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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125Substrate; in homodimeric partnerUniRule annotation1
Binding sitei175SubstrateUniRule annotation1
Active sitei177Proton acceptorUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Metal bindingi203Magnesium; via carbamate groupUniRule annotation1
Metal bindingi205MagnesiumUniRule annotation1
Metal bindingi206MagnesiumUniRule annotation1
Active sitei295Proton acceptorUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei328SubstrateUniRule annotation1
Sitei335Transition state stabilizerUniRule annotation1
Binding sitei380SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Ordered Locus Names:OCA5_pHCG300470
Encoded oniPlasmid megaplasmid pHCG30 Publication
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
Proteomesi
  • UP000007730 Componenti: Plasmid pHCG3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000626351 – 486Ribulose bisphosphate carboxylase large chainAdd BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203N6-carboxylysineUniRule annotation1

Expressioni

Inductioni

Expressed under CO2 and H2 autotrophic, but not under heterotrophic growth conditions.

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6LBA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6LBA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDQSMTIRG KDRYKSGVMA YKKMGYWEPD YVPKDTDVIA LFRVTPQDGV
60 70 80 90 100
DPIEAAAAVA GESSTATWTV VWTDRLTAAE KYRAKCYRVD PVPNSPGQYF
110 120 130 140 150
AYIAYDLDLF EPGSISNLTA SIIGNVFGFK PLKGLRLEDM RLPVAYVKTF
160 170 180 190 200
QGPATGIVVE RERLDKFGRP LLGATVKPKL GLSGRNYGRV VYEALKGGLD
210 220 230 240 250
FTKDDENINS QPFMHWRERF LYCMEAVNRA QAASGEVKGT YLNVTAATME
260 270 280 290 300
DMYERAEFAK ELGSCIVMID LVIGYTAIQS MAKWARKNDM ILHLHRAGHS
310 320 330 340 350
TYTRQKNHGV SFRVIAKWMR LAGVDHIHAG TVVGKLEGDP NTTRGYYDIC
360 370 380 390 400
REEFNPTKLE HGIFFDQNWA SLNKMMPVAS GGIHAGQMHQ LLDLLGEDVV
410 420 430 440 450
LQFGGGTIGH PMGIQAGAIA NRVALEAMIL ARNEGRDYVA EGPEILAKAA
460 470 480
ATCTPLKSAL EVWKDVTFNY ESTDAPDFVP TAIAAV
Length:486
Mass (Da):53,763
Last modified:July 5, 2004 - v1
Checksum:iB200320194573F67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08121.1.
RefSeqiWP_013913745.1. NC_015689.1.

Genome annotation databases

EnsemblBacteriaiAEI08121; AEI08121; OCA5_pHCG300470.
KEGGiocg:OCA5_pHCG300470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08121.1.
RefSeqiWP_013913745.1. NC_015689.1.

3D structure databases

ProteinModelPortaliQ6LBA6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI08121; AEI08121; OCA5_pHCG300470.
KEGGiocg:OCA5_pHCG300470.

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBL_OLICO
AccessioniPrimary (citable) accession number: Q6LBA6
Secondary accession number(s): F8C111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.