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Q6LBA6

- RBL_OLICO

UniProt

Q6LBA6 - RBL_OLICO

Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251Substrate; in homodimeric partnerUniRule annotation
    Binding sitei175 – 1751SubstrateUniRule annotation
    Active sitei177 – 1771Proton acceptorUniRule annotation
    Binding sitei179 – 1791SubstrateUniRule annotation
    Metal bindingi203 – 2031Magnesium; via carbamate groupUniRule annotation
    Metal bindingi205 – 2051MagnesiumUniRule annotation
    Metal bindingi206 – 2061MagnesiumUniRule annotation
    Active sitei295 – 2951Proton acceptorUniRule annotation
    Binding sitei296 – 2961SubstrateUniRule annotation
    Binding sitei328 – 3281SubstrateUniRule annotation
    Sitei335 – 3351Transition state stabilizerUniRule annotation
    Binding sitei380 – 3801SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. monooxygenase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. reductive pentose-phosphate cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Calvin cycle, Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciOCAR504832:GJPZ-3614-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCO large subunitUniRule annotation
    Gene namesi
    Name:cbbLUniRule annotation
    Ordered Locus Names:OCA5_pHCG300470
    Encoded oniPlasmid megaplasmid pHCG30 Publication
    OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
    Taxonomic identifieri504832 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
    ProteomesiUP000007730: Plasmid pHCG3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 486486Ribulose bisphosphate carboxylase large chainPRO_0000062635Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei203 – 2031N6-carboxylysineUniRule annotation

    Expressioni

    Inductioni

    Expressed under CO2 and H2 autotrophic, but not under heterotrophic growth conditions.

    Interactioni

    Subunit structurei

    Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ6LBA6.
    SMRiQ6LBA6. Positions 13-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.
    OMAiCTPLKQA.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01338. RuBisCO_L_type1.
    InterProiIPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6LBA6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDQSMTIRG KDRYKSGVMA YKKMGYWEPD YVPKDTDVIA LFRVTPQDGV    50
    DPIEAAAAVA GESSTATWTV VWTDRLTAAE KYRAKCYRVD PVPNSPGQYF 100
    AYIAYDLDLF EPGSISNLTA SIIGNVFGFK PLKGLRLEDM RLPVAYVKTF 150
    QGPATGIVVE RERLDKFGRP LLGATVKPKL GLSGRNYGRV VYEALKGGLD 200
    FTKDDENINS QPFMHWRERF LYCMEAVNRA QAASGEVKGT YLNVTAATME 250
    DMYERAEFAK ELGSCIVMID LVIGYTAIQS MAKWARKNDM ILHLHRAGHS 300
    TYTRQKNHGV SFRVIAKWMR LAGVDHIHAG TVVGKLEGDP NTTRGYYDIC 350
    REEFNPTKLE HGIFFDQNWA SLNKMMPVAS GGIHAGQMHQ LLDLLGEDVV 400
    LQFGGGTIGH PMGIQAGAIA NRVALEAMIL ARNEGRDYVA EGPEILAKAA 450
    ATCTPLKSAL EVWKDVTFNY ESTDAPDFVP TAIAAV 486
    Length:486
    Mass (Da):53,763
    Last modified:July 5, 2004 - v1
    Checksum:iB200320194573F67
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002827 Genomic DNA. Translation: AEI08121.1.
    RefSeqiWP_013913745.1. NC_015689.1.
    YP_004638448.1. NC_015689.1.

    Genome annotation databases

    EnsemblBacteriaiAEI08121; AEI08121; OCA5_pHCG300470.
    GeneIDi10852798.
    KEGGiocg:OCA5_pHCG300470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002827 Genomic DNA. Translation: AEI08121.1 .
    RefSeqi WP_013913745.1. NC_015689.1.
    YP_004638448.1. NC_015689.1.

    3D structure databases

    ProteinModelPortali Q6LBA6.
    SMRi Q6LBA6. Positions 13-479.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEI08121 ; AEI08121 ; OCA5_pHCG300470 .
    GeneIDi 10852798.
    KEGGi ocg:OCA5_pHCG300470.

    Phylogenomic databases

    KOi K01601.
    OMAi CTPLKQA.

    Enzyme and pathway databases

    BioCyci OCAR504832:GJPZ-3614-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01338. RuBisCO_L_type1.
    InterProi IPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis, characterization and CO-specific transcription of the cox gene cluster on the megaplasmid pHCG3 of Oligotropha carboxidovorans."
      Santiago B., Schuebel U., Egelseer C., Meyer O.
      Gene 236:115-124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MRNA EXPRESSION.
      Strain: ATCC 49405 / DSM 1227 / OM5.
    2. "Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H(2) and CO(2)."
      Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O.
      Gene 322:67-75(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49405 / DSM 1227 / OM5.
    3. "Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
      Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
      J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49405 / DSM 1227 / OM5.

    Entry informationi

    Entry nameiRBL_OLICO
    AccessioniPrimary (citable) accession number: Q6LBA6
    Secondary accession number(s): F8C111
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3