 (SYC2_PHOPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cysteine--tRNA ligase 2
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase 2
Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	PBPRB1226

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Putative cysteine--tRNA ligase 2 HAMAP MF_00041		PRO_0000159454
Sites
	Metal binding	32	1	Zinc By similarity
	Metal binding	223	1	Zinc By similarity
	Metal binding	248	1	Zinc By similarity
	Metal binding	252	1	Zinc By similarity
	Binding site	283	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LHY2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EC931FE165B1B0CA	FASTA	457	52,294
	10 20 30 40 50 60 MSQPELLLFD TMARELRPFN SIRANKVGLY ACGPTVYDYA HAGNLRTYLF VDVLRRTLEI 70 80 90 100 110 120 NGYEVNHVMN ITDVGHLVSD ADTGEDKMEK GARKQNKSAW EIAQFFEQAF FDDLKLLNIS 130 140 150 160 170 180 TPSVTCRATE HIQEQIKFIQ ELETKGFTYQ TSDGVYFNTD RLADYGKLAR LDKQGLEAGI 190 200 210 220 230 240 RVEMAEKKHP TDFALWKFSG DKPRQMEWQG PWGIGFPGWH IECSAMAEKY LGDVFDIHVG 250 260 270 280 290 300 GEDHIPVHHT NEIAQCQAKN GHVQANYWLH GYFLQLKKEK ISKSGTSLRL DALVAKGYEP 310 320 330 340 350 360 MAYRYLTLTS HYRSHLSFTW EGLSGAQKAL HRLRNKVAFL PSNGNVDESY RELFMRHINR 370 380 390 400 410 420 DLNMPQALAL VWDVLNSELL PENKRATALF FDRILGLDIH KIETVEIPEH ISRLVELRTQ 430 440 450 VKRQGNFKKA DAIRNQIHDL GYQVNDSGDG STVTIRS

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR378678 Genomic DNA. Translation: CAG23098.1.
RefSeq	YP_132898.1. NC_006371.1.
3D structure databases
ProteinModelPortal	Q6LHY2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3121512.
GenomeReviews	Gene locus PBPRB1226 in contig CR354532_GR.
KEGG	ppr:PBPRB1226.
NMPDR	fig|298386.1.peg.1319.
PATRIC	22941048. VBIPhoPro109272_5090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	IGNLRTY.
PhylomeDB	Q6LHY2.
ProtClustDB	CLSK2395547.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRB1226-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. Divergent sequence. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_PHOPR

Accession

Primary (citable) accession number: Q6LHY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program