Q6L0E8 (RIFK_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	PTO0969

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Riboflavin kinase HAMAP MF_01285		PRO_0000322097
Regions
	Nucleotide binding	99 – 104	6	CDP By similarity
	Nucleotide binding	197 – 200	4	CDP By similarity
	Region	1 – 89	89	H-T-H motif-like HAMAP MF_01285
	Region	90 – 221	132	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	128	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity
	Binding site	185	1	FMN By similarity
	Binding site	192	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6L0E8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B5C82FDEC8A59521	FASTA	221	25,269
	10 20 30 40 50 60 MENIYIALKT IKKMAGEKNT VFISSKELAN EMNVSQQTAS RIIISLDRLG YITRTLENRK 70 80 90 100 110 120 QKITINDSGL DVLYREYNEI SSILRFSQEL KLVGAVQDGL GEGKYYISKK GYKDQFIAKL 130 140 150 160 170 180 GIDPYPGTLN IKILNEYENN LRRIKNSDGI YITGFKDQQR TFGGVFCHAA RIDGIRCFII 190 200 210 220 FPERSVYRDT FEVISEKYLR KELNLENGAI IEVLASIDGK L

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43554.1.
RefSeq	YP_023747.1. NC_005877.1.
3D structure databases
ProteinModelPortal	Q6L0E8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2844213.
GenomeReviews	Gene locus PTO0969 in contig AE017261_GR.
KEGG	pto:PTO0969.
NMPDR	fig|263820.1.peg.969.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	PRK14165.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0969-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PICTO

Accession

Primary (citable) accession number: Q6L0E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program