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Q6L534

- LISC2_ORYSJ

UniProt

Q6L534 - LISC2_ORYSJ

Protein

Lipoyl synthase 2, chloroplastic

Gene

LIP1P-2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase 2, chloroplastic (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthase 2UniRule annotation
    Short name:
    LS 2UniRule annotation
    Short name:
    Lip-syn 2UniRule annotation
    Lipoate synthase, plastidial 2UniRule annotation
    Short name:
    LIP1p 2UniRule annotation
    Lipoic acid synthase 2UniRule annotation
    Gene namesi
    Name:LIP1P-2UniRule annotation
    Ordered Locus Names:Os05g0511500, LOC_Os05g43576
    ORF Names:B1155G07.1, OJ1005_B11.11, OsJ_19166
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 5

    Organism-specific databases

    GrameneiQ6L534.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848ChloroplastUniRule annotationAdd
    BLAST
    Chaini49 – 384336Lipoyl synthase 2, chloroplasticPRO_0000398864Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ6L534.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiHIATIGQ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6L534-1 [UniParc]FASTAAdd to Basket

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    MAAYCSRVYH HHPVSPSTMQ GSLARPSIHA GSASLTFRAR PNSVSIVRCD    50
    ADSPPEGSAV AGWAPPGPYT GRDPAARKPA WLRQRAAQGE KYARLRESLG 100
    ELKLNTVCVE AQCPNIGECW DGGGGAGGDG DGIATATIML LGDTCTRGCR 150
    FCAVKTSNKP PPPDALEPLR TAVAVASWGV DYVVLTSVDR DDLPDGGSGH 200
    FAQTVKALKE LKPGILVECL TSDFRGDLEA VSSLASSGLD VFAHNIETVR 250
    SLQRIVRDPR AAYDQSLAVL KHAKNCKDGM VTKSSIMLGL GETDEEVKQT 300
    MCDLRAIDVD ILTLGQYLQP TERHLRVREY VTPEKFDFWK EYGESLGFLY 350
    VASGPLVRSS YRAGELFVQN LVRRKKAELA PTLQ 384
    Length:384
    Mass (Da):41,546
    Last modified:July 5, 2004 - v1
    Checksum:i4F0C3FA049ADFFA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC108873 Genomic DNA. Translation: AAT44142.1.
    AC130604 Genomic DNA. Translation: AAV59287.1.
    AP008211 Genomic DNA. Translation: BAF17930.1.
    CM000142 Genomic DNA. Translation: EEE64327.1.
    AK101807 mRNA. Translation: BAG95240.1.
    RefSeqiNP_001056016.1. NM_001062551.1.
    UniGeneiOs.7615.

    Genome annotation databases

    EnsemblPlantsiOS05T0511500-01; OS05T0511500-01; OS05G0511500.
    GeneIDi4339289.
    KEGGiosa:4339289.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC108873 Genomic DNA. Translation: AAT44142.1 .
    AC130604 Genomic DNA. Translation: AAV59287.1 .
    AP008211 Genomic DNA. Translation: BAF17930.1 .
    CM000142 Genomic DNA. Translation: EEE64327.1 .
    AK101807 mRNA. Translation: BAG95240.1 .
    RefSeqi NP_001056016.1. NM_001062551.1.
    UniGenei Os.7615.

    3D structure databases

    ProteinModelPortali Q6L534.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS05T0511500-01 ; OS05T0511500-01 ; OS05G0511500 .
    GeneIDi 4339289.
    KEGGi osa:4339289.

    Organism-specific databases

    Gramenei Q6L534.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi HIATIGQ.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiLISC2_ORYSJ
    AccessioniPrimary (citable) accession number: Q6L534
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3