Reviewed, UniProtKB/Swiss-Prot Q6L195 (DNLI_PICTO)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	PTO0672

Organism

Picrophilus torridus [Complete proteome] [HAMAP]

Taxonomic identifier

82076 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	DNA ligase HAMAP MF_00407		PRO_0000059610
Sites
	Active site	255	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6L195-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5DF71B6B15B51439	FASTA	590	66,969
	10 20 30 40 50 60 MLYINMDFSD VASYFSRMEE TTKRLELTSI LAELFKRSGD DLKKLVYLVQ GKLMPDYLGI 70 80 90 100 110 120 ELGLSDKLII KSLSKASGRS EEDINRIFSR LGDLGSTAEE ISSSGIQRPL LKESLTVDYV 130 140 150 160 170 180 YNQLIKISGY TGHGSIKTKM DAYIDLLINS GPMEIKYITR IITGKLRLGV ADSTILDGLI 190 200 210 220 230 240 EAFSEKKYAD DIETAYNFHP DLGYIAENLM MGNINELLNA GPVPLIPFKV MLAERLQSIS 250 260 270 280 290 300 DIRNKMGHNA SYEYKYDGLR TQLHFLKGGI KIFSRGLEET TSSFPDIVQN FKSYYSFDSC 310 320 330 340 350 360 IIDGESVPYN PETGELYPFQ MVSKRRGRKY ELTEKTREVP IVMFIFDILY LNGKSLVNLP 370 380 390 400 410 420 YPERRSILEK NFKENEYFKL AKRIVSDDEH DIMKFFERSI EEGCEGIVAK SNGIDSIYRA 430 440 450 460 470 480 GARGWLWIKF KRDYQSELSD SLDLVVVGAF DGHGRRKGTF GALLLACYNS KDDTFETVCK 490 500 510 520 530 540 LGSGFTDEML SEMPRLLGDK IVEKKPARVN SSMEPDHWIY PSLVLEIRGA EITVSPVHTC 550 560 570 580 590 AMNIIEKGSG LALRFPRLIK PRDDKKPEDA TTTNEIIEMY KAQKKVIEKS

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017261 Genomic DNA. Translation: AAT43257.1.
RefSeq	YP_023450.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2845337.
GenomeReviews	Gene locus PTO0672 in contig AE017261_GR.
KEGG	pto:PTO0672.
NMPDR	fig|263820.1.peg.672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6L195.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0672-MON.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. False negative. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_PICTO

Accession

Primary (citable) accession number: Q6L195

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)