Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6L206

- SYI_PICTO

UniProt

Q6L206 - SYI_PICTO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei593 – 5931ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPTOR263820:GHA3-426-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PTO0411
    OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
    Taxonomic identifieri263820 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
    ProteomesiUP000000438: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10241024Isoleucine--tRNA ligasePRO_0000098586Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi263820.PTO0411.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6L206.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi52 – 6211"HIGH" regionAdd
    BLAST
    Motifi590 – 5945"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiNANIDEY.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6L206-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKATLYENIN VNKSMIDINN EILNYWKENH IDENIIKSNR NLKPFAFLEG     50
    PPTANGRPHV GHLMTRAVKD TVMRYKYMTG HDILRRTGGW DCHGLPVELE 100
    AEKHFGFKNK KDIENFGIEK FNQYCRDSVF RYIDEWNIVD DLVGFWVDKE 150
    NSYITLKNDY MESEWWALKT LYENNLLVKD YKIVPYCPRC GTSLSSHEVA 200
    QGYKNVDDPS VFVKFAEKGR KNRYFIAWTT TPWTLPSNEF LVVNPDMDYS 250
    LIESDGFEYY LLSSKVESLF NDYKLIKTFK GRDLEGIEYE QLMPFLEKPE 300
    NAFRVVAASF VTAEDGTGIV HAAPAFGADD FEIGKRFSVE ILNPVDQDGR 350
    FNEKLPWSGL FVTDANKSII NYLKENNLLF KAETMKHDYP FCYRCGTRLL 400
    YYPLDTWFIK VSLIRKKLLE NNEKINWVPD YLKNGRFGNF LEEAKDWALS 450
    RDRYWGTPLP IWRCNKNHYL AIGSRDDLLK YGGYIPEDLH RPYIDDVVLK 500
    CPECGSEMHR ESYVIDTWFD SGSASYAAMH YPFSKDFTKS HFPVDFITEA 550
    IDQTRGWFYT LHVVASLLFD ENAYKNVVSI SHILDENGQK MSKSKGNFIA 600
    AIDFLNDYGA DAARMFFFTG APWNSKSVNK KLIGEITRKN LSTLLNVYSF 650
    FASNANIDEY RFTEIKEPEN LLDRWMLSRL NTTIIKVREN MDNYNIHTAL 700
    RYIEDLISEL SNVYLRLSRK RFWEGNLDDS KERAYSTLYY TLRETIKMMA 750
    PITPFFSEYL YQKLSPGMPS VHMESYPEAI ERFIDNDLEN EMEHAIEIME 800
    LSRRTRQELN IKGRQPVKEI LIYSDIKLRD DIIDIISPEL NAESIRFIKS 850
    DEMPLKITVR ADISKVAKLL KSRINDFNLY LERNNDLVYR ELKSKGKINF 900
    DGIYLTDDMF IMNEEVNGNY GFNKDERSGI YLFINREIDN DLLLEGYARE 950
    IIRRIQVMRK DLNLEYSEKI KTYIDADEDI RSAVERYMEK IKNETLSSEI 1000
    LFKNDPEARA WDIDDKTVYI KIVK 1024
    Length:1,024
    Mass (Da):120,191
    Last modified:July 5, 2004 - v1
    Checksum:i6B23F804B7AAFF9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017261 Genomic DNA. Translation: AAT42996.1.
    RefSeqiWP_011177212.1. NC_005877.1.
    YP_023189.1. NC_005877.1.

    Genome annotation databases

    EnsemblBacteriaiAAT42996; AAT42996; PTO0411.
    GeneIDi2844251.
    KEGGipto:PTO0411.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017261 Genomic DNA. Translation: AAT42996.1 .
    RefSeqi WP_011177212.1. NC_005877.1.
    YP_023189.1. NC_005877.1.

    3D structure databases

    ProteinModelPortali Q6L206.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 263820.PTO0411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT42996 ; AAT42996 ; PTO0411 .
    GeneIDi 2844251.
    KEGGi pto:PTO0411.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi NANIDEY.

    Enzyme and pathway databases

    BioCyci PTOR263820:GHA3-426-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

    Entry informationi

    Entry nameiSYI_PICTO
    AccessioniPrimary (citable) accession number: Q6L206
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3