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Protein

Protein phosphatase 2C 50

Gene

PP2C50

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase involved in abscisic acid (ABA) signaling. Together with PYL3 and SAPK10, may form an ABA signaling module involved in stress response.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi118Manganese 1By similarity1
Metal bindingi118Manganese 2By similarity1
Metal bindingi119Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi306Manganese 2By similarity1
Metal bindingi368Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processAbscisic acid signaling pathway
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 50Curated (EC:3.1.3.161 Publication)
Short name:
OsPP2C501 Publication
Alternative name(s):
ABI1-like protein 3Curated
Short name:
OsABI-LIKE31 Publication
Short name:
OsABIL31 Publication
Gene namesi
Name:PP2C501 Publication
Synonyms:ABIL31 Publication
Ordered Locus Names:Os05g0537400Imported, LOC_Os05g46040Curated
ORF Names:OJ1741_B01.18Imported, OSJNBa0052K01.2Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 5

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265S → F: Decreases binding affinity to PYL3 15-fold; when associated with M-267. 1 Publication1
Mutagenesisi265S → K: Abolishes interaction with PYR/PYL/RCAR abscisic acid intracellular receptors; when associated with K-267. 1 Publication1
Mutagenesisi267I → K: Abolishes interaction with PYR/PYL/RCAR abscisic acid intracellular receptors; when associated with K-265. 1 Publication1
Mutagenesisi267I → M: Decreases binding affinity to PYL3 15-fold; when associated with F-265. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003632971 – 387Protein phosphatase 2C 50Add BLAST387

Proteomic databases

PaxDbiQ6L5H6

Expressioni

Inductioni

Induced by abscisic acid (ABA).1 Publication

Interactioni

Subunit structurei

Interacts with PYL3, PYL5, PYL9 and PYL10. Binding to PYL3, PYL5, PYL9 and PYL10 is dependent on the presence of abscisic acid (ABA). Interacts with SAPK10.1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os05g46040.1

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 66Combined sources7
Beta strandi69 – 72Combined sources4
Beta strandi75 – 87Combined sources13
Helixi88 – 90Combined sources3
Helixi94 – 99Combined sources6
Turni104 – 106Combined sources3
Beta strandi108 – 121Combined sources14
Helixi124 – 148Combined sources25
Turni149 – 151Combined sources3
Helixi156 – 175Combined sources20
Beta strandi203 – 208Combined sources6
Beta strandi210 – 220Combined sources11
Beta strandi222 – 227Combined sources6
Beta strandi230 – 234Combined sources5
Helixi243 – 251Combined sources9
Beta strandi256 – 264Combined sources9
Turni265 – 267Combined sources3
Helixi277 – 279Combined sources3
Turni280 – 282Combined sources3
Beta strandi288 – 293Combined sources6
Beta strandi298 – 304Combined sources7
Helixi306 – 309Combined sources4
Helixi314 – 331Combined sources18
Helixi348 – 364Combined sources17
Beta strandi370 – 376Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GWOX-ray2.82A/B59-385[»]
5GWPX-ray2.58A/B59-385[»]
ProteinModelPortaliQ6L5H6
SMRiQ6L5H6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 377PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST318

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi264 – 268Modulates binding affinity to PYR/PYL/RCAR abscisic acid intracellular receptors1 Publication5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 9Poly-Ala8

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
HOGENOMiHOG000233896
InParanoidiQ6L5H6
KOiK14497
OMAiHIDPCAR
OrthoDBiEOG09360DDO

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6L5H6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAAAAI CGEDETAARV GCTGEWAGGI ERVDLGERKE AVAAAGAGKR
60 70 80 90 100
SVYLMDCAPV WGCASTRGRS AEMEDASAAV PRFADVPVRL LASRRDLDAL
110 120 130 140 150
GLDADALRLP AHLFGVFDGH GGAEVANYCR ERIHVVLSEE LKRLGKNLGE
160 170 180 190 200
MGEVDMKEHW DDVFTKCFQR VDDEVSGRVT RVVNGGGEVR SEPVTAENVG
210 220 230 240 250
STAVVALVCS SHVVVANCGD SRIVLCRGKE PVALSIDHKP DRKDERARIE
260 270 280 290 300
AQGGKVIQWN GYRVSGILAM SRSIGDRYLK PFVIPKPEVM VVPRAKDDDC
310 320 330 340 350
LILASDGLWD VVSNEEACKV ARRQILLWHK NNGAASPLSD EGEGSTDPAA
360 370 380
QAAADYLMRL ALKKGSEDNI TVIVVDLKPR KKLKNIS
Length:387
Mass (Da):41,554
Last modified:July 5, 2004 - v1
Checksum:i8DE5BAF0E1CCA2E6
GO
Isoform 2 (identifier: Q6L5H6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-296: DRYLKPFVIPKPEVMVVPRAK → MHIDPCARSRLKHLSVSFPCK
     297-387: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:296
Mass (Da):31,638
Checksum:i2857CCC23E6DE330
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036271276 – 296DRYLK…VPRAK → MHIDPCARSRLKHLSVSFPC K in isoform 2. CuratedAdd BLAST21
Alternative sequenceiVSP_036272297 – 387Missing in isoform 2. CuratedAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC097112 Genomic DNA Translation: AAT39223.1
AC119291 Genomic DNA Translation: AAV59393.1
AP008211 Genomic DNA Translation: BAF18083.2
AP014961 Genomic DNA Translation: BAS95091.1
RefSeqiXP_015639502.1, XM_015784016.1 [Q6L5H6-1]

Genome annotation databases

EnsemblPlantsiOs05t0537400-00; Os05t0537400-00; Os05g0537400 [Q6L5H6-2]
GeneIDi4339454
GrameneiOs05t0537400-00; Os05t0537400-00; Os05g0537400 [Q6L5H6-2]
KEGGiosa:4339454

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiP2C50_ORYSJ
AccessioniPrimary (citable) accession number: Q6L5H6
Secondary accession number(s): Q0DGE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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