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Protein

Probable protein phosphatase 2C 48

Gene

Os05g0358500

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Manganese 1By similarity1
Metal bindingi90Manganese 2By similarity1
Metal bindingi91Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi293Manganese 2By similarity1
Metal bindingi339Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-OSA-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 48 (EC:3.1.3.16)
Short name:
OsPP2C48
Gene namesi
Ordered Locus Names:Os05g0358500, LOC_Os05g29030
ORF Names:OJ1045_C06.13, OSJNBa0009L15.3
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003632951 – 353Probable protein phosphatase 2C 48Add BLAST353

Proteomic databases

PaxDbiQ6L482.
PRIDEiQ6L482.

Expressioni

Gene expression databases

GenevisibleiQ6L482. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g29030.1.

Structurei

3D structure databases

ProteinModelPortaliQ6L482.
SMRiQ6L482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 348PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST295

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 38Poly-Ala8

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233897.
InParanoidiQ6L482.
OMAiCDAKEAA.
OrthoDBiEOG09360GNF.

Family and domain databases

CDDicd00143. PP2Cc. 1 hit.
Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR036457. PPM-type_dom_sf.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiView protein in Pfam
PF00481. PP2C. 1 hit.
SMARTiView protein in SMART
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiView protein in PROSITE
PS51746. PPM_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6L482-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHISSLLQG LARSLSVGKE RKGGDGDDGK AAAATATAVL RTSGTLWGEG
60 70 80 90 100
SETFAAVCSR RGEKGINQDC SIVCEGFGCE EGSVLCGIFD GHGQWGHYVA
110 120 130 140 150
KAVRESLPPA LLRRWREAVT LAALIDGGEK RLCECRPDLW RQSYLAACAA
160 170 180 190 200
VDAELRASRR LDAVHSGCTA LSLVKHGDLL VVANVGDSRA VLATASPDDG
210 220 230 240 250
GGARLAAVQL TVDFKPNLPQ ERERIMECNG RVQCLADEPG VHRVWRPDRE
260 270 280 290 300
GPGLAMSRAF GDYCVKDYGV ISAPEVTHRR ITAQDHFVIL ATDGVWDVVS
310 320 330 340 350
NEEAVQIVAS APEREKAAKR LVEFAVRAWR RKRRGIAVDD CSAICLFFHS

PPS
Length:353
Mass (Da):38,101
Last modified:July 5, 2004 - v1
Checksum:i08013ADEAE50D4C2
GO
Isoform 2 (identifier: Q6L482-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     295-353: VWDVVSNEEA...ICLFFHSPPS → DKHLNLFVFV...TRRRQCRRRR

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:391
Mass (Da):41,972
Checksum:i8FAA97D0189CE654
GO

Sequence cautioni

The sequence AK100777 differs from that shown. Reason: Frameshift at position 277.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036270295 – 353VWDVV…HSPPS → DKHLNLFVFVCAAGVGRGVE RGGGADRGVGAGEGEGGEAA RRVRRPGMEAQAPGHRRRRL LGDLPLLPLAAVLNNTHADT HAANKNRTRRRQCRRRR in isoform 2. CuratedAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC104272 Genomic DNA. Translation: AAV43855.1.
AC136999 Genomic DNA. Translation: AAT38039.1.
AP008211 Genomic DNA. Translation: BAF17217.1.
AP014961 Genomic DNA. Translation: BAS93593.1.
AK100777 mRNA. No translation available.
RefSeqiXP_015639910.1. XM_015784424.1. [Q6L482-2]
XP_015639911.1. XM_015784425.1. [Q6L482-1]
UniGeneiOs.9646.

Genome annotation databases

EnsemblPlantsiOS05T0358500-01; OS05T0358500-01; OS05G0358500. [Q6L482-1]
OS05T0358500-02; OS05T0358500-02; OS05G0358500. [Q6L482-1]
GeneIDi4338531.
GrameneiOS05T0358500-01; OS05T0358500-01; OS05G0358500.
OS05T0358500-02; OS05T0358500-02; OS05G0358500.
KEGGiosa:4338531.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiP2C48_ORYSJ
AccessioniPrimary (citable) accession number: Q6L482
Secondary accession number(s): A0A0N7KKM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 5, 2004
Last modified: October 25, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families