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Q6L243

- KAE1B_PICTO

UniProt

Q6L243 - KAE1B_PICTO

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

PTO0374

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi107 – 1071IronUniRule annotation
    Metal bindingi111 – 1111IronUniRule annotation
    Metal bindingi128 – 1281IronUniRule annotation
    Binding sitei160 – 1601Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei177 – 1771Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei257 – 2571Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi285 – 2851IronUniRule annotation
    Binding sitei355 – 3551ATPUniRule annotation
    Active sitei447 – 4471Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi335 – 3428ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. protein tyrosine kinase activity Source: InterPro
    7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. lipopolysaccharide biosynthetic process Source: InterPro
    2. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPTOR263820:GHA3-388-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:PTO0374
    OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
    Taxonomic identifieri263820 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
    ProteomesiUP000000438: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303660Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi263820.PTO0374.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6L243.
    SMRiQ6L243. Positions 1-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini331 – 529199Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 324324Kae1Add
    BLAST
    Regioni128 – 1325Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109569.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01446. Kae1_arch.
    MF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR010440. LipoPS_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF06293. Kdo. 1 hit.
    PF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6L243-1 [UniParc]FASTAAdd to Basket

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    MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK    50
    IYDVIKRSFD NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK 100
    PLLGVNHPLG HVEIGRKLSG ARDPIMLYIS GGNTQVIAHL NGRYRVLGET 150
    MDIGLGNMLD KFARDLGIPF PGGPVIERMA LDGKDLLELP YSVKGMDTSF 200
    SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM YYTNKNEILL 250
    AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH 300
    GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR 350
    SVVFKKRPEK SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR 400
    FDMVLTIERI NGLTMNDYFK SKYDENVIRM IADAVAKMHG LRISHGDLTP 450
    NNIIISDKIY FIDTSMGHLR CSDEDIADDL FLLYESMKSL YVNGHEMIKH 500
    FENEYSKNVE DYNYYKELIK KIESRRRYV 529
    Length:529
    Mass (Da):60,103
    Last modified:July 5, 2004 - v1
    Checksum:i230A4D3F0DA22B02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017261 Genomic DNA. Translation: AAT42959.1.
    RefSeqiWP_011177175.1. NC_005877.1.
    YP_023152.1. NC_005877.1.

    Genome annotation databases

    EnsemblBacteriaiAAT42959; AAT42959; PTO0374.
    GeneIDi2845198.
    KEGGipto:PTO0374.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017261 Genomic DNA. Translation: AAT42959.1 .
    RefSeqi WP_011177175.1. NC_005877.1.
    YP_023152.1. NC_005877.1.

    3D structure databases

    ProteinModelPortali Q6L243.
    SMRi Q6L243. Positions 1-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 263820.PTO0374.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT42959 ; AAT42959 ; PTO0374 .
    GeneIDi 2845198.
    KEGGi pto:PTO0374.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109569.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci PTOR263820:GHA3-388-MONOMER.

    Family and domain databases

    HAMAPi MF_01446. Kae1_arch.
    MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR010440. LipoPS_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF06293. Kdo. 1 hit.
    PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

    Entry informationi

    Entry nameiKAE1B_PICTO
    AccessioniPrimary (citable) accession number: Q6L243
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3