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Q6L243 (KAE1B_PICTO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.6.99.4
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name=t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:PTO0374
OrganismPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]
Taxonomic identifier263820 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303660

Regions

Domain331 – 529199Protein kinase
Nucleotide binding335 – 3428ATP By similarity
Region1 – 324324Kae1 HAMAP-Rule MF_01447
Region128 – 1325Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4471Proton acceptor; for kinase activity By similarity
Metal binding1071Iron By similarity
Metal binding1111Iron By similarity
Metal binding1281Iron By similarity
Metal binding2851Iron By similarity
Binding site1601Threonylcarbamoyl-AMP By similarity
Binding site1731Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1771Threonylcarbamoyl-AMP By similarity
Binding site2571Threonylcarbamoyl-AMP By similarity
Binding site3551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6L243 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 230A4D3F0DA22B02

FASTA52960,103
        10         20         30         40         50         60 
MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK IYDVIKRSFD 

        70         80         90        100        110        120 
NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK PLLGVNHPLG HVEIGRKLSG 

       130        140        150        160        170        180 
ARDPIMLYIS GGNTQVIAHL NGRYRVLGET MDIGLGNMLD KFARDLGIPF PGGPVIERMA 

       190        200        210        220        230        240 
LDGKDLLELP YSVKGMDTSF SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM 

       250        260        270        280        290        300 
YYTNKNEILL AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH 

       310        320        330        340        350        360 
GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR SVVFKKRPEK 

       370        380        390        400        410        420 
SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR FDMVLTIERI NGLTMNDYFK 

       430        440        450        460        470        480 
SKYDENVIRM IADAVAKMHG LRISHGDLTP NNIIISDKIY FIDTSMGHLR CSDEDIADDL 

       490        500        510        520 
FLLYESMKSL YVNGHEMIKH FENEYSKNVE DYNYYKELIK KIESRRRYV 

« Hide

References

[1]"Genome sequence of Picrophilus torridus and its implications for life around pH 0."
Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W.
Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeqYP_023152.1. NC_005877.1.

3D structure databases

ProteinModelPortalQ6L243.
SMRQ6L243. Positions 1-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING263820.PTO0374.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT42959; AAT42959; PTO0374.
GeneID2845198.
KEGGpto:PTO0374.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.

Enzyme and pathway databases

BioCycPTOR263820:GHA3-388-MONOMER.

Family and domain databases

HAMAPMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_PICTO
AccessionPrimary (citable) accession number: Q6L243
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families