Q6L195 (DNLI_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	PTO0672

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.
Sequence caution	The sequence AAT43257.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	DNA ligase HAMAP MF_00407		PRO_0000059610
Sites
	Active site	250	1	N6-AMP-lysine intermediate By similarity
	Binding site	248	1	ATP By similarity
	Binding site	255	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	300	1	ATP By similarity
	Binding site	341	1	ATP By similarity
	Binding site	418	1	ATP By similarity
	Binding site	424	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6L195 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: 9AED5BCA48C11F94	FASTA	585	66,334
	10 20 30 40 50 60 MDFSDVASYF SRMEETTKRL ELTSILAELF KRSGDDLKKL VYLVQGKLMP DYLGIELGLS 70 80 90 100 110 120 DKLIIKSLSK ASGRSEEDIN RIFSRLGDLG STAEEISSSG IQRPLLKESL TVDYVYNQLI 130 140 150 160 170 180 KISGYTGHGS IKTKMDAYID LLINSGPMEI KYITRIITGK LRLGVADSTI LDGLIEAFSE 190 200 210 220 230 240 KKYADDIETA YNFHPDLGYI AENLMMGNIN ELLNAGPVPL IPFKVMLAER LQSISDIRNK 250 260 270 280 290 300 MGHNASYEYK YDGLRTQLHF LKGGIKIFSR GLEETTSSFP DIVQNFKSYY SFDSCIIDGE 310 320 330 340 350 360 SVPYNPETGE LYPFQMVSKR RGRKYELTEK TREVPIVMFI FDILYLNGKS LVNLPYPERR 370 380 390 400 410 420 SILEKNFKEN EYFKLAKRIV SDDEHDIMKF FERSIEEGCE GIVAKSNGID SIYRAGARGW 430 440 450 460 470 480 LWIKFKRDYQ SELSDSLDLV VVGAFDGHGR RKGTFGALLL ACYNSKDDTF ETVCKLGSGF 490 500 510 520 530 540 TDEMLSEMPR LLGDKIVEKK PARVNSSMEP DHWIYPSLVL EIRGAEITVS PVHTCAMNII 550 560 570 580 EKGSGLALRF PRLIKPRDDK KPEDATTTNE IIEMYKAQKK VIEKS

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43257.1. Different initiation.
RefSeq	YP_023450.1. NC_005877.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2845337.
GenomeReviews	Gene locus PTO0672 in contig AE017261_GR.
KEGG	pto:PTO0672.
NMPDR	fig|263820.1.peg.672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG570821.
OMA	LAPAYEG.
ProtClustDB	PRK01109.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0672-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. False negative. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_PICTO

Accession

Primary (citable) accession number: Q6L195

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	March 3, 2009
Last modified:	November 16, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program