Q6L8F0 (Q6L8F0_THETH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Medium-chain-fatty-acid--CoA ligase EMBL BAD20228.1
Organism	Thermus thermophilus EMBL BAD20228.1
Taxonomic identifier	274 [NCBI]
Taxonomic lineage	Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Nucleotide-binding PDB 1V26
Molecular function	Ligase EMBL BAD20228.1
Technical term	3D-structure PDB 1V26 PDB 1ULT PDB 1V25
Gene Ontology (GO)
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	302 – 303	2	AMP PDB 1V26
Sites
	Binding site	323	1	AMP; via carbonyl oxygen PDB 1V26
	Binding site	327	1	AMP PDB 1V26
	Binding site	418	1	AMP PDB 1V26
	Binding site	435	1	AMP PDB 1V26
	Binding site	439	1	AMP PDB 1V26

Sequences

	Sequence		Length	Mass (Da)
	Q6L8F0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5243A09836304D42	FASTA	541	59,571
	10 20 30 40 50 60 MEGERMNAFP STMMDEELNL WDFLERAAAL FGRKEVVSRL HTGEVHRTTY AEVYQRARRL 70 80 90 100 110 120 MGGLRALGVG VGDRVATLGF NHFRHLEAYF AVPGMGAVLH TANPRLSPKE IAYILNHAED 130 140 150 160 170 180 KVLLFDPNLL PLVEAIRGEL KTVQHFVVMD EKAPEGYLAY EEALGEEADP VRVPERAACG 190 200 210 220 230 240 MAYTTGTTGL PKGVVYSHRA LVLHSLAASL VDGTALSEKD VVLPVVPMFH VNAWCLPYAA 250 260 270 280 290 300 TLVGAKQVLP GPRLDPASLV ELFDGEGVTF TAGVPTVWLA LADYLESTGH RLKTLRRLVV 310 320 330 340 350 360 GGSAAPRSLI ARFERMGVEV RQGYGLTETS PVVVQNFVKS HLESLSEEEK LTLKAKTGLP 370 380 390 400 410 420 IPLVRLRVAD EEGRPVPKDG KALGEVQLKG PWITGGYYGN EEATRSALTP DGFFRTGDIA 430 440 450 460 470 480 VWDEEGYVEI KDRLKDLIKS GGEWISSVDL ENALMGHPKV KEAAVVAIPH PKWQERPLAV 490 500 510 520 530 540 VVPRGEKPTP EELNEHLLKA GFAKWQLPDA YVFAEEIPRT SAGKFLKRAL REQYKNYYGG A

References

[1]	"medium-chain-fatty-acid--CoA ligase." Miyano M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: HB8 EMBL BAD20228.1.
[2]	Masui R., Inoue Y., Shibata T., Miki K., Yokoyama S., Kuramitsu S. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: HB8 EMBL BAD20228.1.
[3]	"Structural basis of the substrate-specific two-step catalysis of long chain fatty acyl-CoA synthetase dimer." Hisanaga Y., Ago H., Nakagawa N., Hamada K., Ida K., Yamamoto M., Hori T., Arii Y., Sugahara M., Kuramitsu S., Yokoyama S., Miyano M. J. Biol. Chem. 279:31717-31726(2004) [PubMed: 15145952] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH AMP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB126656 Genomic DNA. Translation: BAD20228.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ULT	X-ray	2.55	A/B	1-541	[»]
1V25	X-ray	2.30	A/B	1-541	[»]
1V26	X-ray	2.50	A/B	1-541	[»]

ProteinModelPortal

Q6L8F0.

SMR

Q6L8F0. Positions 8-540.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]

Pfam

PF00501. AMP-binding. 1 hit.
[Graphical view]

PROSITE

PS00455. AMP_BINDING. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q6L8F0_THETH

Accession

Primary (citable) accession number: Q6L8F0

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	July 27, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)