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Q6LDD9

- HPRT_MACFA

UniProt

Q6LDD9 - HPRT_MACFA

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Protein
Hypoxanthine-guanine phosphoribosyltransferase
Gene
HPRT1, HPRT, QbsA-10852, QflA-10339
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691GMP By similarity
Active sitei138 – 1381Proton acceptor By similarity
Binding sitei166 – 1661GMP By similarity
Metal bindingi194 – 1941Magnesium By similarity
Binding sitei194 – 1941GMP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429GMP By similarity
Nucleotide bindingi186 – 1883GMP By similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB
  4. nucleotide binding Source: UniProtKB-KW
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. GMP catabolic process Source: UniProtKB
  2. IMP metabolic process Source: UniProtKB
  3. IMP salvage Source: UniProtKB-UniPathway
  4. guanine salvage Source: UniProtKB
  5. hypoxanthine metabolic process Source: UniProtKB
  6. hypoxanthine salvage Source: UniProtKB
  7. positive regulation of dopamine metabolic process Source: UniProtKB
  8. protein homotetramerization Source: UniProtKB
  9. purine nucleotide biosynthetic process Source: UniProtKB
  10. purine ribonucleoside salvage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HPRT1
Synonyms:HPRT
ORF Names:QbsA-10852, QflA-10339
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei103 – 1031N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ6LDD9.

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6LDD9.
SMRiQ6LDD9. Positions 5-218.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG000242.
KOiK00760.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6LDD9-1 [UniParc]FASTAAdd to Basket

« Hide

MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA    50
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI 100
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV 150
RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR 200
DLNHVCVISE TGKAKYKA 218
Length:218
Mass (Da):24,579
Last modified:January 23, 2007 - v3
Checksum:i1928EE69517CCB40
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561E → K in BAE01621. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S43335 mRNA. Translation: AAD13829.1.
AB125173 mRNA. Translation: BAD51961.1.
AB169539 mRNA. Translation: BAE01621.1.
RefSeqiNP_001270523.1. NM_001283594.1.
UniGeneiMfa.2108.

Genome annotation databases

GeneIDi101867079.
KEGGimcf:101867079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S43335 mRNA. Translation: AAD13829.1 .
AB125173 mRNA. Translation: BAD51961.1 .
AB169539 mRNA. Translation: BAE01621.1 .
RefSeqi NP_001270523.1. NM_001283594.1.
UniGenei Mfa.2108.

3D structure databases

ProteinModelPortali Q6LDD9.
SMRi Q6LDD9. Positions 5-218.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q6LDD9.

Proteomic databases

PRIDEi Q6LDD9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101867079.
KEGGi mcf:101867079.

Organism-specific databases

CTDi 3251.

Phylogenomic databases

HOVERGENi HBG000242.
KOi K00760.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes."
    Harbach P.R., Filipunas A.L., Wang Y., Aaron C.S.
    Environ. Mol. Mutagen. 20:96-105(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of cDNA for macaque neurological disease genes."
    Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain stem.
  3. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Frontal cortex.

Entry informationi

Entry nameiHPRT_MACFA
AccessioniPrimary (citable) accession number: Q6LDD9
Secondary accession number(s): Q4R5K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 61 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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