Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein - Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)

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Q6L243 (KAE1B_PICTO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
Probable serine/threonine-protein kinase BUD32 homolog
EC=2.7.11.1

Gene names

Ordered Locus Names:

PTO0374

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus ›

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t₆A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP-Rule MF_01447
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01447.
Sequence similarities	In the N-terminal section; belongs to the KAE1 / YgjD family. In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Iron Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 529

529

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447

PRO_0000303660

Regions

Domain

331 – 529

199

Protein kinase

Nucleotide binding

335 – 342

ATP By similarity

Region

1 – 324

324

Kae1 HAMAP-Rule MF_01447

Sites

Active site

447

Proton acceptor By similarity

Metal binding

107

Iron By similarity

Metal binding

111

Iron By similarity

Metal binding

128

Iron By similarity

Metal binding

285

Iron By similarity

Binding site

355

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6L243 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 230A4D3F0DA22B02
Show »

FASTA

529

60,103

        10         20         30         40         50         60 
MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK IYDVIKRSFD 

        70         80         90        100        110        120 
NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK PLLGVNHPLG HVEIGRKLSG 

       130        140        150        160        170        180 
ARDPIMLYIS GGNTQVIAHL NGRYRVLGET MDIGLGNMLD KFARDLGIPF PGGPVIERMA 

       190        200        210        220        230        240 
LDGKDLLELP YSVKGMDTSF SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM 

       250        260        270        280        290        300 
YYTNKNEILL AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH 

       310        320        330        340        350        360 
GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR SVVFKKRPEK 

       370        380        390        400        410        420 
SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR FDMVLTIERI NGLTMNDYFK 

       430        440        450        460        470        480 
SKYDENVIRM IADAVAKMHG LRISHGDLTP NNIIISDKIY FIDTSMGHLR CSDEDIADDL 

       490        500        510        520 
FLLYESMKSL YVNGHEMIKH FENEYSKNVE DYNYYKELIK KIESRRRYV

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References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0."
Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W.
Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeq	YP_023152.1. NC_005877.1.
3D structure databases
ProteinModelPortal	Q6L243.
SMR	Q6L243. Positions 1-325.
ModBase	Search...
Protein-protein interaction databases
STRING	263820.PTO0374.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT42959; AAT42959; PTO0374.
GeneID	2845198.
KEGG	pto:PTO0374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0533.
HOGENOM	HOG000109569.
KO	K15904.
OMA	GNCLDQF.
ProtClustDB	PRK09605.
Enzyme and pathway databases
BioCyc	PTOR263820:GHA3-375-MONOMER.
Family and domain databases
HAMAP	MF_01447. Kae1_Bud32_arch.
InterPro	IPR000905. Gcp-like_dom. IPR022495. Kae1-assoc_kinase_Bud32. IPR017861. KAE1/YgjD. IPR022449. Kae1_arc. IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR009220. tRNA_threonyl_synthase/kinase. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF06293. Kdo. 1 hit. PF00814. Peptidase_M22. 1 hit. [Graphical view]
PIRSF	PIRSF036401. Gcp_STYKS. 1 hit.
PRINTS	PR00789. OSIALOPTASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. arch_bud32. 1 hit. TIGR03722. arch_KAE1. 1 hit. TIGR00329. gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. False negative. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAE1B_PICTO

Accession

Primary (citable) accession number: Q6L243

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents