Reviewed, UniProtKB/Swiss-Prot Q6L8S8 (B4GN3_MOUSE)

Names and origin

Protein names

Recommended name:
    N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 2
      Short name=NGalNAc-T2
    EC=2.4.1.244
Alternative name(s):
    Beta-1,4-N-acetylgalactosaminyltransferase III
      Short name=Beta4GalNAc-T3
      Short name=Beta4GalNAcT3

Gene names

Name:

B4galnt3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	986 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans. Mediates the N,N'-diacetyllactosediamine formation on gastric mucosa By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Localizes to apical Golgi By similarity.
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 986	986	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 2		PRO_0000252369
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 45	21	Signal-anchor for type II membrane protein Potential
	Topological domain	46 – 986	941	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6L8S8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D4CFB2B6B629A021	FASTA	986	113,509
	10 20 30 40 50 60 MGSPRAALLM LLLRPIKLLR RRFRLLLLLA VVSVGLWTLY LELVASAQAG GNPLNHRYGS 70 80 90 100 110 120 WRELAKALAS RNIPAVDPNL QFYRPQRLSL KDQEIARSRS RNSSYLKWNK PVPWLSEFRG 130 140 150 160 170 180 HANLHVFEDW CGSSIQQLRN NLHFPLYPHI RTTLRKLAVS PKWTNYGLRI FGYLHPFTDG 190 200 210 220 230 240 KIQFAIAADD NAEFWLSRDD QVSGLQLLAS VGKTGKEWTA PGEFGKFQSQ ISKPVSLSAS 250 260 270 280 290 300 LRYYFEVLHK QNDEGTDHVE VAWRRNDPGA KFTIIDSPFL SLFTNETILR MDEVGHIPQT 310 320 330 340 350 360 AASHVGSSNT PPRDEQPPAD MLRPDPRDTL FRVPLIAKSH LRHVLPDCPY KPSYLVDGLP 370 380 390 400 410 420 LQRYQGLRFV HLSFVYPNDY TRLSHMETHN KCFYQESAYD QDRSSFQEYI KMDKPEKHGP 430 440 450 460 470 480 EQPAGLEDGL LEESQYEDVP EEIPTSQDQN TGIQGRKQKT ISTPGLGVTD YHLRKLLARS 490 500 510 520 530 540 QSGPVAPLSK QNSTTAFPTR TSNIPVQRPE KSPVPSRDLS HSDQGARRNL PLIQRARPTG 550 560 570 580 590 600 DRPGKTLEQS QWLNQVESFI AEQRRGDRIE PPTPSRGWRP EEDVVIAADQ EGEVEEEEEG 610 620 630 640 650 660 EDEEEDMSEV FEYVPMFDPV VNWGQTFSAQ NLDFQALRTD WIDLNCNTSG NLLLPEQEAL 670 680 690 700 710 720 EVTRVFLRKL SQRTRGRYQL QRIVNVEKRQ DRLRGGRYFL ELELLDGQRL VRLSEYVSTR 730 740 750 760 770 780 GWRGGDHPGR EDTEARNLQG LVWSPRNRHR HVLNAQDPEP KLCWPQGFSW NHRAVVHFIV 790 800 810 820 830 840 PVKNQARWVQ QFIRDMESLS QVTGDAHFSI IITDYSSEDM DVEMALKRSR LRSYQYLKLS 850 860 870 880 890 900 GNFERSAGLQ AGIDLVKDPH SIIFLCDLHI HFPAGIIDTI RKHCVEGKMA FAPMVMRLHC 910 920 930 940 950 960 GATPQWPEGY WEVNGFGLLG IYKSDLDKIG GMNTKEFRDR WGGEDWELLD RILQAGLEVE 970 980 RLSLRNFFHH FHSKRGMWNR RQMKMP

References

[1]

"Molecular cloning and characterization of a novel human beta 1,4-N-acetylgalactosaminyltransferase, beta 4GalNAc-T3, responsible for the synthesis of N,N'-diacetyllactosediamine, galNAc beta 1-4GlcNAc." Sato T., Gotoh M., Kiyohara K., Kameyama A., Kubota T., Kikuchi N., Ishizuka Y., Iwasaki H., Togayachi A., Kudo T., Ohkura T., Nakanishi H., Narimatsu H. J. Biol. Chem. 278:47534-47544(2003) [PubMed: 12966086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

+

Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Beta1,4-N-acetylgalactosaminyltransferase III.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB114826 mRNA. Translation: BAD02450.1.
IPI	IPI00395175.
RefSeq	NP_942585.1.
UniGene	Mm.321840
3D structure databases
SMR	Q6L8S8. Positions 752-979.
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	Q6L8S8.
Genome annotation databases
Ensembl	ENSMUST00000057283; ENSMUSP00000058253; ENSMUSG00000041372; Mus musculus. [Genome view]
GeneID	330406.
KEGG	mmu:330406.
UCSC	uc009dmy.1. mouse.
Organism-specific databases
CTD	330406.
MGI	MGI:3041155. B4galnt3.
Phylogenomic databases
HOGENOM	HBG446821.
HOVERGEN	Q6L8S8.
InParanoid	Q6L8S8.
OMA	MALKRSK.
OrthoDB	EOG9GXJ6H.
PhylomeDB	Q6L8S8.
Enzyme and pathway databases
BRENDA	2.4.1.244. 244.
Gene expression databases
ArrayExpress	Q6L8S8.
Bgee	Q6L8S8.
CleanEx	MM_B4GALNT3.
Genevestigator	Q6L8S8.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. IPR011658. PA14. [Graphical view]
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
SMART	SM00758. PA14. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	399345.
SOURCE	Search...

Entry information

Entry name

B4GN3_MOUSE

Accession

Primary (citable) accession number: Q6L8S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)