Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2',5'-phosphodiesterase 12

Gene

PDE12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.4 Publications

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.3 Publications

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 8.0 for 2'-5' oligoadenylate exonuclease activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei351By similarity1
Metal bindingi351Magnesium 1By similarity1
Metal bindingi496Magnesium 2By similarity1
Metal bindingi498Magnesium 2By similarity1
Active sitei561By similarity1
Active sitei599By similarity1
Metal bindingi599Magnesium 2By similarity1

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: UniProtKB
  • exonuclease activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • poly(A)-specific ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  • cellular response to dsRNA Source: UniProtKB
  • cellular response to interferon-alpha Source: UniProtKB
  • cellular response to interferon-gamma Source: UniProtKB
  • mitochondrial mRNA catabolic process Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of cell death Source: UniProtKB
  • negative regulation of oxidative phosphorylation Source: UniProtKB
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
  • nucleic acid phosphodiester bond hydrolysis Source: UniProtKB
  • positive regulation of viral genome replication Source: UniProtKB
  • regulation of mitochondrial mRNA stability Source: UniProtKB
  • RNA phosphodiester bond hydrolysis, exonucleolytic Source: UniProtKB

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processmRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2',5'-phosphodiesterase 12 (EC:3.1.4.-)
Short name:
2'-PDE
Short name:
2-PDE
Alternative name(s):
Mitochondrial deadenylase (EC:3.1.13.4)
Gene namesi
Name:PDE12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000174840.8
HGNCiHGNC:25386 PDE12
MIMi616519 gene
neXtProtiNX_Q6L8Q7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi201626
OpenTargetsiENSG00000174840
PharmGKBiPA162399016

Polymorphism and mutation databases

BioMutaiPDE12
DMDMi172046137

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000032431243 – 6092',5'-phosphodiesterase 12Add BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei217PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6L8Q7
MaxQBiQ6L8Q7
PaxDbiQ6L8Q7
PeptideAtlasiQ6L8Q7
PRIDEiQ6L8Q7

PTM databases

iPTMnetiQ6L8Q7
PhosphoSitePlusiQ6L8Q7

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000174840
CleanExiHS_PDE12
ExpressionAtlasiQ6L8Q7 baseline and differential
GenevisibleiQ6L8Q7 HS

Organism-specific databases

HPAiHPA043171

Interactioni

Protein-protein interaction databases

BioGridi128397, 30 interactors
IntActiQ6L8Q7, 9 interactors
STRINGi9606.ENSP00000309142

Structurei

Secondary structure

1609
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi163 – 168Combined sources6
Beta strandi182 – 187Combined sources6
Helixi190 – 192Combined sources3
Beta strandi193 – 202Combined sources10
Helixi214 – 216Combined sources3
Beta strandi223 – 227Combined sources5
Beta strandi232 – 235Combined sources4
Helixi239 – 241Combined sources3
Beta strandi246 – 251Combined sources6
Beta strandi262 – 265Combined sources4
Helixi279 – 282Combined sources4
Helixi283 – 285Combined sources3
Beta strandi294 – 301Combined sources8
Helixi305 – 308Combined sources4
Helixi311 – 315Combined sources5
Helixi323 – 325Combined sources3
Helixi328 – 341Combined sources14
Beta strandi345 – 353Combined sources9
Helixi354 – 359Combined sources6
Helixi361 – 367Combined sources7
Beta strandi370 – 387Combined sources18
Turni388 – 390Combined sources3
Beta strandi391 – 396Combined sources6
Helixi401 – 407Combined sources7
Helixi409 – 411Combined sources3
Helixi412 – 417Combined sources6
Turni418 – 420Combined sources3
Helixi422 – 430Combined sources9
Beta strandi434 – 444Combined sources11
Beta strandi449 – 456Combined sources8
Helixi464 – 483Combined sources20
Beta strandi486 – 488Combined sources3
Beta strandi491 – 496Combined sources6
Helixi504 – 511Combined sources8
Beta strandi512 – 514Combined sources3
Helixi519 – 522Combined sources4
Beta strandi540 – 542Combined sources3
Beta strandi550 – 553Combined sources4
Beta strandi556 – 558Combined sources3
Beta strandi561 – 566Combined sources6
Turni567 – 569Combined sources3
Beta strandi570 – 575Combined sources6
Helixi581 – 585Combined sources5
Beta strandi588 – 592Combined sources5
Beta strandi595 – 599Combined sources5
Beta strandi602 – 608Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z0VX-ray1.78A155-609[»]
4Z2BX-ray1.80A155-609[»]
4ZKFX-ray1.82A154-609[»]
ProteinModelPortaliQ6L8Q7
SMRiQ6L8Q7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000006935
HOVERGENiHBG061027
KOiK19612
OMAiDSSKRIC
OrthoDBiEOG091G097K
PhylomeDBiQ6L8Q7
TreeFamiTF323175

