Q6L0D1 (NADE_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	PTO0986

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152228
Regions
	Nucleotide binding	28 – 35	8	ATP By similarity
Sites
	Active site	30	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6L0D1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0D4E6AC8AA1C27DE	FASTA	249	28,519
	10 20 30 40 50 60 MVERLDGVFK SISDFLRQEL NGKNAVIGVS SGIDSALVLT ILSKAIDKDR IHAFFMPDRF 70 80 90 100 110 120 TRSADFDDIR SLEGSTGVKI NEINIENIVN GYKSTLGIND KKYEGNIRSR VRSVILYYNA 130 140 150 160 170 180 NLLNGLVVGT TNRTEYLIGY FTKYGDGACD LEPIEHLYKS DVRELASYLK VPESIIRKKP 190 200 210 220 230 240 SAGLWGDQYD EDELGMGYEE LDSILKDLFE KKTGILDDRY KMVYDMYIRS QHKRKLPKSM MNDDFRYNV

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43571.1.
RefSeq	YP_023764.1. NC_005877.1.
3D structure databases
ProteinModelPortal	Q6L0D1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2844813.
GenomeReviews	Gene locus PTO0986 in contig AE017261_GR.
KEGG	pto:PTO0986.
NMPDR	fig|263820.1.peg.986.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	Q6L0D1.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO0986-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_PICTO

Accession

Primary (citable) accession number: Q6L0D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program