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Q6L206

- SYI_PICTO

UniProt

Q6L206 - SYI_PICTO

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PTO0411
Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPTOR263820:GHA3-426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PTO0411
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
ProteomesiUP000000438: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098586Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi263820.PTO0411.

Structurei

3D structure databases

ProteinModelPortaliQ6L206.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiNANIDEY.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6L206-1 [UniParc]FASTAAdd to Basket

« Hide

MKATLYENIN VNKSMIDINN EILNYWKENH IDENIIKSNR NLKPFAFLEG     50
PPTANGRPHV GHLMTRAVKD TVMRYKYMTG HDILRRTGGW DCHGLPVELE 100
AEKHFGFKNK KDIENFGIEK FNQYCRDSVF RYIDEWNIVD DLVGFWVDKE 150
NSYITLKNDY MESEWWALKT LYENNLLVKD YKIVPYCPRC GTSLSSHEVA 200
QGYKNVDDPS VFVKFAEKGR KNRYFIAWTT TPWTLPSNEF LVVNPDMDYS 250
LIESDGFEYY LLSSKVESLF NDYKLIKTFK GRDLEGIEYE QLMPFLEKPE 300
NAFRVVAASF VTAEDGTGIV HAAPAFGADD FEIGKRFSVE ILNPVDQDGR 350
FNEKLPWSGL FVTDANKSII NYLKENNLLF KAETMKHDYP FCYRCGTRLL 400
YYPLDTWFIK VSLIRKKLLE NNEKINWVPD YLKNGRFGNF LEEAKDWALS 450
RDRYWGTPLP IWRCNKNHYL AIGSRDDLLK YGGYIPEDLH RPYIDDVVLK 500
CPECGSEMHR ESYVIDTWFD SGSASYAAMH YPFSKDFTKS HFPVDFITEA 550
IDQTRGWFYT LHVVASLLFD ENAYKNVVSI SHILDENGQK MSKSKGNFIA 600
AIDFLNDYGA DAARMFFFTG APWNSKSVNK KLIGEITRKN LSTLLNVYSF 650
FASNANIDEY RFTEIKEPEN LLDRWMLSRL NTTIIKVREN MDNYNIHTAL 700
RYIEDLISEL SNVYLRLSRK RFWEGNLDDS KERAYSTLYY TLRETIKMMA 750
PITPFFSEYL YQKLSPGMPS VHMESYPEAI ERFIDNDLEN EMEHAIEIME 800
LSRRTRQELN IKGRQPVKEI LIYSDIKLRD DIIDIISPEL NAESIRFIKS 850
DEMPLKITVR ADISKVAKLL KSRINDFNLY LERNNDLVYR ELKSKGKINF 900
DGIYLTDDMF IMNEEVNGNY GFNKDERSGI YLFINREIDN DLLLEGYARE 950
IIRRIQVMRK DLNLEYSEKI KTYIDADEDI RSAVERYMEK IKNETLSSEI 1000
LFKNDPEARA WDIDDKTVYI KIVK 1024
Length:1,024
Mass (Da):120,191
Last modified:July 5, 2004 - v1
Checksum:i6B23F804B7AAFF9F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42996.1.
RefSeqiWP_011177212.1. NC_005877.1.
YP_023189.1. NC_005877.1.

Genome annotation databases

EnsemblBacteriaiAAT42996; AAT42996; PTO0411.
GeneIDi2844251.
KEGGipto:PTO0411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017261 Genomic DNA. Translation: AAT42996.1 .
RefSeqi WP_011177212.1. NC_005877.1.
YP_023189.1. NC_005877.1.

3D structure databases

ProteinModelPortali Q6L206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 263820.PTO0411.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT42996 ; AAT42996 ; PTO0411 .
GeneIDi 2844251.
KEGGi pto:PTO0411.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi NANIDEY.

Enzyme and pathway databases

BioCyci PTOR263820:GHA3-426-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Entry informationi

Entry nameiSYI_PICTO
AccessioniPrimary (citable) accession number: Q6L206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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