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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei593ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPTOR263820:G1G0U-449-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PTO0411
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
Proteomesi
  • UP000000438 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985861 – 1024Isoleucine--tRNA ligaseAdd BLAST1024

Proteomic databases

PRIDEiQ6L206

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi263820.PTO0411

Structurei

3D structure databases

ProteinModelPortaliQ6L206
SMRiQ6L206
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi52 – 62"HIGH" regionAdd BLAST11
Motifi590 – 594"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q6L206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKATLYENIN VNKSMIDINN EILNYWKENH IDENIIKSNR NLKPFAFLEG
60 70 80 90 100
PPTANGRPHV GHLMTRAVKD TVMRYKYMTG HDILRRTGGW DCHGLPVELE
110 120 130 140 150
AEKHFGFKNK KDIENFGIEK FNQYCRDSVF RYIDEWNIVD DLVGFWVDKE
160 170 180 190 200
NSYITLKNDY MESEWWALKT LYENNLLVKD YKIVPYCPRC GTSLSSHEVA
210 220 230 240 250
QGYKNVDDPS VFVKFAEKGR KNRYFIAWTT TPWTLPSNEF LVVNPDMDYS
260 270 280 290 300
LIESDGFEYY LLSSKVESLF NDYKLIKTFK GRDLEGIEYE QLMPFLEKPE
310 320 330 340 350
NAFRVVAASF VTAEDGTGIV HAAPAFGADD FEIGKRFSVE ILNPVDQDGR
360 370 380 390 400
FNEKLPWSGL FVTDANKSII NYLKENNLLF KAETMKHDYP FCYRCGTRLL
410 420 430 440 450
YYPLDTWFIK VSLIRKKLLE NNEKINWVPD YLKNGRFGNF LEEAKDWALS
460 470 480 490 500
RDRYWGTPLP IWRCNKNHYL AIGSRDDLLK YGGYIPEDLH RPYIDDVVLK
510 520 530 540 550
CPECGSEMHR ESYVIDTWFD SGSASYAAMH YPFSKDFTKS HFPVDFITEA
560 570 580 590 600
IDQTRGWFYT LHVVASLLFD ENAYKNVVSI SHILDENGQK MSKSKGNFIA
610 620 630 640 650
AIDFLNDYGA DAARMFFFTG APWNSKSVNK KLIGEITRKN LSTLLNVYSF
660 670 680 690 700
FASNANIDEY RFTEIKEPEN LLDRWMLSRL NTTIIKVREN MDNYNIHTAL
710 720 730 740 750
RYIEDLISEL SNVYLRLSRK RFWEGNLDDS KERAYSTLYY TLRETIKMMA
760 770 780 790 800
PITPFFSEYL YQKLSPGMPS VHMESYPEAI ERFIDNDLEN EMEHAIEIME
810 820 830 840 850
LSRRTRQELN IKGRQPVKEI LIYSDIKLRD DIIDIISPEL NAESIRFIKS
860 870 880 890 900
DEMPLKITVR ADISKVAKLL KSRINDFNLY LERNNDLVYR ELKSKGKINF
910 920 930 940 950
DGIYLTDDMF IMNEEVNGNY GFNKDERSGI YLFINREIDN DLLLEGYARE
960 970 980 990 1000
IIRRIQVMRK DLNLEYSEKI KTYIDADEDI RSAVERYMEK IKNETLSSEI
1010 1020
LFKNDPEARA WDIDDKTVYI KIVK
Length:1,024
Mass (Da):120,191
Last modified:July 5, 2004 - v1
Checksum:i6B23F804B7AAFF9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA Translation: AAT42996.1
RefSeqiWP_011177212.1, NC_005877.1

Genome annotation databases

EnsemblBacteriaiAAT42996; AAT42996; PTO0411
GeneIDi2844251
KEGGipto:PTO0411
PATRICifig|263820.9.peg.436

Similar proteinsi

Entry informationi

Entry nameiSYI_PICTO
AccessioniPrimary (citable) accession number: Q6L206
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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