Reviewed, UniProtKB/Swiss-Prot Q6L125 (G3P_PICTO)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	PTO0742

Organism

Picrophilus torridus [Complete proteome] [HAMAP]

Taxonomic identifier

82076 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145727
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	138 – 140	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	192 – 193	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	139	1	Nucleophile By similarity
	Binding site	109	1	NAD; via amide nitrogen By similarity
	Binding site	167	1	NAD By similarity
	Binding site	169	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	299	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6L125-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 388F45B97ECFDA93	FASTA	341	38,559
	10 20 30 40 50 60 MIKVGINGYG TIGKRVAYAA SIQDDIHVSG IVKNTPDYMA YLASRSFNIY VPDDDKIKEF 70 80 90 100 110 120 EDHGIKVKGT LNDLMESSDI IVDATPEGMG MENIKIYKKK RVKAIFQGGE KSNIGDASFN 130 140 150 160 170 180 AYSNYNESFK REYTRVVSCN TTALARTLYP ILNDYGIENL NVTLIRRATD PNDNRKGPIN 190 200 210 220 230 240 AIEPSMSFPS HHADDLKTVL NLNNVYTVAL KAPTTLMHVH SIEVLLKDDA KIDDIMESWL 250 260 270 280 290 300 KYNRILLIEG KDNFKSTAQI MDMARELRRD RGDLYEIAIW RDSVKVIDKR LYYVQAVHQE 310 320 330 340 SDVIPENIDA IRSLSGIDKN ESIEKTDKSL RISGGIFYGK E

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017261 Genomic DNA. Translation: AAT43327.1. Different initiation.
RefSeq	YP_023520.1.
3D structure databases
HSSP	HSSP built from PDB template 1B7G based on UniProtKB P39460.
ModBase	Search...
Genome annotation databases
GeneID	2845092.
GenomeReviews	Gene locus PTO0742 in contig AE017261_GR.
KEGG	pto:PTO0742.
NMPDR	fig|263820.1.peg.742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6L125.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0742-MON.
BRENDA	1.2.1.59. 276518.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_PICTO

Accession

Primary (citable) accession number: Q6L125

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)