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Q6LBA6 (RBL_OLICO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain

Short name=RuBisCO large subunit
EC=4.1.1.39
Gene names
Name:cbbL
Ordered Locus Names:OCA5_pHCG300470
Encoded onPlasmid megaplasmid pHCG3
OrganismOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]
Taxonomic identifier504832 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Induction

Expressed under CO2 and H2 autotrophic, but not under heterotrophic growth conditions. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338
PRO_0000062635

Sites

Active site1771Proton acceptor By similarity
Active site2951Proton acceptor By similarity
Metal binding2031Magnesium; via carbamate group By similarity
Metal binding2051Magnesium By similarity
Metal binding2061Magnesium By similarity
Binding site1251Substrate; in homodimeric partner By similarity
Binding site1751Substrate By similarity
Binding site1791Substrate By similarity
Binding site2961Substrate By similarity
Binding site3281Substrate By similarity
Binding site3801Substrate By similarity
Site3351Transition state stabilizer By similarity

Amino acid modifications

Modified residue2031N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6LBA6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B200320194573F67

FASTA48653,763
        10         20         30         40         50         60 
MNDQSMTIRG KDRYKSGVMA YKKMGYWEPD YVPKDTDVIA LFRVTPQDGV DPIEAAAAVA 

        70         80         90        100        110        120 
GESSTATWTV VWTDRLTAAE KYRAKCYRVD PVPNSPGQYF AYIAYDLDLF EPGSISNLTA 

       130        140        150        160        170        180 
SIIGNVFGFK PLKGLRLEDM RLPVAYVKTF QGPATGIVVE RERLDKFGRP LLGATVKPKL 

       190        200        210        220        230        240 
GLSGRNYGRV VYEALKGGLD FTKDDENINS QPFMHWRERF LYCMEAVNRA QAASGEVKGT 

       250        260        270        280        290        300 
YLNVTAATME DMYERAEFAK ELGSCIVMID LVIGYTAIQS MAKWARKNDM ILHLHRAGHS 

       310        320        330        340        350        360 
TYTRQKNHGV SFRVIAKWMR LAGVDHIHAG TVVGKLEGDP NTTRGYYDIC REEFNPTKLE 

       370        380        390        400        410        420 
HGIFFDQNWA SLNKMMPVAS GGIHAGQMHQ LLDLLGEDVV LQFGGGTIGH PMGIQAGAIA 

       430        440        450        460        470        480 
NRVALEAMIL ARNEGRDYVA EGPEILAKAA ATCTPLKSAL EVWKDVTFNY ESTDAPDFVP 


TAIAAV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis, characterization and CO-specific transcription of the cox gene cluster on the megaplasmid pHCG3 of Oligotropha carboxidovorans."
Santiago B., Schuebel U., Egelseer C., Meyer O.
Gene 236:115-124(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MRNA EXPRESSION.
Strain: ATCC 49405 / DSM 1227 / OM5.
[2]"Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H(2) and CO(2)."
Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O.
Gene 322:67-75(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.
[3]"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002827 Genomic DNA. Translation: AEI08121.1.
RefSeqYP_004638448.1. NC_015689.1.

3D structure databases

ProteinModelPortalQ6LBA6.
SMRQ6LBA6. Positions 13-479.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEI08121; AEI08121; OCA5_pHCG300470.
GeneID10852798.
KEGGocg:OCA5_pHCG300470.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01601.
OMAHRAMHAA.
ProtClustDBPRK04208.

Enzyme and pathway databases

BioCycOCAR504832:GJPZ-3614-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBL_OLICO
AccessionPrimary (citable) accession number: Q6LBA6
Secondary accession number(s): F8C111
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families