Reviewed, UniProtKB/Swiss-Prot Q6LGK4 (GUAC_PHOPR)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	PBPRB1716

Organism

Photobacterium profundum (Photobacterium sp. (strain SS9)) [Complete proteome] [HAMAP]

Taxonomic identifier

74109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH. HAMAP MF_00596
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	GMP reductase HAMAP MF_00596		PRO_1000025615
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP; ribose moiety By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6LGK4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0638BB1EE360BF83	FASTA	347	37,374
	10 20 30 40 50 60 MRIEQDLKLG FKDVLFRPKR STLKSRSQVE LTRDFTFKHS GRQWSGVPII AANMDSVGSF 70 80 90 100 110 120 EMAASLSKHN VMTAIHKHYT VDDWAGFVKN NDAAVLNNAM VSTGTSEADF QKTKDIMALT 130 140 150 160 170 180 EDLIFICIDI ANGYSEHLVE YVQKVRAEFP TKVISAGNVV TGDMVEELIL AGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRV KTGVGYPQLS AIIECSDAAH GLGGRIIGDG GCSCAGDVSK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHEE SNGEVIEKDG EMFMKFYGMS SQSAMDKHSG GVANYRAAEG KTVLLPFRGP 310 320 330 340 VENTIQDIMG GIRSTCTYVG AAQLKELTKR ATFIRVQEQE NNVYGKE

References

[1]

"Life at depth: Photobacterium profundum genome sequence and expression analysis." Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G. Science 307:1459-1461(2005) [PubMed: 15746425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR378680 Genomic DNA. Translation: CAG23576.1.
RefSeq	YP_133376.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
ModBase	Search...
Genome annotation databases
GeneID	3121815.
GenomeReviews	Gene locus PBPRB1716 in contig CR354532_GR.
KEGG	ppr:PBPRB1716.
NMPDR	fig|298386.1.peg.1838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6LGK4.
Enzyme and pathway databases
BioCyc	PPRO298386:PBPRB1716-MON.
Family and domain databases
HAMAP	MF_00596. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_PHOPR

Accession

Primary (citable) accession number: Q6LGK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)