Q6L1K9 (DP2L_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	PTO0558

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	1080 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1080	1080	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294699

Sequences

	Sequence		Length	Mass (Da)
	Q6L1K9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 02273EA4918062BC	FASTA	1,080	121,576
	10 20 30 40 50 60 MIDHSLFDYN ERLRKETYEC YEIAKKARAM GLDVSDEVEI PIANDMAERV EELIHISGIA 70 80 90 100 110 120 GEIRELSKSM SREELSLHIS KKVASLLKDN RVEALDKAVR VGLAILTEGI LVAPLEGIKD 130 140 150 160 170 180 VKINKNDDGT EYVSIVYSGP IRSAGGTAQA LSVLIADIVR RELNIGSFSP TDDEIERYIE 190 200 210 220 230 240 EVEAYNRLKH LQYMPTPDEI RLVLKNSPVM IDGEGSEEEE VSGHRDMKRI TTNRIRGGMC 250 260 270 280 290 300 LVLCEGLIQK AKKVLKYTNV MHLNEWNILE NIGKNKSDEK TENKSEKYLK DIIAGRPVFG 310 320 330 340 350 360 YPNRPGGFRL RYGRSRVSGL AAASINPVTM KILNDFIAIG SQIKVELPGK AAAITPCDSI 370 380 390 400 410 420 DGPMVLTRSG DHIKVKSIEQ AEKIKNDIER ITDLGEILIA YGDFLENNRN LDLSPFTREW 430 440 450 460 470 480 WEYYLNDELS IYKSRDPDQF DAVNISRKYK MPMFPGYDYF WHDITMEELN LLINAIANGI 490 500 510 520 530 540 INDDSMMLDA SVSEILIKLG IEFKKHNNKL ILHEYYPLIV SCGFDLINEK IVKVSDERKN 550 560 570 580 590 600 DVLGTVNALS GIEIKPRAPV RVGARLGRPE KAGDRKMKPK VHGLFPLMNY GGSTRSIINA 610 620 630 640 650 660 ARSGSIMDIE LGARICRACG TETPFVRCPK CGMPTEDSDS EKKFKIDISK ALDDAALRVS 670 680 690 700 710 720 TDIGTIKELK GVKKLMSRRS VIEPLEKAIL RAKHGISINK DGTCRYDMSD IPITHFKYNE 730 740 750 760 770 780 ISLTRQRLME LGYSDSDINE IYPQDIIIPR DAASYLLNVS KFIDDLLVNY YGLGPYYMCN 790 800 810 820 830 840 SEDDLIGHLV IGLAPHTSGG IVGRIIGFSD VNGCYAHPFF HAAKRRNCDG DEDSIMLLLD 850 860 870 880 890 900 GLLNFSKKYL PSTTGGLMDA PLVLTLILDP EEIDKEALNV DTLQRYPLDF YIATEKNAPP 910 920 930 940 950 960 ASIENMMKTM KVLIKDGRYT GISYSFDTGD ISYGTRLSAY KTIGSMEEKI EKQLGLARIL 970 980 990 1000 1010 1020 RSVDENDVAA RVLSSHFLPD IYGNFRSFFT QEFRCTKCNA KYRRVPLSGR CLRCGSSNII 1030 1040 1050 1060 1070 1080 LTIHHGSIVK YLNETKKVMN EYKLPDYLVF RINRLLEQIE STFDIENGND TTLDALIENE

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43143.1.
RefSeq	YP_023336.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2844296.
GenomeReviews	Gene locus PTO0558 in contig AE017261_GR.
KEGG	pto:PTO0558.
NMPDR	fig|263820.1.peg.558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0558-MONOMER.
BRENDA	2.7.7.7. 276518.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_PICTO

Accession

Primary (citable) accession number: Q6L1K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 5, 2004
Last modified:	August 10, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)