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6L8Q7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRLPGARAA LRVIRTAVEK LSRAEAGSQT AAGAMERAVV RCVPSEPKLS
60 70 80 90 100
LSFALADGSH KNMQRDQSEP LGRVLSRIAT NALKGHAKAA AAKKSRKSRP
110 120 130 140 150
NASGGAACSG PGPEPAVFCE PVVKLYYREE AVAEDVLNVD AWQDGAVLQI
160 170 180 190 200
GDVKYKVERN PPAFTELQLP RYIMAGFPVC PKLSLEFGDP ASSLFRWYKE
210 220 230 240 250
AKPGAAEPEV GVPSSLSPSS PSSSWTETDV EERVYTPSNA DIGLRLKLHC
260 270 280 290 300
TPGDGQRFGH SRELESVCVV EAGPGTCTFD HRHLYTKKVT EDALIRTVSY
310 320 330 340 350
NILADTYAQT EFSRTVLYPY CAPYALELDY RQNLIQKELT GYNADVICLQ
360 370 380 390 400
EVDRAVFSDS LVPALEAFGL EGVFRIKQHE GLATFYRKSK FSLLSQHDIS
410 420 430 440 450
FYEALESDPL HKELLEKLVL YPSAQEKVLQ RSSVLQVSVL QSTKDSSKRI
460 470 480 490 500
CVANTHLYWH PKGGYIRLIQ MAVALAHIRH VSCDLYPGIP VIFCGDFNST
510 520 530 540 550
PSTGMYHFVI NGSIPEDHED WASNGEEERC NMSLTHFFKL KSACGEPAYT
560 570 580 590 600
NYVGGFHGCL DYIFIDLNAL EVEQVIPLPS HEEVTTHQAL PSVSHPSDHI

ALVCDLKWK
Length:609
Mass (Da):67,352
Last modified:March 18, 2008 - v2
Checksum:i1D1F3AB4795B52A4
GO
Isoform 2 (identifier: Q6L8Q7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     527-535: EERCNMSLT → GGFGGNFLL
     536-609: Missing.

Show »
Length:535
Mass (Da):58,890
Checksum:i900326406AB885B2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03969823R → W2 PublicationsCorresponds to variant dbSNP:rs2241988Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032202527 – 535EERCNMSLT → GGFGGNFLL in isoform 2. 1 Publication9
Alternative sequenceiVSP_032203536 – 609Missing in isoform 2. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB115695 mRNA Translation: BAD20938.1
AK074423 mRNA Translation: BAB85079.1
AK300374 mRNA Translation: BAG62110.1
CH471055 Genomic DNA Translation: EAW65342.1
AL831824 mRNA Translation: CAD38538.1
CCDSiCCDS33772.1 [Q6L8Q7-1]
RefSeqiNP_808881.3, NM_177966.6 [Q6L8Q7-1]
UniGeneiHs.572993

Genome annotation databases

EnsembliENST00000311180; ENSP00000309142; ENSG00000174840 [Q6L8Q7-1]
GeneIDi201626
KEGGihsa:201626
UCSCiuc003diw.5 human [Q6L8Q7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPDE12_HUMAN
AccessioniPrimary (citable) accession number: Q6L8Q7
Secondary accession number(s): B4DTU8
, Q8IYU3, Q8NDU2, Q8TE78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 25, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health