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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PTO0411
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
ProteomesiUP000000438 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Isoleucine--tRNA ligasePRO_0000098586Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi263820.PTO0411.

Structurei

3D structure databases

ProteinModelPortaliQ6L206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6L206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKATLYENIN VNKSMIDINN EILNYWKENH IDENIIKSNR NLKPFAFLEG
60 70 80 90 100
PPTANGRPHV GHLMTRAVKD TVMRYKYMTG HDILRRTGGW DCHGLPVELE
110 120 130 140 150
AEKHFGFKNK KDIENFGIEK FNQYCRDSVF RYIDEWNIVD DLVGFWVDKE
160 170 180 190 200
NSYITLKNDY MESEWWALKT LYENNLLVKD YKIVPYCPRC GTSLSSHEVA
210 220 230 240 250
QGYKNVDDPS VFVKFAEKGR KNRYFIAWTT TPWTLPSNEF LVVNPDMDYS
260 270 280 290 300
LIESDGFEYY LLSSKVESLF NDYKLIKTFK GRDLEGIEYE QLMPFLEKPE
310 320 330 340 350
NAFRVVAASF VTAEDGTGIV HAAPAFGADD FEIGKRFSVE ILNPVDQDGR
360 370 380 390 400
FNEKLPWSGL FVTDANKSII NYLKENNLLF KAETMKHDYP FCYRCGTRLL
410 420 430 440 450
YYPLDTWFIK VSLIRKKLLE NNEKINWVPD YLKNGRFGNF LEEAKDWALS
460 470 480 490 500
RDRYWGTPLP IWRCNKNHYL AIGSRDDLLK YGGYIPEDLH RPYIDDVVLK
510 520 530 540 550
CPECGSEMHR ESYVIDTWFD SGSASYAAMH YPFSKDFTKS HFPVDFITEA
560 570 580 590 600
IDQTRGWFYT LHVVASLLFD ENAYKNVVSI SHILDENGQK MSKSKGNFIA
610 620 630 640 650
AIDFLNDYGA DAARMFFFTG APWNSKSVNK KLIGEITRKN LSTLLNVYSF
660 670 680 690 700
FASNANIDEY RFTEIKEPEN LLDRWMLSRL NTTIIKVREN MDNYNIHTAL
710 720 730 740 750
RYIEDLISEL SNVYLRLSRK RFWEGNLDDS KERAYSTLYY TLRETIKMMA
760 770 780 790 800
PITPFFSEYL YQKLSPGMPS VHMESYPEAI ERFIDNDLEN EMEHAIEIME
810 820 830 840 850
LSRRTRQELN IKGRQPVKEI LIYSDIKLRD DIIDIISPEL NAESIRFIKS
860 870 880 890 900
DEMPLKITVR ADISKVAKLL KSRINDFNLY LERNNDLVYR ELKSKGKINF
910 920 930 940 950
DGIYLTDDMF IMNEEVNGNY GFNKDERSGI YLFINREIDN DLLLEGYARE
960 970 980 990 1000
IIRRIQVMRK DLNLEYSEKI KTYIDADEDI RSAVERYMEK IKNETLSSEI
1010 1020
LFKNDPEARA WDIDDKTVYI KIVK
Length:1,024
Mass (Da):120,191
Last modified:July 5, 2004 - v1
Checksum:i6B23F804B7AAFF9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA. Translation: AAT42996.1.
RefSeqiWP_011177212.1. NC_005877.1.
YP_023189.1. NC_005877.1.

Genome annotation databases

EnsemblBacteriaiAAT42996; AAT42996; PTO0411.
GeneIDi2844251.
KEGGipto:PTO0411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA. Translation: AAT42996.1.
RefSeqiWP_011177212.1. NC_005877.1.
YP_023189.1. NC_005877.1.

3D structure databases

ProteinModelPortaliQ6L206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi263820.PTO0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT42996; AAT42996; PTO0411.
GeneIDi2844251.
KEGGipto:PTO0411.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-426-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Entry informationi

Entry nameiSYI_PICTO
AccessioniPrimary (citable) accession number: Q6L206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.