Q6L4I2 (GUN15_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 15
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 15
OsCel9C

Gene names

Ordered Locus Names:	Os05g0212300, LOC_Os05g12150
ORF Names:	OSJNBa0074P11.11

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 629	599	Endoglucanase 15		PRO_0000249292
Sites
	Active site	421	1	By similarity
	Active site	473	1	By similarity
	Active site	482	1	By similarity
Amino acid modifications
	Glycosylation	520	1	N-linked (GlcNAc...) Potential
	Glycosylation	540	1	N-linked (GlcNAc...) Potential
	Glycosylation	561	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	284	1	G → E in AK063458. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6L4I2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 11FBA6DCF13F66DE	FASTA	629	68,664
	10 20 30 40 50 60 MAKNGGAHGA ATLFGLLALA SMVKLGFVAG GGHDYAMALR KSILYFQAQR SGVLPPNQRV 70 80 90 100 110 120 SWRASSGLFD GKANGVDLVG GYYDAGDNVK FGLPMAFTVT MMSWSILEYG KQMAAAGELR 130 140 150 160 170 180 NAMDAVKWGT DYFIKAHPEP DVLYGEVGDG DTDHSCWQRP EDMTTSRQAF RVDPQHPGSD 190 200 210 220 230 240 LAAETAAAMA AASIVFRGTY PGYANLLLVH SKQLFEFADK YRGKYDASIT VARNYYGSFS 250 260 270 280 290 300 GYGDELLWAA AWLFEATEDR SYLEYLAGNG EALGGTGWSI NQFGWDVKYP GVQVLAAKFL 310 320 330 340 350 360 LQGRAGDHAA ALQRYRQNAE FFVCSCVGKG AVNVARTPGG MMYHQRWNNL QFVTSASFLL 370 380 390 400 410 420 TVYADFAAIS GRGAVHCPAG AAQPFDILKF VKSQVNYILG DNPRGTSYMV GYGASYPRQV 430 440 450 460 470 480 HHRGASIVSI KRDPSFVSCQ EGYSSWYGRE AGNPNLLDGA VVGGPDEYDD FADERDNYEQ 490 500 510 520 530 540 TEAATYNNAP LLGVLARLAA SCGGLKEEEY EQETATPVVN RTSSSSSLPA TATAIGIEQN 550 560 570 580 590 600 VTGTWARRRR TYYRYAVTVT NRSRGKTVRE LHLGVSGLRG RLWGLEEARY GYVPPRWLPA 610 620 LRPGRSLRFV YVQPAPAPAN IWVTGYKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC135914 Genomic DNA. Translation: AAT44235.1. AP008211 Genomic DNA. Translation: BAF16834.1. AK063458 mRNA. No translation available.
RefSeq	NP_001054920.1. NM_001061455.1.
UniGene	Os.51700.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q6L4I2.
ModBase	Search...
Protein family/group databases
CAZy	CBM49. Carbohydrate-Binding Module Family 49. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g12150.1; LOC_Os05g12150.1; LOC_Os05g12150.
GeneID	4338100.
KEGG	osa:4338100.
Organism-specific databases
Gramene	Q6L4I2.
Phylogenomic databases
eggNOG	NOG05134.
OMA	PAPANIW.
ProtClustDB	PLN02171.
Gene expression databases
ArrayExpress	Q6L4I2.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR019028. CBM_49. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF09478. CBM49. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN15_ORYSJ

Accession

Primary (citable) accession number: Q6L4I2
Secondary accession number(s): Q0DJY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program