Reviewed, UniProtKB/Swiss-Prot Q6LHK5 (MAO12_PHOPR)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme 2
      Short name=NAD-ME 2
    EC=1.1.1.38

Gene names

Name:	sfcA2
Ordered Locus Names:	PBPRB1354

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	NAD-dependent malic enzyme 2 HAMAP MF_01619		PRO_0000160222
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	411	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LHK5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 19D7E3B15F06EC81	FASTA	558	61,562
	10 20 30 40 50 60 MYENNKTLYL PYAGPTLLEN ALLNKGSAFS PEERQNFSLM GLLPAAIESI TEQEERAYSQ 70 80 90 100 110 120 YQTFNDDMDK HIYLRNIQDT NETLYYRLID NHIEEMMPII YTPTVGAACE QFSNIYRRGR 130 140 150 160 170 180 GLFLGYPDKG NIVDILNNAA RQDVKIIVIT DGERILGLGD QGIGGMGIPI GKLALYTACG 190 200 210 220 230 240 GINPANTLPI VLDVGTNNTQ LLSDPMYMGW RHPRITGQEY DDFVEEFIQA VKSRWPNALI 250 260 270 280 290 300 QFEDFAQKNA MPLLNRYKDK VCCFNDDIQG TAAVTVGSLL AACKAAGSEL SEQRITFVGA 310 320 330 340 350 360 GSAGCGIAEA IVAQMIAEGL SDSAARARIF MVDRWGLLTD NMQNLLDFQQ KLAQKSATVS 370 380 390 400 410 420 QWNETGNISL LDVVSNGKPT VLIGVPGLFS QEVIMEMHAH CKRPIVLPLS NPTSRVEATP 430 440 450 460 470 480 SDIIRWTEGD ALIATGSPFD PVIFNEKTYP IAQCNNSYIF PGIGLGVLAS GATRVTDEML 490 500 510 520 530 540 MESSRVLAEC SPLAQNGNGA LLPPLKDIHQ VSHCIALAVA KKAVEQNKAP QRTEKQLLEK 550 IESYFWKPEY LKYKRTAL

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378679 Genomic DNA. Translation: CAG23225.1.
RefSeq	YP_133025.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3121452.
GenomeReviews	Gene locus PBPRB1354 in contig CR354532_GR.
KEGG	ppr:PBPRB1354.
NMPDR	fig|298386.1.peg.1460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LHK5.
OMA	Q6LHK5. AECNNST.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRB1354-MON.
BRENDA	1.1.1.38. 263915.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO12_PHOPR

Accession

Primary (citable) accession number: Q6LHK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)