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Q6L243

- KAE1B_PICTO

UniProt

Q6L243 - KAE1B_PICTO

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

PTO0374

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071IronUniRule annotation
Metal bindingi111 – 1111IronUniRule annotation
Metal bindingi128 – 1281IronUniRule annotation
Binding sitei160 – 1601Threonylcarbamoyl-AMPUniRule annotation
Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
Binding sitei177 – 1771Threonylcarbamoyl-AMPUniRule annotation
Binding sitei257 – 2571Threonylcarbamoyl-AMPUniRule annotation
Metal bindingi285 – 2851IronUniRule annotation
Binding sitei355 – 3551ATPUniRule annotation
Active sitei447 – 4471Proton acceptor; for kinase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi335 – 3428ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. lipopolysaccharide biosynthetic process Source: InterPro
  2. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:PTO0374
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
ProteomesiUP000000438: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303660Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi263820.PTO0374.

Structurei

3D structure databases

ProteinModelPortaliQ6L243.
SMRiQ6L243. Positions 1-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 529199Protein kinaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324Kae1Add
BLAST
Regioni128 – 1325Threonylcarbamoyl-AMP bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6L243-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIVLGLEGTA HTISAGIVDE KSILSNVSST YVPEHGGIHP REAAVHHADK
60 70 80 90 100
IYDVIKRSFD NAGLKPEDLD LIAFSMGPGL GPCLRVVSTA ARALSIKYSK
110 120 130 140 150
PLLGVNHPLG HVEIGRKLSG ARDPIMLYIS GGNTQVIAHL NGRYRVLGET
160 170 180 190 200
MDIGLGNMLD KFARDLGIPF PGGPVIERMA LDGKDLLELP YSVKGMDTSF
210 220 230 240 250
SGIYTAAKRY LSLGKNKNDI CYSLQETSFS MVVEVLERAM YYTNKNEILL
260 270 280 290 300
AGGVARNDRL RSMVNDMARD SGYKAYLTDK EYCMDNGAMI AQAGMLMYMH
310 320 330 340 350
GARQDIMETR INQRFRIDEV PAPWIKDENS ISFKDKGAES IITNGEFYGR
360 370 380 390 400
SVVFKKRPEK SYRDKRLDLK IRLERMKNEF YIMYYLHKTG NAPIVYDFDR
410 420 430 440 450
FDMVLTIERI NGLTMNDYFK SKYDENVIRM IADAVAKMHG LRISHGDLTP
460 470 480 490 500
NNIIISDKIY FIDTSMGHLR CSDEDIADDL FLLYESMKSL YVNGHEMIKH
510 520
FENEYSKNVE DYNYYKELIK KIESRRRYV
Length:529
Mass (Da):60,103
Last modified:July 5, 2004 - v1
Checksum:i230A4D3F0DA22B02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1.
RefSeqiWP_011177175.1. NC_005877.1.
YP_023152.1. NC_005877.1.

Genome annotation databases

EnsemblBacteriaiAAT42959; AAT42959; PTO0374.
GeneIDi2845198.
KEGGipto:PTO0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42959.1 .
RefSeqi WP_011177175.1. NC_005877.1.
YP_023152.1. NC_005877.1.

3D structure databases

ProteinModelPortali Q6L243.
SMRi Q6L243. Positions 1-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 263820.PTO0374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT42959 ; AAT42959 ; PTO0374 .
GeneIDi 2845198.
KEGGi pto:PTO0374.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci PTOR263820:GHA3-388-MONOMER.

Family and domain databases

HAMAPi MF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF06293. Kdo. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Entry informationi

Entry nameiKAE1B_PICTO
AccessioniPrimary (citable) accession number: Q6L243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3