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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

PTO0374

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107IronUniRule annotation1
Metal bindingi111IronUniRule annotation1
Metal bindingi128IronUniRule annotation1
Binding sitei160Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei173Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei177Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei257Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi285IronUniRule annotation1
Binding sitei355ATPUniRule annotation1
Active sitei447Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:PTO0374
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
Proteomesi
  • UP000000438 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036601 – 529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST529

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi263820.PTO0374.

Structurei

3D structure databases

ProteinModelPortaliQ6L243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini331 – 529Protein kinaseUniRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 324Kae1Add BLAST324
Regioni128 – 132Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
HOGENOMiHOG000109569.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6L243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK
60 70 80 90 100
IYDVIKRSFD NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK
110 120 130 140 150
PLLGVNHPLG HVEIGRKLSG ARDPIMLYIS GGNTQVIAHL NGRYRVLGET
160 170 180 190 200
MDIGLGNMLD KFARDLGIPF PGGPVIERMA LDGKDLLELP YSVKGMDTSF
210 220 230 240 250
SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM YYTNKNEILL
260 270 280 290 300
AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH
310 320 330 340 350
GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR
360 370 380 390 400
SVVFKKRPEK SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR
410 420 430 440 450
FDMVLTIERI NGLTMNDYFK SKYDENVIRM IADAVAKMHG LRISHGDLTP
460 470 480 490 500
NNIIISDKIY FIDTSMGHLR CSDEDIADDL FLLYESMKSL YVNGHEMIKH
510 520
FENEYSKNVE DYNYYKELIK KIESRRRYV
Length:529
Mass (Da):60,103
Last modified:July 5, 2004 - v1
Checksum:i230A4D3F0DA22B02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeqiWP_011177175.1. NC_005877.1.

Genome annotation databases

EnsemblBacteriaiAAT42959; AAT42959; PTO0374.
GeneIDi2845198.
KEGGipto:PTO0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeqiWP_011177175.1. NC_005877.1.

3D structure databases

ProteinModelPortaliQ6L243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi263820.PTO0374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT42959; AAT42959; PTO0374.
GeneIDi2845198.
KEGGipto:PTO0374.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
HOGENOMiHOG000109569.
KOiK15904.
OMAiGETMDTG.

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-388-MONOMER.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAE1B_PICTO
AccessioniPrimary (citable) accession number: Q6L243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: October 5, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.