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Q6LDD9

- HPRT_MACFA

UniProt

Q6LDD9 - HPRT_MACFA

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691GMPBy similarity
    Active sitei138 – 1381Proton acceptorBy similarity
    Binding sitei166 – 1661GMPBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 1429GMPBy similarity
    Nucleotide bindingi186 – 1883GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
    3. magnesium ion binding Source: UniProtKB
    4. nucleotide binding Source: UniProtKB-KW
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. GMP catabolic process Source: UniProtKB
    2. guanine salvage Source: UniProtKB
    3. hypoxanthine metabolic process Source: UniProtKB
    4. hypoxanthine salvage Source: UniProtKB
    5. IMP metabolic process Source: UniProtKB
    6. IMP salvage Source: UniProtKB-UniPathway
    7. positive regulation of dopamine metabolic process Source: UniProtKB
    8. protein homotetramerization Source: UniProtKB
    9. purine nucleotide biosynthetic process Source: UniProtKB
    10. purine ribonucleoside salvage Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:HPRT1
    Synonyms:HPRT
    ORF Names:QbsA-10852, QflA-10339
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139586Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei103 – 1031N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ6LDD9.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LDD9.
    SMRiQ6LDD9. Positions 5-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOVERGENiHBG000242.
    KOiK00760.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6LDD9-1 [UniParc]FASTAAdd to Basket

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    MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA    50
    RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI 100
    RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV 150
    RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR 200
    DLNHVCVISE TGKAKYKA 218
    Length:218
    Mass (Da):24,579
    Last modified:January 23, 2007 - v3
    Checksum:i1928EE69517CCB40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561E → K in BAE01621. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43335 mRNA. Translation: AAD13829.1.
    AB125173 mRNA. Translation: BAD51961.1.
    AB169539 mRNA. Translation: BAE01621.1.
    RefSeqiNP_001270523.1. NM_001283594.1.
    UniGeneiMfa.2108.

    Genome annotation databases

    GeneIDi101867079.
    KEGGimcf:101867079.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43335 mRNA. Translation: AAD13829.1 .
    AB125173 mRNA. Translation: BAD51961.1 .
    AB169539 mRNA. Translation: BAE01621.1 .
    RefSeqi NP_001270523.1. NM_001283594.1.
    UniGenei Mfa.2108.

    3D structure databases

    ProteinModelPortali Q6LDD9.
    SMRi Q6LDD9. Positions 5-218.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q6LDD9.

    Proteomic databases

    PRIDEi Q6LDD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101867079.
    KEGGi mcf:101867079.

    Organism-specific databases

    CTDi 3251.

    Phylogenomic databases

    HOVERGENi HBG000242.
    KOi K00760.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes."
      Harbach P.R., Filipunas A.L., Wang Y., Aaron C.S.
      Environ. Mol. Mutagen. 20:96-105(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of cDNA for macaque neurological disease genes."
      Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain stem.
    3. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Frontal cortex.

    Entry informationi

    Entry nameiHPRT_MACFA
    AccessioniPrimary (citable) accession number: Q6LDD9
    Secondary accession number(s): Q4R5K4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3