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Protein

Lipoyl synthase 2, chloroplastic

Gene

LIP1P-2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase 1, chloroplastic (LIP1P-1), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase 2, chloroplastic (LIP1P-2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi113Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi145Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi149Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi152Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-OSA-389661 Glyoxylate metabolism and glycine degradation
UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 2UniRule annotation
Short name:
LS 2UniRule annotation
Short name:
Lip-syn 2UniRule annotation
Lipoate synthase, plastidial 2UniRule annotation
Short name:
LIP1p 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Gene namesi
Name:LIP1P-2UniRule annotation
Ordered Locus Names:Os05g0511500, LOC_Os05g43576
ORF Names:B1155G07.1, OJ1005_B11.11, OsJ_19166
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastUniRule annotationAdd BLAST48
ChainiPRO_000039886449 – 384Lipoyl synthase 2, chloroplasticAdd BLAST336

Proteomic databases

PaxDbiQ6L534

Expressioni

Gene expression databases

ExpressionAtlasiQ6L534 baseline and differential
GenevisibleiQ6L534 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g43576.1

Structurei

3D structure databases

ProteinModelPortaliQ6L534
SMRiQ6L534
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
InParanoidiQ6L534
KOiK03644
OMAiEAKKPAW
OrthoDBiEOG093604CP

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03129 Lipoyl_synth_plantC, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR027526 Lipoyl_synth_chlpt
IPR007197 rSAM
PANTHERiPTHR10949:SF14 PTHR10949:SF14, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6L534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAYCSRVYH HHPVSPSTMQ GSLARPSIHA GSASLTFRAR PNSVSIVRCD
60 70 80 90 100
ADSPPEGSAV AGWAPPGPYT GRDPAARKPA WLRQRAAQGE KYARLRESLG
110 120 130 140 150
ELKLNTVCVE AQCPNIGECW DGGGGAGGDG DGIATATIML LGDTCTRGCR
160 170 180 190 200
FCAVKTSNKP PPPDALEPLR TAVAVASWGV DYVVLTSVDR DDLPDGGSGH
210 220 230 240 250
FAQTVKALKE LKPGILVECL TSDFRGDLEA VSSLASSGLD VFAHNIETVR
260 270 280 290 300
SLQRIVRDPR AAYDQSLAVL KHAKNCKDGM VTKSSIMLGL GETDEEVKQT
310 320 330 340 350
MCDLRAIDVD ILTLGQYLQP TERHLRVREY VTPEKFDFWK EYGESLGFLY
360 370 380
VASGPLVRSS YRAGELFVQN LVRRKKAELA PTLQ
Length:384
Mass (Da):41,546
Last modified:July 5, 2004 - v1
Checksum:i4F0C3FA049ADFFA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC108873 Genomic DNA Translation: AAT44142.1
AC130604 Genomic DNA Translation: AAV59287.1
AP008211 Genomic DNA Translation: BAF17930.1
AP014961 Genomic DNA Translation: BAS94844.1
CM000142 Genomic DNA Translation: EEE64327.1
AK101807 mRNA Translation: BAG95240.1
RefSeqiXP_015638121.1, XM_015782635.1
UniGeneiOs.7615

Genome annotation databases

EnsemblPlantsiOs05t0511500-01; Os05t0511500-01; Os05g0511500
GeneIDi4339289
GrameneiOs05t0511500-01; Os05t0511500-01; Os05g0511500
KEGGiosa:4339289

Similar proteinsi

Entry informationi

Entry nameiLISC2_ORYSJ
AccessioniPrimary (citable) accession number: Q6L534
Secondary accession number(s): A0A0P0WPH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome