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Q6L243

- KAE1B_PICTO

UniProt

Q6L243 - KAE1B_PICTO

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Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
PTO0374
Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron By similarity
Metal bindingi111 – 1111Iron By similarity
Metal bindingi128 – 1281Iron By similarity
Binding sitei160 – 1601Threonylcarbamoyl-AMP By similarity
Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei177 – 1771Threonylcarbamoyl-AMP By similarity
Binding sitei257 – 2571Threonylcarbamoyl-AMP By similarity
Metal bindingi285 – 2851Iron By similarity
Binding sitei355 – 3551ATP By similarity
Active sitei447 – 4471Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi335 – 3428ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  6. protein tyrosine kinase activity Source: InterPro
  7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. lipopolysaccharide biosynthetic process Source: InterPro
  2. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:PTO0374
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
ProteomesiUP000000438: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
PRO_0000303660Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi263820.PTO0374.

Structurei

3D structure databases

ProteinModelPortaliQ6L243.
SMRiQ6L243. Positions 1-325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 529199Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324Kae1UniRule annotation
Add
BLAST
Regioni128 – 1325Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6L243-1 [UniParc]FASTAAdd to Basket

« Hide

MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK    50
IYDVIKRSFD NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK 100
PLLGVNHPLG HVEIGRKLSG ARDPIMLYIS GGNTQVIAHL NGRYRVLGET 150
MDIGLGNMLD KFARDLGIPF PGGPVIERMA LDGKDLLELP YSVKGMDTSF 200
SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM YYTNKNEILL 250
AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH 300
GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR 350
SVVFKKRPEK SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR 400
FDMVLTIERI NGLTMNDYFK SKYDENVIRM IADAVAKMHG LRISHGDLTP 450
NNIIISDKIY FIDTSMGHLR CSDEDIADDL FLLYESMKSL YVNGHEMIKH 500
FENEYSKNVE DYNYYKELIK KIESRRRYV 529
Length:529
Mass (Da):60,103
Last modified:July 5, 2004 - v1
Checksum:i230A4D3F0DA22B02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeqiWP_011177175.1. NC_005877.1.
YP_023152.1. NC_005877.1.

Genome annotation databases

EnsemblBacteriaiAAT42959; AAT42959; PTO0374.
GeneIDi2845198.
KEGGipto:PTO0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1 .
RefSeqi WP_011177175.1. NC_005877.1.
YP_023152.1. NC_005877.1.

3D structure databases

ProteinModelPortali Q6L243.
SMRi Q6L243. Positions 1-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 263820.PTO0374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT42959 ; AAT42959 ; PTO0374 .
GeneIDi 2845198.
KEGGi pto:PTO0374.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci PTOR263820:GHA3-388-MONOMER.

Family and domain databases

HAMAPi MF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Entry informationi

Entry nameiKAE1B_PICTO
AccessioniPrimary (citable) accession number: Q6L243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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